EMAGE mouse gene expression spatial database

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EMAGE:19559

Mthfd1l methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like ( MGI:1924836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002080. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002080_01	euxassay_002080_02	euxassay_002080_03	euxassay_002080_04

euxassay_002080_05	euxassay_002080_06	euxassay_002080_07	euxassay_002080_08	euxassay_002080_09

euxassay_002080_10	euxassay_002080_11	euxassay_002080_12	euxassay_002080_13	euxassay_002080_14

euxassay_002080_15	euxassay_002080_16	euxassay_002080_17	euxassay_002080_18	euxassay_002080_19

euxassay_002080_20	euxassay_002080_21	euxassay_002080_22	euxassay_002080_23	euxassay_002080_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19559_wholemount_strong.wlz

19559_wholemount_moderate.wlz

19559_wholemount_weak.wlz

19559_wholemount_possible.wlz

19559_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19559_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19

foregut-midgut junction

moderate

regional

moderate expression: see section 11 12 13

vibrissa

moderate

regional

moderate expression: see section 05 06 07 20 21 22

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 16 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24

hindgut

moderate

regional

moderate expression: see section 11 12 13

rectum

moderate

regional

moderate expression: see section 15

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 21 22 weak expression: see section 05

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 21 22 weak expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5474
Entity Detected:	Mthfd1l, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like ( MGI:1924836)
Sequence:	sense strand is shown >T5474 CCACGCGTCCGCAAAGTTCTCAATGCTTTGAAACCAGAAAAAGATGTGGACGGGGTGACCGACATCAATC TGGGGAAGCTCGTACGAGGTGACGCTCCGGAGTGTTTTGTTTCACCCTTGGCCAAAGCTGCTGTAGAACT TGTTGAAAAGTCAGGGATCACTTTGGATGGAAAGAAGGTCTTGGTCGTAGGGGCGCACGGGCCCCTGGAA GCTGCCCTGCAGTGGCTTTTCCAGAGAAAAGGATCTATGACAATGAGCTGCCCCTGGGCAACACCTCAGC TTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCTTAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGC GTGGATTCCATCAGGAACCACCATTCTCAATTGCTTCCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGT TCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAAGAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCA TTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCAGAGAGCAGCAACATCGAAGATGGCGACTGCACTG CTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGATATTGAGATTTCTCGAGGACAGACTCCAAAAGCT GTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAGAGCAAAGCCA AAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATCAAACAGATGGAAAATACGTCTTGGTTGCTGGGAT CACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGTCACCATCGGACTAGTGCAGGCGCTGACCGCTCAC CTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCTTCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAG CTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGGAGGAGTTCAACCTTCACCTGACTGGGGACATCCA TGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGCCATCGACACGAGAATTTTACATGAGAGCACTCAA ACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAAC TTTCTCGGCTGAAGAAACTGGGGATACATAAGACTGATCCAAGCACACTGACCGAGGAGGAAGTGAGGAA GTTCGCCCGCCTCAACATTGACCCCGCAACCATCACGTGGCAGAGAGTATTGGACACGAACGACCGATTT CTGAGAAAAATAACCATTGGACAGGGAAGTACAGAGAAAGGATATTCCCGGCAGGCACAGTTTGACATTG CAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGACTGATAGCCTCACAGACATGAAAGAGCGGCTAGG AAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCCTGTGACAGCTGAGGACTTGGGGGTGACCGGCGCT CTGACAGTTTTGATGAAAGACGCCATCAAGCCAAATCTGATGCAGACCCTTGAAGGAACACCTGTGTTTG TGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCAACTCATCAGTATTAGCTGATAAAATCGCGCTGAA ATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGCTGGCTTTGGGGCTGATATTGGGATGGAGAAATTC TTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCCAACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTC TGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTGGGGTTCCTCTCAAGAAAGAATATACAGAGGAGAA CATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACAGAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTG CCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGACACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCG CTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCTATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGA CTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAGAAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTT CCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACTGTCTATGGAGCCAAGGACATTGAGCTGTCTCCTG AGGCGCAGTCCAAAATCGATCGCTATACGCAACAGGGTTTTGGAAATTTGCCCATTTGCATGGCGAAGAC CCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGGAGTGCCGAGGGATTTCACTCTGCCCATCAGTGAT GTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCTTTGGTGGGAACGATGAGCACAATGCCAGGACTGC CCACCCGACCCTGCTTTTATGATATAGACCTTGACACAGAAACGGAGCAAGTTAAAGGCTTGTTCTAATG GAATGAAACCCACAGGACTGGGCAAGAAAACAGCTCACACAAGAATCCCAGCCTTTAAAACCACAACACA AGTAAGAGTTTAACACCAGAGGAAAAGACGACTTGGCTCTGAGACCCCACCCCCTCTTTTTTCAGAGTCT TCAAACAAAAAACGTCTCCTTGCCTTTTTGCTGCAGTTGGAGAAGAAAATGAATTTTGAAATCTTGTGAT GCAATGCTGGAGCAATCCTGAGACAGGCCAAAGACCGCTTCCTCATACAAGATTAACTCTGTTTCCAGCC AAGTGGTGTTCAACAGAAGGACCTGCGCCACATCTGGAAGGAGTGAAGTTACTCTTGGTTCCGATAGTGT TTACACCATTTCTGCTGCTTATACTTTAGAAAGTTTATTTTATGGGGACTAGGGCTTTGCAAGAGTGTGA ACTAAAAGCTAATGCTTCCGTCTTTGGAGTTTTCTCTTTCATCTTTTTTATTCTTCCTTTCCTCACCTGT GTTGAGGATGGGATGTCAGGCCTCTGCTTGCACCGTCCAGCGCTGTGCTGCCAAACTGTATTTGTTTTTG TCTTTGAACTGAAAATAAACATGTGTTCTAGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374413 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374413 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19557 same embryo
	EMAGE:19556 same embryo
	EMAGE:19560 same embryo
	EMAGE:19558 same embryo
	EMAGE:19555 same embryo
	EurExpress:euxassay_002080 same experiment
	MGI:4826491 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source