EMAGE mouse gene expression spatial database

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EMAGE:19590

AU040320 expressed sequence AU040320 ( MGI:2140475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005035. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005035_01	euxassay_005035_02	euxassay_005035_03	euxassay_005035_04

euxassay_005035_05	euxassay_005035_06	euxassay_005035_07	euxassay_005035_08	euxassay_005035_09

euxassay_005035_10	euxassay_005035_11	euxassay_005035_12	euxassay_005035_13	euxassay_005035_14

euxassay_005035_15	euxassay_005035_16	euxassay_005035_17	euxassay_005035_18	euxassay_005035_19

euxassay_005035_20	euxassay_005035_21	euxassay_005035_22	euxassay_005035_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19590_wholemount_strong.wlz

19590_wholemount_moderate.wlz

19590_wholemount_weak.wlz

19590_wholemount_possible.wlz

19590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 09 18 19

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 13 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 11 12 13 15

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 weak expression: see section 08 not examined expression: see section 13

hindgut

moderate

regional

moderate expression: see section 13

rectum

moderate

regional

moderate expression: see section 13

midgut

weak

regional

weak expression: see section 14 15 16 17 18

midgut loop

moderate

regional

moderate expression: see section 11 12 13 weak expression: see section 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7465
Entity Detected:	AU040320, expressed sequence AU040320 ( MGI:2140475)
Sequence:	sense strand is shown >T7465 CGGACGCGTGGGTTTCCGGCCGCGGTCGCTGTCCAGGGAGGCTGAGGCAAGAGGGAGCTGTCCGGGTGGG GAGCCAGCACTTCCTTCTTCCTCCTCTGCGTGAGGGGAGAGAAGGTTGGGGGTCCCCGAGCCCATGGATC GGGAGGAGGCGGAGGCCGCCGAGAGCCGGCACCCCTCTATGTGGCCCTGAGCCCCGTGTACTGGTTCCGC CTCTCTGGAAGGCCATGGAGAAGAGACTGGGAGTCAAGCCAAGTCCCGCTTCCTGGGTTTTGCCAGGATA TTGTTGGCAGACATCAGTGAAGCTGCCGAGAAGCCTGTACCTGCTTTACAGTTTCTTCTGCTTCAGCGTT CTGTGGTTGTCAACAGATGCTGATGAGAGCAGATGCCAACAGGGGAAGACACTTTATGGAGCTGGCTTGA GAACTGAGGGAGAAAATCACCTCCGGCTTCTTGCAGGAAGCCTGCCTTTCCACGCCTGTCGGGCTGCCTG CTGCCGGGACTCTGCCTGCCACGCTCTATGGTGGCTGGAAGGGATGTGCTTTCAGGCTGACTGCAGCAAG CCCCAGAGCTGCCAGCCTTTTAGGACAGACTCTTCCAATTCCATGCTGATCATTTTTCAAAAATCCCAAA CTACAGATGATTTGGGCCTTCTGCCTGAAGATGATGAACCACATCTTCTGAGGCTAGGCTGGGGCAGGAC ATCGTGGAGGAGGCAGAGCCTTCTTGGGGCTCCCCTCACCCTTTCTGTACCCTCTAGTCACCACCAGAGC TTACTCAGGGATCGGCAGAAGAGAGATCTCAGTGTGGTACCTACACATGGAGCGATGCAGCATTCTAAAG TGAATCACTCCGAGGAAGCAGGTGCTCTGAGTCCCACCTCTGCAGAGGTCCGCAAAACCATTACAGTTGC CGGTTCCTTCACCAGTAACCACACTACACAGACTCCTGAGTGGCCCAAGAATGTGTCCATCCATCCTGAA CCATCCGAGCACTCCAGTCCTGTATCTGGTACTCCGCAAGTAAAAAGCACTGAGCACAGTCCAACTGATG CCCCTCTGCCAGTGGCCCCCTCCTACAGCTATGCCACCCCCACGCCCCAGGCCTCTTCTCAGAGCACCTC AGCACCACACCCAGTTGTAAAGGAGCTGGTGGTGTCTGCTGGGAAGAGCGTCCAGATCACCCTGCCTAAG AATGAAGTTCAGTTAAATGCCTTCGTCCTTCCAGAAGCAGAGCCAGGAGAAACCTACACCTACGACTGGC AGCTGATCACTCATCCTACAGACTACAGTGGAGAGGTGGAGAGGAAACATTCCCAGAGCCTCCAACTGTC CAAGCTGACTCCAGGCCTGTACGAATTCAAGGTGACTGTGGATGGCCAGAATGCCCATGGGGAAGGCTAC GTGAATGTGACAGTGAAACCAGAGCCCCGTAAGAACCGGCCTCCCGTTGCTGTGGTGTCACCTCAGTTCC AGGAGATCTCGCTGCCAACCACTTCTACCATCATTGATGGCAGCCAGAGCACGGATGACGATAAAATTGT CCAGTACCACTGGGAAGAGCTTAAGGGGCCCCTGAGAGAAGAGAAGATCTCTGAAGACACAGCCATACTA AAACTTAGTAAGCTCGTCCCGGGGAACTACACCTTCAGCTTAACTGTTGTCGACTCTGACGGGGCTACCA ACTCCACCACTGCAAGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCCAA CCAAGTGATCACCCTGCCTCAGAACTCCATCACCCTCTTTGGAAACCAGAGCACGGATGACCACGGCATC ACCAGCTATGAGTGGTCGCTCAGCCCGAGCAGCAAAGGGAAGGTGGTGGAGATGCAGGGAGTTAGAACGC CAGCCCTGCAGCTGTCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGG ACAACAGGCCACCGCCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCA GGCCCAGACAAAGAGCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACC AGAGAGTCGTCTCTTACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAG CAGTGTCGCCACTGTGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGG AACCTACAGAGCCAGAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGA TCGCTGGGAACGTGGTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGA CAAGGGGATAGTCAGCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCAC TCTGACCACCACCCTGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGA CAGATGCAAAGGGCGAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAA CCTAGTGGAGATCATCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGC CAGATTGGGGTCCTCCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGC AGAGCACCAAGATGTTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGC AGCCATGCTCAAGAGCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGC ACAGTCACATGTCAGCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTG ACCCGTTTTGGATGGAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGT GCTCTACGTCATCATTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGC TGCTGCAAGAGGCAAAAAGGAAAACCCAAGAGGAAAAGCAGATACAAGATCCTGGATGCCACAGATCAGG AGAGCCTGGAGCTGAAACCAACCTCCCGAGCAGGCAGCAAACAGAAAGGCCCCACGCTGAGCAGCAGCCT GATGCATTCTGAATCGGAGCTGGACAGCGACGATGCCATCTTCACATGGCCAGACCGGGAGAAGGGCAAA CTACTGTATGGTCAGAATGGCTCTGTGCCAAACGGGCAAACACCTTTGAAGTCCAGGAGCGCACGGGAGG AGATCTTGTAGCCCCCTGATCTGTCTCAACAGTCTGGGCCTCAGTGCTAGGGGCCAGAGCACCTCTAGGC TGATTCCTCACCTTTTGGGTCTGAGGGCAGCTGCTCTCCCAGCATGTGCTGGCCACTCCATCCCAAAAGC CCCCATCAGAACTGCTGGTACTTGAATCCTCAGATACTTTATAGGAGCCCTTGAATGAAGCTGTGAATGG AGAGGTTTTCTCTATAAACCTATGTGGTAGCCCCCCCCCCGGCCCCCCAAATGTCCTCATCTCAGGGCCT CACTTTCTGCACACACCTCCCTGCCTGAGAGCAGACACAAACAGGCCCATAGCCTCTCCTCTGCTGTTAG AGCAATGCAGCTGGGCCCAGAACACCCATGATGAGGTTCACTGCGTCTACCCTGCACCTCGCACAGAACC AAGAGCCACCAGCCTCCTGATGGCAGGGGCCTAGCCCTGGGCATATTCATTTGTTTGCTTCTCTGGGACA GTATGCCCTGCCTTCCCTGGGCCAAAAGAAATCCGTGCTGTCACTGATGCTGGCGATGATGCAGAGCTGT CGCTGCCCTCAGCTCCTGCTGGCTTGTTTATCAGGGATATGGGAGCTGCGGGCATGACAGCCACATTCTC CCAGAAGAGACAGCCACAGATAGACTGGAACTCTCTGGCATGTGAGTGCATATGTGTGACTGCCCCACTG TGGGACCTGCCACATCCCTCCATCCATGTCTTTTCTGTGTCCTTTGCACGTGTGAGTGCTCGAGTGTATG TGCCCATTCCTCATAGGTCTCTTGGCTGCTCTCAAGGTTAATTTTAACTTGGAGACTTTCAGCTCCTTGA AAGACTATTAATCCCTCCAGCCTGATGACCAGTTCTCAGCTGTGGTGTGGCAGCTGAGGAATTGGGTTTC GGGGAGAGTGACTTTTTAAAAAGGACTTAATTTCTACTACTGCACTATCTGTTGTGAGATAATTAAACAT GCTATTTAATTGTGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235813 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235813 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19592 same embryo
	EMAGE:19589 same embryo
	EMAGE:19591 same embryo
	EurExpress:euxassay_005035 same experiment
	MGI:4823355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source