EMAGE mouse gene expression spatial database

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EMAGE:19629

Panx3 pannexin 3 ( MGI:1918881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005128. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005128_01	euxassay_005128_02	euxassay_005128_03	euxassay_005128_04

euxassay_005128_05	euxassay_005128_06	euxassay_005128_07	euxassay_005128_08	euxassay_005128_09

euxassay_005128_10	euxassay_005128_11	euxassay_005128_12	euxassay_005128_13	euxassay_005128_14

euxassay_005128_15	euxassay_005128_16	euxassay_005128_17	euxassay_005128_18	euxassay_005128_19

euxassay_005128_20	euxassay_005128_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19629_wholemount_strong.wlz

19629_wholemount_moderate.wlz

19629_wholemount_weak.wlz

19629_wholemount_possible.wlz

19629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 14 15 16 17 18 19 20 21

humerus

strong

regional

strong expression: see section 01 02 03

foot

strong

regional

strong expression: see section 08

tibia

strong

regional

strong expression: see section 01 02

femur

strong

regional

strong expression: see section 03 04 05 08 19 20 21

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 21 moderate expression: see section 02

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 17 18 19 21

palatal shelf

strong

regional

strong expression: see section 10 11 14 15 16 not examined expression: see section 12 13

cranium

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

not examined

regional

not examined expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

basisphenoid bone

strong

regional

strong expression: see section 11 12 13 weak expression: see section 14

frontal bone primordium

strong

regional

strong expression: see section 01

parietal bone

strong

regional

strong expression: see section 01 19 20 21

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 07 20 21 weak expression: see section 08 09

clavicle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 16 17 18 19 20

scapula

strong

regional

strong expression: see section 20 21

pelvic girdle skeleton

strong

regional

strong expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8069
Entity Detected:	Panx3, pannexin 3 ( MGI:1918881)
Sequence:	sense strand is shown >T8069 AAGAGGTAATCCGGCTGGTGCTGGGACACTCTCCGCTGAAAACTAGCTGCCTGAAGCTGTCACTCCACTG TACCTGGATCGCTTAGGGTAGCATTTTCCTCTGTATCCCCCCAAGTCCCCATTCTCAGCAGCATCATGTC GCTCGCACACACTGCTGCAGAGTACATGCTCTCTGATGCCCTGCTGCCTGACCGCAGGGGCTCTCGGCTC AAAGGACTGCGCCTGGAACTGCCCCTGGATAAGATGGTCAAGTTCATTACTGTGGGCTTTCCCTTGCTGC TGATGTCTCTAGCTTTCGCCCAGGAGTTCTCATCAGGGTCTCCGATCAGCTGTTTCTCTCCCAGCAACTT CAGTGTCAGGCAGGCTGCATACGTGGATAGCTCATGCTGGGACTCGCTGGCTCACCATACGCAGGACAAG GCTGGCCAGTACAAGGTGAAATCTCTCTGGCCTCACAAGGCTCTTCCCTACTCTCTACTGGCTCTGGCTG TGGCCATGTATCTGCCAGTGTTGCTGTGGCAATATGTAGCTGTGCCCTCCCTCAGCTCTGACCTGCTGTT CATCATCAGTGAGCTGGACAAGTCTTACAACCGTTCCATCCGCCTGGTGCAGCACATGTTGCAGATCCGG CAGAGCAGCTCTGACCCCCATGTCTTCTGGGATGAGCTAGAGAAGGCCCGGAAGGAACGGTACTTTGAAT TTCCCTTGCTAGAGCGGTACCTGGAATGCAAGCAGCGCTCCCATTGGCTAGTGGCCACCTACCTCCTGAG GAACGCCCTCTTACTCCTCTTTACCTCAGCCACTTACCTGTACCTTGGCCAATTCCATTTAGATGTCTTC TTTCAAGATGAATTCAACTGCTTCATCAAGACAGGGCTGCTACATGATGAGACCCATGTGCCAGAGCTGA TCACATGCAGGCTGACCTCCCTGTCTGTATTCCAGATTGTGAGTGTCTCCAGTGCAGCAATATACACCAT ATTGGTCCCAGTGATAATATACAACCTCACACGGCTATGTCGGTGGGACAAAGGACTCCTATCCATCTAT GAGATGCTCCCGGCTTTTGATCTCCTCAGTAGAAAGATGCTAGGATGCCCCATCAATGACCTCAATGTGA TTCTTCTTTTCCTCCGAGCTAATATCTCTGAGCTCATCTCTTTTAGCTGGCTGAGTGTCTTAAGTGTGTT GAAGGATACAACCACCCAGAAACACAACATCGACACAGTGGTTGACTTTATGACCTTTGTGGCTGGCTTA GAACCCTCAAAACCTAAACATCTCACCCAGCATACATATGATGAGCACGCATAGTTTAAAAAAAGAAACA CAGATAAGTTTTATAGAAAGTAACTCTCCTTCTTCACAAGCTATGCAGAGTAAAAGACAGAAAAATAATA AGGATGTCCACGTAAAATGGTTAAGATTGGATTCAGCCGAAATATCCTGTATTTTTAAGAAGATAAAAAT CAACACATGAAAATTCAGTGTGAAGTCTGGGAGTCAAGAGTCAAAGAACTGTAAAGTTCAGTTTAGAACA TTCAGAGGACAGTAGTGATAAAGGAACAAGCTGAAGCAAAGTGCAGTTATTGAACTCAGAAAAATTCTAA AGGCCATTTATGGAGGGAAATTCTCACAAGGCCCACCCGCTCTAGAAGTCATGCTTCCCTTCCCACAATG GGCAGGGTAGAACAATTTAATAAGAGATTAATAAGGATATGAACCCCTGAAACTCTTTGATTTGGCCTGC CCATCTTGCTTTGTGGACATTTTCAGCCTTGCCAAGCACTCTATGGCCTGCTAATAAGACAAGAAATAAT GATGGCTTTAACTTGTAATCTGCTTTTGGGACATAAGGACAGAGACAAGGCCATAAGTTGAATCTTTAGG AGTCCAATACGCTTTTTATGATATAAACTGAGGATTCTTTTCCACGAGGGCTCAGATTATGGACTATGAA CACCTTCTAAAATCTTAATGAAGCCTAGGTGTATTCCTAATAGTCCAAAGCATACTACAACCAACCAGTG AAGGTCAGTAGGTGAAAAGGCTGCTGCCAAGCCTGAATACCTGATTTCAATGCCTGGGACCCAAATGGTG AAAGGATAGAACCGATTCTCCAAGTTACCTTCTGATCTCCATACACAAGAAAAGTAAACAGAGGAGTATT AAGCAAGATGAACATGCAAGTCCCATCGATCCACTTCTATACGTTCTCCATTCACTGTTTCCTCCTTGCA TCTAATTGTCACAGCTTAGCTCTGATTCAAAAGACAGTGTTCTATCCCCAGTACCCTCGTGACTCTTAAT AAATATTTTTAAACTGTAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30058169 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30058169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19627 same embryo
	EMAGE:19631 same embryo
	EMAGE:19628 same embryo
	EMAGE:19630 same embryo
	EurExpress:euxassay_005128 same experiment
	MGI:4827039 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source