EMAGE mouse gene expression spatial database

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EMAGE:19686

Phf6 PHD finger protein 6 ( MGI:1918248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005159. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005159_01	euxassay_005159_02	euxassay_005159_03	euxassay_005159_04

euxassay_005159_05	euxassay_005159_06	euxassay_005159_07	euxassay_005159_08	euxassay_005159_09

euxassay_005159_10	euxassay_005159_11	euxassay_005159_12	euxassay_005159_13	euxassay_005159_14

euxassay_005159_15	euxassay_005159_16	euxassay_005159_17	euxassay_005159_18	euxassay_005159_19

euxassay_005159_20	euxassay_005159_21	euxassay_005159_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19686_wholemount_strong.wlz

19686_wholemount_moderate.wlz

19686_wholemount_weak.wlz

19686_wholemount_possible.wlz

19686_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19686_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

moderate

regional

moderate expression: see section 01 02 05 06 07 08 09 10 11 12 13 21 22 weak expression: see section 03 04

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

thymus primordium

strong

regional

strong expression: see section 09 10 11 12 13

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 15 16 17

brain

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

adenohypophysis

strong

regional

strong expression: see section 13 moderate expression: see section 12 weak expression: see section 14

vibrissa

strong

regional

strong expression: see section 05 21 moderate expression: see section 04 06 07 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 16 17 18 19 20 21 moderate expression: see section 04

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 16 17 18 19 21 moderate expression: see section 20

spinal cord

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 13

cervical ganglion

strong

regional

strong expression: see section 07 15

thoracic ganglion

strong

regional

strong expression: see section 07 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 05 06 11 12 13

eyelid

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 05

retina

strong

regional

strong expression: see section 01 02 03 22 moderate expression: see section 04 05

naris

strong

regional

strong expression: see section 11 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 12 13 14 15 16

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metanephros

strong

regional

strong expression: see section 04 05 06 07 08 13 14 15 16

genital tubercle of male

moderate

regional

moderate expression: see section 12 13 14 15 16 17

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

trachea

strong

regional

strong expression: see section 10 11

tail

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8826
Entity Detected:	Phf6, PHD finger protein 6 ( MGI:1918248)
Sequence:	sense strand is shown >T8826 CGGAGGGAGGCAACGTAAGGGCGCTCGGCGAGCCCGTCTCTCCTCGAATGAAAGGAAACTACCCCTGGCG ACAGAGCCCCGCTCTTCGGCAGTGCCGGAGAACCGACTCCCGCCTTCCCCGTCTTCCCTCGTGGGCGCTG TCCTCCCTTCGCCCAGCCGTGAGCCCCACTGCATTTCTTGAGGCTTAAAGTGGCAGTCTCTAAACACTTT AAAAATCATGTCAAGCTCAATTGAACAGAAAAAAGGGTCTACGAGACAGCGTAAATGTGGCTTTTGTAAG TCAAATAGAGACAAGGAATGTGGACAGTTACTGATATCTGAAAACCAGAAGGTGGCAGCACACCACAAGT GCATGCTGTTTTCGTCTGCTTTGGTGTCCTCACATTCTGATAATGAGAGTCTTGGTGGATTTTCTATTGA AGATGTTCAAAAGGAAATTAAAAGAGGCACAAAGCTGATGTGTTCTCTTTGTCATTGTCCTGGAGCAACC ATTGGCTGTGATGTGAAAACCTGCCACAGGACATACCACTACCACTGTGCATTGCATGATAAAGCTCAGA TCCGAGAGAAACCTTCGCAAGGGATTTACATGGTTTATTGTCGAAAACACAAGAAAACTGCACATAACTC GGAAGCTGATTTAGAAGAAAGTTTTAATGAGCATGAACTGGAGCCCTCATCTCCAAAGACTAAGAAGAAA AGTCGCAAAGGAAGACCGAGAAAAACTAACCTTAAAGGCTTGCCAGAAGACAGCAGGTCCACATCCTCCC ATGGAACAGATGAAATGGAAAGTAGCTCCTACCGAGATAGGTCTCCACACAGGAGCAGTCCTAATGATAC CAGGCCTAAATGTGGATTTTGCCATGTAGGGGAGGAAGAAAATGAAGCAAGAGGGAAATTGCATATATTT AATGCCAAGAAGGCAGCCGCACATTACAAGTGCATGTTGTTTTCTTCTGGCACTGTTCAGCTCACAACAA CATCAAGAGCAGAATTTGGAGATTTCGATATTAAAACTGTACTTCAGGAGATTAAGCGAGGAAAAAGGAT GAAATGTACACTTTGCAGTCAGCCTGGTGCTACTATTGGATGCGAAATAAAAGCCTGTGTTAAAACCTAC CATTACCACTGTGGTGTACAAGACAAGGCAAAATACATTGAAAATATGTCACGAGGAATTTACAAACTCT ATTGTAAAAACCATAGTGGAAATGATGAGCGAGATGAAGAAGATGAGGAGCGAGAGAGTAAAAGCCGTGG AAGAGTAGCGATTGATCAGCAGCTAACCCAGCAGCAGCTTAATGGAAACTAGGTTCGTGGGACAGAGAGA GTTAGAAAACTGAGAAAAAACAACTATAACTCCTGTGCTTCTTTTCCACTTTTTTCTAAAATAAAGGGTT TGTAACTTTTTACTGCCCGACTCTTAGATCCTTCATTGAACTGCCTAAATCACCTTGTTAGTTTTCTGAG CCTTCAGTAGACGGCCGAATTTGACATATTGATATTACGTAGCTGTAGTTTGCAGTTTCTGGGAAGCAGT CAAAACACTATTAGCCCTGTGTATACTGTCAGCAGTTAAAACAGACATTGAATGAAGTAAACTGTATTTC TGGACAGAACAAAGTTGTACTGTTTAGAGTATGTAAGTTTGTGTAGTAGATCCACGCTCAAAGGAAACCA GAAGCAAGTTGTACTCCACCTTGCAAACATGCCTCTGGTAGCAACACCTGGCAATTTGGGCAATGGAAAC GACAAATGAAGAGACTCTTCTTTTTGTCTTGGCTTTGACAGCACATGCTCTAACCATTTCTTTCACGAAG CGTGCTGAAGCCTACCTGTGGTCCTTTTTAGAGTTATTCTCACTGAGCAGCATGGACTCTGGGCGACTGG TGATGATAAGCTCTGAGCTCCAGTGCTTCTGCCAAAACTCTGGACAGTACACCTTTCTGTGCTGTACAGA GAGCTTCTGGCAAAATAATGGAATCGTATCAAGATCAAAATAATGTTTGGCAACATCAGATTAAACCTCT TTGAAATTCAAGAGGTCAGCAATGCGCTAAAGGACTTTGGACATCATGTCTGAAACCAGCTTCTCTCAAG TGCCCTGTTGTGTGGAACACTTCACATATTGGCCCAGCATATATAAGGAGGTCTTATATGCTAACATTTT TTTTAAAGGAACCATCTTTGTGCAAAACAGTATTTCTTTTTGACTCTGTGACCACTATTTTAGAAACATT ATTTAATGCTTTCAGTCATCAGTTTGCTTACAGATTGAATAAGCTTTTGTGCCCTGTTTTTCTTCGAGGG CTCGGAAGTGTTTTCTCAATCACCAGATTTTTAGAAAGGCTTTCTTTCCACTGGCATTTCTCACTCGTGA CACACATCCACTTACCTGCTGAAGCACATTTACATATTTCTTTATGGCAAAACCAAAAAAGTTAGTTGTG GTCTGCCTAATATTACCATAGCACCTCAGTATGAAAGGTAAGGATTCTGAGTTCCACATCATTAGTTAAC CTCCCATAGTGAATCAGATTTGCAAATAAGAAATAACGTACTAACTTGACTGTACATCCATCTATTCTGT AGCTGGTAGAACAGCGTTTTGAAATGTAACAGGTGGAAAATTACACTGTGCTTATTGACTGAATTTTTAA ACTGCTTGTCCCTTAAGATCATGTTTGTTAAGGTGTGTATTTACACATTTACTTAAATCAAGGGACTCAA TGCTTGCTTTCTTTTAACCATCTTTTATTGTTATTTTAGAAGGAAACTAGCTTTAGTGGATTGCCTTGTG CGTCTTCTCCTTTCGCTCTTAATATGCCATGATGTGTATGTGATTAATTTTCAAGGTTCATCATGACTTG AAGTGCAAGGACAGATCTCAGTTTGTTTACCAAGCGATGCGACTTTTCCCAAAGGATCTGTACTTTATTT CCTTATAACAGCTTGAAAACCATTCTTTTTTAAATCTTTAAATCACTGTGTCTAGGTAATTTTTCCATTG TGTGCCATAGAACTTACCCATGGAGAAATGGAGCCCCTTCATTTTTGGACAACTACTGTAATGATTTTTT TTAAGAAAAATGGAAGGTGCCAGGGGTAATTATGGCCAGTCAATAATTATATAAAGGACTTCAAATACTA TAGCCGTCAGTTGATGGATTTGATATGTAGTACATGTATGATTTTGTATGGGTTTCTTCAGCCCTTTCTC TGCCACTAGCAACCAGAAAAGCACTTTACCTTTTGGTTGGTTAGATAAGTGGCTAACTACCTGTTTTTCT CACTGTGGTGTGATTGGCTAGATAACCTTCATTTAAAGTTGAAATGTAAACTGAAGTCACATTAAACACT CACGTTTGGCTGTAAACTTCCAATAGTTTGATTCTTTGTTCCTGTTTTCATTCTATGCTGAGTAAAAGTG CCTTACAATGTAAAAATTGTACAGTACTTACGTTCCCAAGTAGCAGCTTCACCATCTGGCTAGGCGTCAC ATTGTGGTAGGAGTATTAGATCAATAGACCTCAGCAGTGTATTTACTGTACAACTCTTAAGTCCTTAACT GTAGTTTTAATAAGCATGACATTATTTGGCAAATATATGGCATTTACATCATTTCCAAAAATACTGCCAT TGCTGTCTACTGCGTATTTTTAGCCTGAAATTTTGATGCGTCTTTTTTTCTTGTTTTTTTTTCTTTATTT CTTTTGTTCCCTTTTGTCATTATTAATATTAAACCGTGTAATCTTTGCAAGCGTATATTGAAGATTATTC TGGAGCATTTATTGCCTTACCAGAAATGTTAGTAAGAAATGTTCTTTAGAGTAGAAGGGTAGACTTGAGT TTCTATACTTTAATAAGACCTCTTTGTTCCTGGGGGGAGGGGGGAGTTTTGCTTTCATCTCATTGTTTTA AAGCCCACACAAGAAATTAATTTCATGTCAAGGATCAGCAGTTTTCAAACTTGAGGTGGTTCTTAGTACA CACGACTCAAGATACTAACAGAGTAACATGATCTACAGATCTGCTATTGAATCGAATCTATGTACTTGAA CAAATGTGTGAATCTCAAATATCTCAAAGCAAAAGTGTTGTAGAATGTTGTTGCTTTTTGAAAAAGCACT GAAGTTAGACCAAGGCACAGTTTTGTTTTAGCAGACATTTAAGTTAATTGTAAAGATAAGGAATGCATCA TGGGTCAAAAATCAAACATTCCTCAATGTTATGTATATTTTGTTCCTGTTATATTGGCTTTATTTTCAAA ATTGTAGTTTGTAGTATTAGTTTCCTTTTAATGGTATTCTTGCATATACTATTCATTCAATAAATGAGTT CTGGATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6410361 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6410361 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19682 same embryo
	EMAGE:19683 same embryo
	EMAGE:19684 same embryo
	EMAGE:19685 same embryo
	EurExpress:euxassay_005159 same experiment
	MGI:4827188 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source