EMAGE mouse gene expression spatial database

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EMAGE:19705

Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B ( MGI:2151841)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006167. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006167_01	euxassay_006167_02	euxassay_006167_03	euxassay_006167_04

euxassay_006167_05	euxassay_006167_06	euxassay_006167_07	euxassay_006167_08	euxassay_006167_09

euxassay_006167_10	euxassay_006167_11	euxassay_006167_12	euxassay_006167_13	euxassay_006167_14

euxassay_006167_15	euxassay_006167_16	euxassay_006167_17	euxassay_006167_18	euxassay_006167_19

euxassay_006167_20	euxassay_006167_21	euxassay_006167_22	euxassay_006167_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19705_wholemount_strong.wlz

19705_wholemount_moderate.wlz

19705_wholemount_weak.wlz

19705_wholemount_possible.wlz

19705_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19705_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

spotted

moderate expression: see section 01 02 04 08 09 11 12 13 14 16 17 18 19 20 21 22 23 weak expression: see section 03

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thymus primordium

moderate

spotted

moderate expression: see section 08 09 10 11 12

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

pons mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

spinal cord mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

heart ventricle

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

stomach

moderate

spotted

moderate expression: see section 01 02 03 04

midgut

moderate

spotted

moderate expression: see section 09 13 weak expression: see section 10 11 12

metanephros

moderate

spotted

moderate expression: see section 05 06 07 08 13 14 15 16 17

lung

moderate

spotted

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8874
Entity Detected:	Ppp1r16b, protein phosphatase 1, regulatory (inhibitor) subunit 16B ( MGI:2151841)
Sequence:	sense strand is shown >T8874 CGGTGGCTCGCTACCCAGCCGAGCTGGCTCTGTCGCCGCACCACCAGCTGCGGCTCTGGGACCTGCGGAT CGAGCCGGGGCCGGAGCCGCCCGGGGTAGGGCTCGAGAGAGCGGGCCCCACCATGAGGCTCCAGCCCCTT GGGAGCCCCTGTGCTCCCCTGGCCCCGGGCTCACCGCGCTAGCCCCAGGCAGAGCAAGGGCAGGCGCGGG GCCATGGCCAGCCACGTGGACCTGCTCACAGAGCTGCAGCTGCTGGAGAAGGTGCCTACGCTGGAGCGCC TGCGCGCAGCCCAGAAGCGCCGGGCTCAGCAGCTGAAGAAGTGGGCACAGTACGAGCAGGACCTGCTTCA TCGCAAGCGCAAGCATGAGCGGAAGCGCAGCACGGGTGGACGTCGCAAGAAGGTGTCCTTCGAGGCCAGT GTAGCGTTGCTGGAAGCCTCACTGAGGAATGACGCCGAAGAAGTTCGCTACTTCCTGAAGAACAAGGTTA GCCCTGATTTGTGCAATGAGGATGGACTTACAGCCTTGCACCAGTGCTGCATTGACAACTTTGAAGAGAT TGTCAAGCTGCTGCTGTCCCACGGTGCGAACGTGAATGCCAAGGACAACGAGCTGTGGACGCCCTTGCAC GCGGCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCTGACTTGCTTGCTG TCAACTCTGATGGAAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGGATGTCATCGAGACCTG CATGGCATACCAAGGCATCACACAGGAGAAAATCAACGAGATGCGAGCAGCCCCTGAGCAGAAGATGATT TCAGACATCCACTGCATGATAGCAGCGGGCCAGGATCTCGACTGGATCGATGGCCAGGGTGCCACACTGC TACACATAGCTGGAGCCAATGGATATCTGCGAGCAGCCGAGCTCCTCCTGGACCATGGAGTGCGTGTGGA TGTGAAGGACTGGGATGGCTGGGAGCCTCTGCATGCAGCAGCCTTCTGGGGACAGATGCCAATGGCAGAG CTGCTGGTGTCCCATGGAGCTAGTCTCAGTGCCAGGACATCCATGGATGAGATGCCAATAGACCTGTGTG AGGAGGAGGAATTCAAGGTCCTGCTGTTGGAGCTGAAACACAAGCATGATGTGATCATGAAGTCGCAGCT GAGGCACAAGTCGTCCCTGAGCCGGAGGACATCCAGCGCGGGCAGCCGTGGGAAGGTGGTGCGTCGAGCC AGCCTGTCAGACAGAACCAATCTATACCGGAAAGAATATGAGGGCGAGGCCATCTTGTGGCAGCAGCGGA GTGCAGCTGAGGACCAGCGGACTTCAACCTACAATGGAGACATCAGGGAGACCAGGACAGACCAGGAGAA CAAGGACCCGAACCCCAGGCTGGAGAAGCCCGTGCTGCTCTCAGAATTCTCTACCAAGATCTCACGGGGA GAACTGGATGGGCCTGTGGAGAATGGCCTCCGGGCTCCTGTCAGCACTTACCAGTATGCGCTGGCCAATG GGGACATATGGAAGATGCACGAGATGCCTGACTACAGCATGGCCTACGGCAACCCTGGCGTGGCTGATGT CCCCCCACCCTGGAGTGGCTTCAAGGAGCAGAGCCCCCAAACGCTCCTGGAGCTGAAGAGGCAGAGGGCC GCAGCCAAGCTGCTCAGTCACCCATTCTTAAGCACCCACCTGGGCAGCAGCGTGGCCAGGTCGGGGGAGA GCAGCAGCGAAGGCAAGGCCCCCTTGATTGGAGGCAGAACTTCCCCCTACAGCAGCAACGGGACCTCGGT GTATTATACAGTCACCAGCGGAGATCCCCCGCTCTTGAAGTTCAAGGCCCCCATGGAGGAGATGGAGGAG AAAGTTCACGGCTGCTGCCGGATCTCCTAGTGTCCTTGAACTGGAGGAGAGAGCTGCCTCGGAGAGGGGC CCCTGGAATCCAAAGCCGGTCCAACAGCTCTGGCTGGCGAGGTGTCGGGGCAGCCGGGGAGAGGTGGGAT CTGCTTTCTAGAGGAATGCTGACCTTACCTCATGCCCAGCCGCCCATAGCATCTCCACTCCCTTCTGTGC TGCTTCCTTGCCTCACTCAATGCCTGCACCTGAAGTATAGCAGGCCGACTCACTCTGCAGTCTGATTCAG GAAGGGTGGGCCTGGGATCTCACTTCTGCACTTGGTAAAGGCTTCTTAGGACCAGGAATATCAACTCCCT ACACACACACACACACACACACACACACACACACACACACACACACACACACATACTCAATTTAGTGTAT AAGGATGTTACACCTGAGCTCTTGGGAAACAAATGAGGCTAAGAACTTGTGGGTTATCCCCAGAAAATGA ATGAGTTCAAGACAGTCTTGGCACCACTGCCAACTGGCTTCCACTGGGGAGAGGCCTGCTCAGCGGAGAC CCACAGCTCTGAAGTCATACCAGGCTGGGTCACTAGCACACCTAGCAAGAGACAGGAAGTCCTATGGGTT CAGGGAATCCGGGAATCCATACGGATCAGGTTAGTGGGCCCAGGCCTGCTCCCCACAGCCTTGCTGAGAG CTGTTCTCATACATATGTCAATAAGAAAGTGGAGAGTGTTCAAAGGTCAAACAGGAAAGCCAGGCCATCC ATGCTTCTGTGGGAGCACAGGAGTCGGGTTGGGTGCAGCACATTGGTATTTTCCAAACTGTGTTTGGTAA AAGCGTTCTTTCAGGCTGTAAGATCCCAGGTGGGAGGTTCCTGAATTTGCCTTCTCCCAAGATAGTGTCA CACTTGGGTTTTATTGTCTGGTATTTGAGAAATGAATTTCATCAGGAGCTTCCGTGGGGCTAGGGCTCCC AGGGAGAAGGTGAACGCATTGCTGAGAACACCATGGTTAACATTGACATAACAGCAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6414610 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19704 same embryo
	EMAGE:19702 same embryo
	EMAGE:19701 same embryo
	EMAGE:19703 same embryo
	EurExpress:euxassay_006167 same experiment
	MGI:4827371 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source