EMAGE mouse gene expression spatial database

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EMAGE:19783

Tars threonyl-tRNA synthetase ( MGI:106314)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006234. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006234_01	euxassay_006234_02	euxassay_006234_03	euxassay_006234_04

euxassay_006234_05	euxassay_006234_06	euxassay_006234_07	euxassay_006234_08	euxassay_006234_09

euxassay_006234_10	euxassay_006234_11	euxassay_006234_12	euxassay_006234_13	euxassay_006234_14

euxassay_006234_15	euxassay_006234_16	euxassay_006234_17	euxassay_006234_18	euxassay_006234_19

euxassay_006234_20	euxassay_006234_21	euxassay_006234_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19783_wholemount_strong.wlz

19783_wholemount_moderate.wlz

19783_wholemount_weak.wlz

19783_wholemount_possible.wlz

19783_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19783_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 12 13 14 15 16 17 18 19 20 21 22

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 07 08 21 22 weak expression: see section 09 10

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

liver right lobe

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22

renal cortex

moderate

regional

moderate expression: see section 05 06 07 08 09 14 15 16 17 18

orbito-sphenoid

strong

regional

strong expression: see section 05 moderate expression: see section 01 03 04 06 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T635
Entity Detected:	Tars, threonyl-tRNA synthetase ( MGI:106314)
Sequence:	sense strand is shown >T635 TCCTCGAGNCTGTTGGCCTACTGGAAACACTTGAAAACAGTTTGAATGAGTTCGGTGAGAAGTGGGAACT AAATCCTGGAGATGGAGCCTTCTATGGGCCAAAGATTGACATACAGATAAAAGATGCCATCGGTCGCTAC CACCAGTGTGCAACCATCCAGCTGGACTTTCAGTTGCCCATCAGGTTTAACCTTACTTACGTCAGCCACG ATGGCGATGATAAGAAAAGGCCAGTGATTGTTCACAGAGCCATCCTGGGGTCGGTGGAAAGAATGATTGC CATCCTCACAGAAAACTATGGGGGCAAATGGCCTTTCTGGCTCTCTCCTCGCCAGGTGATGGTGGTCCCA GTGGGACCGACATGTGATGAATATGCCCAAAAGGTACGGCAACAATTCCATGATGCTAAATTCATGGCGG ACACTGACCTGGATCCAGGCTGTACCTTGAATAAGAAGATCAGAAACGCACAGTTAGCACAGTATAACTT CATCCTTGTTGTTGGTGAAAAAGAGAAAGCCAGTGGCACGGTGAACATCCGCACGAGA
Notes:	The probe template was PCR amplified from IMAGE:1886765 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1886765 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19782 same embryo
	EMAGE:19781 same embryo
	EMAGE:19780 same embryo
	EMAGE:19779 same embryo
	EMAGE:19778 same embryo
	EurExpress:euxassay_006234 same experiment
	MGI:4828601 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source