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EMAGE:19788

Flrt3 fibronectin leucine rich transmembrane protein 3 ( MGI:1918686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788
"Pseudo-wholemount" of euxassay_006295. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006295_01 euxassay_006295_02 euxassay_006295_03 euxassay_006295_04
EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788
euxassay_006295_05 euxassay_006295_06 euxassay_006295_07 euxassay_006295_08 euxassay_006295_09
EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788
euxassay_006295_10 euxassay_006295_11 euxassay_006295_12 euxassay_006295_13 euxassay_006295_14
EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788
euxassay_006295_15 euxassay_006295_16 euxassay_006295_17 euxassay_006295_18 euxassay_006295_19
EMAGE:19788 EMAGE:19788 EMAGE:19788 EMAGE:19788
euxassay_006295_20 euxassay_006295_21 euxassay_006295_22 euxassay_006295_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19788Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19788_wholemount_strong.wlz
19788_wholemount_moderate.wlz
19788_wholemount_weak.wlz
19788_wholemount_possible.wlz
19788_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19788_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 09 10 18 19 20
pituitary gland
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
physiological umbilical hernia
moderate moderate
regionalmoderate expression: see section 14 15
vibrissa
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 21 22 23
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 13 14 moderate expression: see section 08 09 10 11 12 15 16 17 18
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 13 14 moderate expression: see section 12 15
telencephalon mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 17 18 19 20 21 22 23
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 04 05 06 08 09 10 11 12 13 17 18 19 20 21 22 23 moderate expression: see section 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 13 14 15 16 17 weak expression: see section 10 11 12
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 weak expression: see section 18 19
pons mantle layer
moderate moderate
regionalmoderate expression: see section 06 08 09 10 11 12 13 14 15 weak expression: see section 16 17 18
pons marginal layer
moderate moderate
regionalmoderate expression: see section 07
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 weak expression: see section 17
midbrain mantle layer
strong strong
regionalstrong expression: see section 13 14 moderate expression: see section 05 06 07 08 09 10 11 12 15 16 17 18
midbrain ventricular layer
strong strong
regionalstrong expression: see section 08 09 12 13 14 moderate expression: see section 10 11 15 16
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 09 13 14 15 weak expression: see section 08 10 11 12 16
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 19 20 23 moderate expression: see section 21 22
eyelid
moderate moderate
regionalmoderate expression: see section 02 03 04 05
lower lip
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 10 13 14 15 16 18 19 20 21
mandible
strong strong
regionalstrong expression: see section 04 05 23 moderate expression: see section 06 weak expression: see section 07 21 22
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 13 14 15 18
lower jaw molar
moderate moderate
regionalmoderate expression: see section 09 20 weak expression: see section 19
upper lip
weak weak
regionalweak expression: see section 09 10 13 14 15 16 18 19 20 21 22
maxilla
strong strong
regionalstrong expression: see section 05 moderate expression: see section 06
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 14 17 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 09 20 weak expression: see section 19
bladder
weak weak
regionalweak expression: see section 13 14 15
metanephros
weak weak
regionalweak expression: see section 07 08 09 10 11 17 18 19 20
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 14 15 16 17
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8469
Entity Detected:Flrt3, fibronectin leucine rich transmembrane protein 3 ( MGI:1918686)
Sequence:sense strand is shown

>T8469
CGCGCAGTCAACTGGATGCTCTCGCGCGCAGTCCTCAGCGCAGGAGTGATCTGAAGCAGGACCGCTCAGG
CTGCAGCTGCCGTGGCTTTGTGTGCACTGGATGCCGCGCTCCGGAGACGGGGGAGGGTTTTTACTTCAAC
TCACAGGCAATGGAATTACAGCTGTAGCAGCAGGGTATAAAGGATTGCTTTTTCCTCGTCTTCCTGGAGG
TGCTCAGTCCTGGCCCATTTTAAGGACGAGAAATATAAAGGGAATTTAGTGTGCTTCCTTCTCTTTCCAT
GAAGACTGCATGCACTGTGCCCTTTGCTTCTTAAAAGAGACTCCACCCACTCCAGTAGACCGGGGACTAA
AACAGAAATTCTGAGAAAGCAGCAAGAAGCAGAAGAAATAGCTATTTCACAGCAGTAACAGAAGCTACCT
GCTATAATAAAGACCTCAACACTGCTGACCATGATCAGCCCAGCCTGGAGCCTCTTCCTCATCGGGACTA
AAATTGGGCTGTTCTTCCAAGTGGCACCTCTGTCAGTTGTGGCTAAATCCTGTCCATCTGTATGTCGCTG
TGACGCAGGCTTCATTTACTGTAACGATCGCTCTCTGACATCCATTCCAGTGGGAATTCCGGAGGATGCT
ACAACACTCTACCTTCAGAACAACCAAATAAACAATGTTGGGATTCCTTCCGATTTGAAGAACTTGCTGA
AAGTACAAAGAATATACCTATACCACAACAGTTTAGATGAATTCCCTACCAACCTTCCAAAGTATGTCAA
AGAGTTACATTTGCAAGAGAATAACATAAGGACTATCACCTATGATTCACTTTCGAAAATTCCGTATCTG
GAAGAGTTACACTTGGATGATAACTCAGTCTCGGCTGTTAGCATCGAAGAGGGAGCATTTCGAGACAGTA
ACTATCTGCGGCTGCTTTTTCTGTCCCGTAACCACCTTAGCACAATCCCGGGGGGCTTGCCCAGGACTAT
TGAGGAATTACGCCTGGATGACAATCGCATATCAACGATCTCTTCCCCATCACTTCATGGTCTCACAAGC
CTGAAACGCCTGGTTTTAGATGGAAACTTGTTGAACAACCATGGTTTGGGTGACAAAGTTTTCTTCAACT
TAGTAAACTTAACAGAGCTGTCCCTGGTGAGGAATTCCTTGACAGCAGCGCCAGTGAACCTTCCCGGCAC
AAGCCTGAGGAAGCTTTACCTTCAAGACAACCATATCAACCGGGTACCCCCAAATGCTTTTTCTTATTTA
AGGCAGCTGTATCGACTCGATATGTCTAATAATAACCTAAGCAATTTACCTCAGGGTATCTTTGATGATT
TGGACAATATAACCCAACTGATTCTTCGCAACAATCCTTGGTATTGTGGATGCAAGATGAAATGGGTACG
AGACTGGTTACAGTCGCTACCGGTGAAGGTCAATGTGCGTGGGCTCATGTGCCAAGCCCCAGAAAAGGTC
CGTGGAATGGCTATCAAGGACCTCAGTGCAGAACTGTTTGATTGTAAAGACAGTGGGATTGTGAGCACCA
TTCAGATAACCACTGCAATACCCAACACAGCATATCCTGCTCAAGGACAGTGGCCAGCTCCTGTGACCAA
ACAACCAGATATTAAAAACCCCAAGCTCATTAAGGATCAGCGAACTACAGGCAGCCCCTCACGGAAAACA
ATTTTAATTACTGTGAAATCTGTCACCCCTGACACAATCCACATATCCTGGAGACTTGCTCTGCCTATGA
CTGCTCTGCGACTCAGCTGGCTTAAACTGGGCCATAGCCCAGCCTTTGGATCTATAACAGAAACAATCGT
AACAGGAGAACGCAGTGAATACTTGGTCACCGCCCTAGAACCTGAATCACCCTATAGAGTATGCATGGTT
CCCATGGAAACCAGTAACCTTTACCTGTTTGATGAAACACCTGTTTGTATTGAGACCCAAACTGCCCCTC
TTCGAATGTACAACCCCACAACCACCCTCAATCGAGAGCAAGAGAAAGAACCTTACAAAAATCCAAATTT
ACCTTTGGCTGCCATCATTGGTGGGGCTGTGGCCCTGGTAAGCATCGCCCTCCTTGCTTTGGTGTGTTGG
TATGTGCATAGGAACGGGTCACTGTTTTCACGGAACTGTGCGTACAGCAAAGGGCGGAGGAGAAAGGATG
ACTATGCAGAAGCCGGTACTAAGAAAGACAACTCCATCCTGGAAATCAGGGAAACTTCTTTCCAGATGCT
ACCGATAAGCAATGAACCCATCTCCAAGGAGGAGTTTGTAATACACACCATATTTCCTCCGAATGGGATG
AATCTGTACAAGAACAACCTCAGTGAGAGCAGTAGTAACCGGAGCTACAGAGACAGTGGCATCCCAGACT
CGGACCACTCACACTCATGATGCAAGGAGGTCCCACACCAGACTGTTCCGGGTTTTTTTTTAAAAAACCT
AAGAAAGGTGATGGTAGGAACTCTGTTCTACTGCAAAACACTGGAAAAGAGACTGAGAGAAGCAATGTAC
TGTACATTTGCCATATAATTTATATTTAAGAACTTTTTATTAAAAGTTTCAGATTTCAGGTTGCTGCTGC
GGTTGATGTAGTGGGGATGCCTGAACACAATTCTATATTTTAGTATTTTTTAGTAATTTGTACTGTATTT
TCCTTGCAGATATTGAAGTTATAAACCATTTACTTTGTGTTCTACTGAGTAAGATGACTTGTTGACTGTG
AAAGTGAATTTTCCCGCTGTGTTGAACAATCAGGACTGCGTTCACATGAGACCCTTGTAGTATAAGCACA
GGCCGTTTTTCACTTTGGTATTAATACAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5702881 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5702881 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19786 same embryo
 EMAGE:19785 same embryo
 EMAGE:19784 same embryo
 EMAGE:19789 same embryo
 EMAGE:19787 same embryo
 EurExpress:euxassay_006295 same experiment
 MGI:4824881 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS