EMAGE mouse gene expression spatial database

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EMAGE:19793

Zcchc12 zinc finger, CCHC domain containing 12 ( MGI:1919943)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006307. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006307_01	euxassay_006307_02	euxassay_006307_03	euxassay_006307_04

euxassay_006307_05	euxassay_006307_06	euxassay_006307_07	euxassay_006307_08	euxassay_006307_09

euxassay_006307_10	euxassay_006307_11	euxassay_006307_12	euxassay_006307_13	euxassay_006307_14

euxassay_006307_15	euxassay_006307_16	euxassay_006307_17	euxassay_006307_18	euxassay_006307_19

euxassay_006307_20	euxassay_006307_21	euxassay_006307_22	euxassay_006307_23	euxassay_006307_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19793_wholemount_strong.wlz

19793_wholemount_moderate.wlz

19793_wholemount_weak.wlz

19793_wholemount_possible.wlz

19793_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19793_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

anterior abdominal wall musculature

strong

regional

strong expression: see section 09 10 11 12 13 16 17 18 moderate expression: see section 01 02 03 04 05 06 07 08 14 15 19 20 21 22 23 24

pelvic girdle musculature

moderate

regional

moderate expression: see section 19 20 weak expression: see section 04 05 06 07 08 09

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall marginal layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

cerebral cortex marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24 moderate expression: see section 01 02

telencephalon marginal layer

strong

regional

strong expression: see section 15

olfactory cortex mantle layer

strong

regional

strong expression: see section 14 17

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 14 17 18 19 20

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 09 10 11 12 13 14 15 16 17 18

midbrain marginal layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 06 17

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23 24

vagus x ganglion

moderate

homogeneous

moderate expression: see section 07 17

trigeminal v nerve

strong

regional

strong expression: see section 10 11 17 18 19

spinal cord mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

spinal cord marginal layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 07 08 12 13 14

cervical ganglion

strong

regional

strong expression: see section 07 08 16

thoracic ganglion

strong

regional

strong expression: see section 08 13 14 moderate expression: see section 09 11 12

neural retina

weak

regional

weak expression: see section 02 03 04 05 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 14 15 17 18 weak expression: see section 11 12 13 19 20 21

esophagus

moderate

spotted

moderate expression: see section 10 11 12

tongue

strong

spotted

strong expression: see section 12 13 15 16

stomach

strong

spotted

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 08 09 10 11

midgut

moderate

spotted

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8448
Entity Detected:	Zcchc12, zinc finger, CCHC domain containing 12 ( MGI:1919943)
Sequence:	sense strand is shown >T8448 GCTGCTGATAAAAGCTTTGCAGCTGCCTTGGAAACTGCGCTATTCGAATCCGGTTACCTGTGGCAGCCCA TCAAAGCGGAATCTGAACTTGAATTTGGTGCAGCTGGTTGTAGAGCTGGTCTTGAGCATCAGGATCTGAG ACCCCCAGTCGATCGAGGTCCATCAACTGGACCCTGGAGGCAGGCTTTCTCACGGGAGGGAGGACACAGG TTGCAGAAGCTGGAAATACTCCAGTTTCCCAGCTGATCCCCCTACTGAGATTGGAGACTGCTGCACTGCT GAAGATAAAGCGTTTTTGTTTTGTTTCTAAAACGCCTTGAACTTCGTGTATTAAGATATCCTATCACTGC TGTCAGACATGGCTAGCATCCTTTCACGTTTGGGTAGCAGCCGGGGGCAGAACTCACCCCTGCCACCTTG GGCTCATTCCATGCTGAGATCCCTGGGGAGGAGTCTTGGCCCTTTAATGGCCAGCATGGCAGAGAGAAAC ATGAGGTTGTTCTCTGGGAGGGCAGAGCCAGCCCAGGGGGAAGAAACCTTTGAAAACTGGCTGAGCCAAG TAACTGGGGTGCTGCCTGATTGGCATATGCCTGAGGAGGAAAAGGTCAGACGTCTAATGAGAACCCTTAG AGGCCCTGCCCGCGAGGTCATGCGTTTGCTCCAAGCTGCCAATCCTGGCCTAGATGTAGAGGATTTTTTG CGGGCGATGAAACTGGTCTTTGGGGAGTCTGAGAGCAGTGTGACAGCCCATAGTAAATTTGTTAACACTG TTCAGGAACATGGAGAGAAACCATCCCTGTATGTGATCCGTTTAGAGGTGCAGCTGCAGAATGCTATCCA GGCAGGGGTGTTTGCTGAGAGAGAGGCAAACCAGGCTCGCCTGCATCAGCTCCTGGTAGGAGCTGAGATG AGTACAGACCTTCGATTCAGGCTTAAGAATCTTCTCCGGGTATATGCAAATGAGCCAGAGCGCCTTCCCA ATTTCCTAGAGTTAATCAGGATGATAAGGGAGGAAGAGGAATGGGAGGAGACTTTTATTAATCCAAAGCG GCCCAGAAGAGCCGAGTCAGTGATGGAGAGGGCACTCAGCCCTATGGCATTTCAGAGCTCCCCACCAATA ATGATCAGCAGTATCGACTGCAATGTGATCGAGATAGATGACTCTCCTGATGATTCGGATGAGGATGTGA TCTTGGTGGAGCCTGAGGACCCACCACTGCCATCCTCAAGTGCCTCTTCCTTCCTAGGCAGGGCTGTATC TGAGGACCAAGTGCTGGTCATTGAGTCCCCAAACATTTTTGAGATCCAGGCTCCTTCCACTAGCAGTGGT GCTGGGAGAAAGAACAATCGGGGTTTTGGGGAGCTGCGTAGAGCCAGGAAGCGCAAACATACGATCCATT GTTCCCACTGTGGTGAGGAGGGCCACTCAAAAGAAACCTGTGACAATGAGAGTGACAAGGGCCAGGTTTT TGAGAATCTGATCATCACCCTGCAGGAGCTGACACATGCAGAGGAGAGGGCAAGGGGGGCCCCTGGTGAA CCCATTGGCCTATCTGAACCACAGTAAGATGCGACTCCCCAGCCTTAAATTAACCCTTACCCGTATTAAA TGTTTAACGGTGGAAAAGTTGTGGGGACAGATTTCTAATTGCATGAACAAATCCACAAAGTGGCTTTCTT TGGGGGTGGAGAAAGGGTCCCGAATACCAGTACAGTAAAGAGAGGTGGCCTGACCTATGATCGTGTTCCC ATTCCCCACTGCTTAACAGTCTATTTTCTGTGTGAACCTCTCTCCTTGGTGGCTTTGTTCTCTTTGTGAA AGTGGTATTGTTCATTGTTCAATCTTCAAGTAAGAAAATTATTAAAAAGTACAAATTACCCAGACCTCTC TACCTTGTGAAACAATTGTCAGCCCTTTGGTGCCTATCCTTCTAAATATTTCTCTATATCTGTGTTCCTA GATTAGAAATATGTATAGACGAAAGTGATCAAATAGAAGTGTTGTTCTATATGCTGTATTTTTTCACCAA AACGTATGTTGTGGCCTTCTTTGTCAATAAATATATACATATATGTCAGCATCTAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5701407 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5701407 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19792 same embryo
	EMAGE:19794 same embryo
	EurExpress:euxassay_006307 same experiment
	MGI:4829270 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source