EMAGE mouse gene expression spatial database

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EMAGE:20085

Bcl7a B-cell CLL/lymphoma 7A ( MGI:1924295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009092_01	euxassay_009092_02	euxassay_009092_03	euxassay_009092_04

euxassay_009092_05	euxassay_009092_06	euxassay_009092_07	euxassay_009092_08	euxassay_009092_09

euxassay_009092_10	euxassay_009092_11	euxassay_009092_12	euxassay_009092_13	euxassay_009092_14

euxassay_009092_15	euxassay_009092_16	euxassay_009092_17	euxassay_009092_18	euxassay_009092_19

euxassay_009092_20	euxassay_009092_21	euxassay_009092_22	euxassay_009092_23	euxassay_009092_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20085_wholemount_strong.wlz

20085_wholemount_moderate.wlz

20085_wholemount_weak.wlz

20085_wholemount_possible.wlz

20085_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20085_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 15 weak expression: see section 11 12 13 14

thyroid gland

moderate

regional

moderate expression: see section 10 11 15

diencephalon

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

vibrissa

weak

regional

weak expression: see section 06 07 08 21

telencephalon

strong

homogeneous

strong expression: see section 01 02 03 20 21 22 23 moderate expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 24

medulla oblongata

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

metencephalon

strong

homogeneous

strong expression: see section 20 21 moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 17 18 19 20

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 09 17 weak expression: see section 07 08 10 11 12 16

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05 22 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

vomeronasal organ

strong

regional

strong expression: see section 12 13 15

lower jaw incisor

weak

regional

weak expression: see section 12 13 16 17

lower jaw molar

weak

regional

weak expression: see section 08 09 19

upper jaw incisor

weak

regional

weak expression: see section 12 13 16

upper jaw molar

weak

regional

weak expression: see section 08 19

metanephros

moderate

regional

moderate expression: see section 09 10 11 12 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2407
Entity Detected:	Bcl7a, B-cell CLL/lymphoma 7A ( MGI:1924295)
Sequence:	sense strand is shown >T2407 TGGCCTCGAGCCAGATTCGGCACGAGGGTTTTGTTTCGTTTGCTAATTGGTGCATATTCAGGTACCACCT TTTGACGTGTGGATCTTTCTCCAAACCACCACAAGAAGTGTCTGCCGGGCTCCGTTTTCTAAGAGTTTCT GAGGGGACAGCTCCCATTTCTTTTTTTGGTTTCAAGGGAGCTGTCTATTTCCTATACTTCAAGAAGAATC AAAATGTTCCTGAATTTTAAATACCTCATGCAAAAATATCTCCTGAAATAAGGGAAAAAAAAAAAACTTT GAAAAATCGTAATGTTGAAGTTAGCGATGCTAAAATGTTTCTGTCTTAAAAAACAAAAAAATTGTTGTAA TACTTAGCGATTTTGCCCCTCAGGCGGTCAGTTCTGTCCAGAACTGTGTTCTGCGTCTTGGCCCGGAAGC AACCGGATGCATGACCTCTGAACGGATCTCAAGGCCAAGGCATCTTTACCTCCAGATTCTAGAGTTAGGG CAACAACAGTTTTCTTTTGCAGCAAAACTCCGTTCTGGTGAAAGATGAATTTGGATA
Notes:	The probe template was PCR amplified from IMAGE:1225594 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1225594 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20086 same embryo
	EMAGE:20084 same embryo
	EMAGE:20083 same embryo
	EurExpress:euxassay_009092 same experiment
	MGI:4823452 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source