EMAGE mouse gene expression spatial database

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EMAGE:20187

2010107G23Rik RIKEN cDNA 2010107G23 gene ( MGI:1917144)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012442. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012442_01	euxassay_012442_02	euxassay_012442_03	euxassay_012442_04

euxassay_012442_05	euxassay_012442_06	euxassay_012442_07	euxassay_012442_08	euxassay_012442_09

euxassay_012442_10	euxassay_012442_11	euxassay_012442_12	euxassay_012442_13	euxassay_012442_14

euxassay_012442_15	euxassay_012442_16	euxassay_012442_17	euxassay_012442_18	euxassay_012442_19

euxassay_012442_20	euxassay_012442_21	euxassay_012442_22	euxassay_012442_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20187_wholemount_strong.wlz

20187_wholemount_moderate.wlz

20187_wholemount_weak.wlz

20187_wholemount_possible.wlz

20187_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20187_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 17 18

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17 18

cerebral cortex mantle layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 04 05 07 12 14 15 16 18 19 20 21 22 23

olfactory cortex mantle layer

weak

regional

weak expression: see section 10 11 12 15 16

midbrain mantle layer

weak

regional

weak expression: see section 09 10 11 13 15 16 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 17 18 19 20 21 22

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 16 18

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 12 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31324
Entity Detected:	2010107G23Rik, RIKEN cDNA 2010107G23 gene ( MGI:1917144)
Sequence:	sense strand is shown >T31324 TTAGGGGCTGCAAAGCTGGGGTCCCCCAGAGCCGCAGCTGCAGCTCACACACCTTGTGGTGGGAGGTTGG ACTTCGTCCTTTACCTGGGTGTTCCCTGCAGGGTTTCACTTAGCAGAAGCTGGCCTTTAACCCCTGACCT TCCAGCCTGTACCTCCTTGGAGTTCTGGTGTTGATTACAGGTACTGCACAGCAGAAATTCCCAGCGTCCA GGGAGAATGGTGAGGATCTTGGCCAATGGGGAGATCGTTCAGGATGATGACCCACGAGTGAGGACGACCA CCCAGCACAGAAGTAGTAGCTCTCAGCAGGGCTTTTTCAACAGAGGCCACGGCGCACCTCCAGGGGGCCC TGGACCCCGCCAGCAGCAGGCAGGTGCCCGACTGGGTGCTGCCCAATCTCCTTTCAGTGACCTGAACCGG CAGCTGGTGAACATGGGCTTCCCACAATGGCACCTTGGGAACCACGTGGTGGAACCTGTGACCTCCATCC TCCTGCTCTTCCTGCTTATGATGCTCGGGGTTCGTGGCCTCCTGCTTGTGGGCCTGGTCTACCTGGTGTC TCACCTGAGTCAGCGGTGACCTCCGGGGGACTCATGAGGGCGGGAGTGTTCAGTAGGATCGACTGGACTG GGCTTTGGTGCGACATCATGCACAGGGGGAGGGAGCTTGGGTGGGCTCAGAAGGTGTGATGGGCATGAGA CCGGCTTTGTAGATTTTTCTCCCGCTGCAGGAGGCAGAGGGACTCTCTGGGCCAGCATTGCTGAAAAAGA GAGTCCTGTGGTTTCCTGGAATGTTTTTAGGGGGCATAGGGCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3989515), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22036. Forward Primer - name:022036_F_IRAV51-54_H18_2010107G23Rik, sequence:TTAGGGGCTGCAAAGCTG; Reverse Primer - name:022036_R_SP6_IRAV51-54_H18_2010107G23Rik, sequence:GAAGCCCTATGCCCCCTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20188 same embryo
	EMAGE:20190 same embryo
	EMAGE:20186 same embryo
	EMAGE:20189 same embryo
	EurExpress:euxassay_012442 same experiment
	MGI:4822646 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source