EMAGE mouse gene expression spatial database

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EMAGE:20298

Ntm neurotrimin ( MGI:2446259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012548. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012548_01	euxassay_012548_02	euxassay_012548_03	euxassay_012548_04

euxassay_012548_05	euxassay_012548_06	euxassay_012548_07	euxassay_012548_08	euxassay_012548_09

euxassay_012548_10	euxassay_012548_11	euxassay_012548_12	euxassay_012548_13	euxassay_012548_14

euxassay_012548_15	euxassay_012548_16	euxassay_012548_17	euxassay_012548_18	euxassay_012548_19

euxassay_012548_20	euxassay_012548_21	euxassay_012548_22	euxassay_012548_23	euxassay_012548_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20298_wholemount_strong.wlz

20298_wholemount_moderate.wlz

20298_wholemount_weak.wlz

20298_wholemount_possible.wlz

20298_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20298_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 01 03

forelimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 20 21

forelimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 19 20 21 23

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 17 18

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 01 15 16 17 18

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 01 03 15 16 17 18

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 15 16 17 18

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 16 17

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

hindbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 05 06 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 09 18 19 20 21

spinal cord mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

spinal cord meninges

strong

regional

strong expression: see section 12 13 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 13 14 15 16 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 23 24

axial skeleton tail region

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31438
Entity Detected:	Ntm, neurotrimin ( MGI:2446259)
Sequence:	sense strand is shown >T31438 ACCTCCAGGGTCCACCTCATTGTACAAGTATCTCCCAAAATTGTAGAGATTTCTTCAGATATCTCCATTA ATGAAGGGAACAACATCAGCCTCACTTGCATAGCCACAGGTAGACCGGAGCCTACAGTAACCTGGAGACA TATTTCTCCCAAGGCCGTTGGCTTTGTGAGTGAGGATGAGTACCTGGAGATCCAGGGCATCACTCGGGAA CAGTCAGGCGAGTACGAGTGCAGCGCCTCCAACGACGTGGCGGCACCAGTGGTACGAAGAGTGAAGGTCA CCGTGAACTATCCACCATACATCTCAGAAGCTAAGGGCACAGGTGTCCCCGTGGGGCAGAAGGGGACTCT GCAGTGTGAAGCTTCCGCAGTCCCTTCAGCAGAATTTCAATGGTTCAAGGATGACAAAAGACTGGTCGAA GGAAAGAAGGGAGTCAAAGTGGAAAACAGACCTTTCCTTTCAAAACTCACCTTTTTCAACGTCTCTGAAC ATGACTATGGGAACTACACATGTGTGGCCTCCAACAAGCTGGGTCACACCAACGCCAGCATCATGCTATT TGGTCCCGGTGCTGTCAGTGAGGTCAACAATGGGACATCAAGGAGGGCAGGCTGCATTTGGCTCCTCCCT CTTCTGGTCTTACACCTGCTCCTCAAATTTTGATGTGAGTGCCCCTTCCCTGCTGGGGAGAGCTGCTGCC ACCGCATCTCAATACAACAGCACTGCAAAATGAAGCAACAAGTCAGAATCAAATGAAATTCCGAGAATCA CAGCCAATGAGACAGAAATTCGAGGGAGGGGGACAAAGCATACTGTGGTAAAGGGGAAAAAAAGGTTTAA GAAAAGGAAATTTGGAAATTGCCTTGCAGATATTTCGGTACCGCTGAGTTTTCTTTCTTTTCCCAAGTGG GAAGAAGGCACACCTAGCTTGGACCCACCCACAAGCTGCACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4480983), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 25631. Forward Primer - name:025631_F_IRAV59-62_G06_Hnt, sequence:ACCTCCAGGGTCCACCTC; Reverse Primer - name:025631_R_SP6_IRAV59-62_G06_Hnt, sequence:CAGTGCAGCTTGTGGGTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20295 same embryo
	EMAGE:20296 same embryo
	EMAGE:20297 same embryo
	EMAGE:20294 same embryo
	EMAGE:20293 same embryo
	EurExpress:euxassay_012548 same experiment
	MGI:4826816 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source