EMAGE mouse gene expression spatial database

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EMAGE:20416

Tpr translocated promoter region ( MGI:1922066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012642. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012642_01	euxassay_012642_02	euxassay_012642_03	euxassay_012642_04

euxassay_012642_05	euxassay_012642_06	euxassay_012642_07	euxassay_012642_08	euxassay_012642_09

euxassay_012642_10	euxassay_012642_11	euxassay_012642_12	euxassay_012642_13	euxassay_012642_14

euxassay_012642_15	euxassay_012642_16	euxassay_012642_17	euxassay_012642_18	euxassay_012642_19

euxassay_012642_20	euxassay_012642_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20416_wholemount_strong.wlz

20416_wholemount_moderate.wlz

20416_wholemount_weak.wlz

20416_wholemount_possible.wlz

20416_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20416_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

vibrissa

weak

regional

weak expression: see section 05 19 20

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 12 weak expression: see section 11 14 15 16

lower jaw molar

weak

regional

weak expression: see section 07 08 19

upper jaw incisor

weak

regional

weak expression: see section 11 14 15 16

upper jaw molar

weak

regional

weak expression: see section 06 07 08 19

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

renal cortex

weak

regional

weak expression: see section 06 07 08 09 15 16 17 18

testis

weak

regional

weak expression: see section 04 05 06 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31602
Entity Detected:	Tpr, translocated promoter region ( MGI:1922066)
Sequence:	sense strand is shown >T31602 TGAGCAGAGGGATGAGCCTCAAGAACCCACTAATAAGGCTCCAGAACAGCAAAGACAAATCACACTGAAA ACCACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGCACATCAGACCCACCAACAGCCAATATCAAGCCAA CTCCTGTTGTTTCTACTCCAAGTAAAGTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGC AAGTATCCGCCCAATGGTTACACCTGCAACGGTCACAAATCCGACCACTACCCCAACAGCAACCGTAATG CCTACTACACAGGTGGAGTCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTATTTG GAAACGCAAGTGGATCTGTTCGTTCTACTAGTCCTAATGTTCAGCCCTCCATTTCTCAGCCCATCCTAAC TGTACAGCAGCAAACACAAGCTACAGCTTTTGTACAACCCACTCAACAGAGCCACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3582857), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61859. Forward Primer - name:061859_F_IRAV77-80_G06_Tpr, sequence:TGAGCAGAGGGATGAGCC; Reverse Primer - name:061859_R_SP6_IRAV77-80_G06_Tpr, sequence:CGGGTGGCTCTGTTGAGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20419 same embryo
	EMAGE:20420 same embryo
	EMAGE:20417 same embryo
	EMAGE:20415 same embryo
	EMAGE:20418 same embryo
	EurExpress:euxassay_012642 same experiment
	MGI:4828883 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source