EMAGE mouse gene expression spatial database

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EMAGE:20478

Diap3 diaphanous homolog 3 (Drosophila) ( MGI:1927222)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012699. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012699_01	euxassay_012699_02	euxassay_012699_03	euxassay_012699_04

euxassay_012699_05	euxassay_012699_06	euxassay_012699_07	euxassay_012699_08	euxassay_012699_09

euxassay_012699_10	euxassay_012699_11	euxassay_012699_12	euxassay_012699_13	euxassay_012699_14

euxassay_012699_15	euxassay_012699_16	euxassay_012699_17	euxassay_012699_18	euxassay_012699_19

euxassay_012699_20	euxassay_012699_21	euxassay_012699_22	euxassay_012699_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20478_wholemount_strong.wlz

20478_wholemount_moderate.wlz

20478_wholemount_weak.wlz

20478_wholemount_possible.wlz

20478_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20478_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 17 18 19

pituitary gland

weak

regional

weak expression: see section 11 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 04 05 19 20

hypothalamus ventricular layer

weak

regional

weak expression: see section 14

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 12 13 14

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 14 15 weak expression: see section 08 09

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 13 14 15 18 19 weak expression: see section 02 03 04 05 06 07 08 09 16 17 20 21 22 23

midbrain ventricular layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17

lower jaw incisor

weak

regional

weak expression: see section 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 07 weak expression: see section 18 19

upper jaw incisor

weak

regional

weak expression: see section 10 11 14 15

upper jaw molar

moderate

regional

moderate expression: see section 07 weak expression: see section 06 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38209
Entity Detected:	Diap3, diaphanous homolog 3 (Drosophila) ( MGI:1927222)
Sequence:	sense strand is shown >T38209 TTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAA ACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCAT TGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGT GAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATC AAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGC TGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGA CCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTG GTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAG CTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 147413. Forward Primer - name:147413_F_cDNA_Diap3, sequence:TTCAATCTTCCTGAGCTCCTTC; Reverse Primer - name:147413_N_SP6_cDNA_Diap3, sequence:CTTCCCATCTGCTTCACGTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20481 same embryo
	EMAGE:20479 same embryo
	EMAGE:20477 same embryo
	EMAGE:20480 same embryo
	EMAGE:20476 same embryo
	EurExpress:euxassay_012699 same experiment
	MGI:4824305 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source