EMAGE mouse gene expression spatial database

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EMAGE:20505

Rnaset2b ribonuclease T2B ( MGI:3702087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012749. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012749_01	euxassay_012749_02	euxassay_012749_03	euxassay_012749_04

euxassay_012749_05	euxassay_012749_06	euxassay_012749_07	euxassay_012749_08	euxassay_012749_09

euxassay_012749_10	euxassay_012749_11	euxassay_012749_12	euxassay_012749_13	euxassay_012749_14

euxassay_012749_15	euxassay_012749_16	euxassay_012749_17	euxassay_012749_18	euxassay_012749_19

euxassay_012749_20	euxassay_012749_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20505_wholemount_strong.wlz

20505_wholemount_moderate.wlz

20505_wholemount_weak.wlz

20505_wholemount_possible.wlz

20505_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20505_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 07 08 16 moderate expression: see section 09 14 15

thymus primordium

weak

regional

weak expression: see section 09 10 11 12

pancreas

moderate

regional

moderate expression: see section 08 09 10

thyroid gland

moderate

regional

moderate expression: see section 12 13

vibrissa

moderate

regional

moderate expression: see section 20 weak expression: see section 19

diencephalon roof plate

moderate

regional

moderate expression: see section 11

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata floor plate

moderate

regional

moderate expression: see section 11 weak expression: see section 10

metencephalon floor plate

moderate

regional

moderate expression: see section 11 weak expression: see section 10

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain roof plate

weak

regional

weak expression: see section 10

mandible

weak

regional

weak expression: see section 04 05 06 07 08 10 15 16 17 18 19

metanephros

weak

regional

weak expression: see section 07 08 09 16 17

ovary

moderate

regional

moderate expression: see section 05 06 17 18

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38231
Entity Detected:	Rnaset2b, ribonuclease T2B ( MGI:3702087)
Sequence:	sense strand is shown >T38231 AGGTTAACAGCTGCCAAGACTCTCTGGATTACTGGACAATACATGGACTATGGCCCGATAGAGCAGAAGA TTGTAACCAGTCCTGGCACTTTAACTTAGATGAGATTAAGGACCTTTTGCGAGACATGAAGATCTACTGG CCCGATGTGATTCACCGGTCTTCTAATCGCAGCCAATTCTGGAAACATGAGTGGGTTAAACACGGCACCT GTGCTGCCCAGGTAGACGCCCTCAATTCCGAGAAGAAGTACTTTGGGAAGAGCCTGGATCTGTACAAGCA GATTGACCTCAACAGTGTGCTACAAAAATTTGGGATCAAGCCATCCATCAACTACTACCAGCTTGCAGAT TTCAAAGATGCACTTACCAGAATCTATGGTGTGGTGCCTAAAATCCAGTGCCTTATGCCAGAACAGGGAG AGAGCGTGCAGACCGTTGGCCAGATAGAGCTGTGCTTCACCAAGGAGGACTTACATTTGCGGAACTGCAC TGAGCCAGGGGAGCAGCTGTCCTCCAGGCAGGAAGCCTGGCTGGCCATGGAGGCTTCTACACATGGGATG ATGGTCTGTGAAGACGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141955. Forward Primer - name:141955_F_cDNA_Rnaset2, sequence:AGGTTAACAGCTGCCAAGACTC; Reverse Primer - name:141955_N_SP6_cDNA_Rnaset2, sequence:CCGTCTTCACAGACCATCATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20506 same assay
	EMAGE:20508 same embryo
	EMAGE:20507 same embryo
	EMAGE:20503 same embryo
	EMAGE:20504 same embryo
	EurExpress:euxassay_012749 same experiment
	MGI:4827758 same experiment
	MGI:4827757 same experiment
	EMAGE:20509 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source