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EMAGE:20576

Sec14l1 SEC14-like 1 (S. cerevisiae) ( MGI:1921386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576
"Pseudo-wholemount" of euxassay_013817. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013817_01 euxassay_013817_02 euxassay_013817_03 euxassay_013817_04
EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576
euxassay_013817_05 euxassay_013817_06 euxassay_013817_07 euxassay_013817_08 euxassay_013817_09
EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576
euxassay_013817_10 euxassay_013817_11 euxassay_013817_12 euxassay_013817_13 euxassay_013817_14
EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576
euxassay_013817_15 euxassay_013817_16 euxassay_013817_17 euxassay_013817_18 euxassay_013817_19
EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576 EMAGE:20576
euxassay_013817_20 euxassay_013817_21 euxassay_013817_22 euxassay_013817_23 euxassay_013817_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20576Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20576_wholemount_strong.wlz
20576_wholemount_moderate.wlz
20576_wholemount_weak.wlz
20576_wholemount_possible.wlz
20576_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20576_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex marginal layer
weak weak
regionalweak expression: see section 03 04 08 09 10 11 12 14 15 16 17 18 19 20
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38212
Entity Detected:Sec14l1, SEC14-like 1 (S. cerevisiae) ( MGI:1921386)
Sequence:sense strand is shown

>T38212
CAAAGGGGACATCGTCTTTAACATTTATCACTCCAAGAGGTCTCCCCAGCCACCCAAGAAGGACTCGCTA
GGGGCTCACAGCATCACTTCCCCAGGAGGCAACAACGTGCAGCTGATAGACAAAGTCTGGCAGCTGGGCC
GGGACTATAGCATGGTGGAGTCTCCTCTAATCTGCAAAGAAGGAGAGAGCGTGCAGGGGTCCCATGTGAC
CAGGTGGCCAGGCTTCTACATCCTACAGTGGAAATTCCACACCATGCCAGCATGTGCTGCCACCAACCTA
CCCCGAGTGGACGACGTGCTGGCCTCCCTGCAGGTCTCCTCCCACAAGTGCAAAGTCATGTACTACACAG
AAGTGATCGGCTCTGAAGATTTCAGAGGCTCCATGACCAGCCTGGAATCTAGCCACAGCGGGTTCTCACA
GCTCAGTGCCGCCACCACCTCCTCCAGCCAGTCGCAATCCAGCTCCATGATTTCCAGATGGCGATTTTGC
TGACATCCTCCAAGAAAGCGCTTCACTCGACAGCCCACATGTGCCCAGAGATGTTTGTAGACCTATCTGA
GTCGCAGCCATGTCCCAGGCTGAAGAGCTGTTCCCCTTAAGTCCATGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142150. Forward Primer - name:142150_F_cDNA_Sec14l1, sequence:CAAAGGGGACATCGTCTTTAAC; Reverse Primer - name:142150_N_SP6_cDNA_Sec14l1, sequence:GCATGGACTTAAGGGGAACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20573 same embryo
 EMAGE:20572 same embryo
 EMAGE:20574 same embryo
 EMAGE:20575 same embryo
 EMAGE:20571 same embryo
 EurExpress:euxassay_013817 same experiment
 MGI:4827932 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS