EMAGE mouse gene expression spatial database

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EMAGE:20679

Musk muscle, skeletal, receptor tyrosine kinase ( MGI:103581)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013976. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013976_01	euxassay_013976_02	euxassay_013976_03	euxassay_013976_04

euxassay_013976_05	euxassay_013976_06	euxassay_013976_07	euxassay_013976_08	euxassay_013976_09

euxassay_013976_10	euxassay_013976_11	euxassay_013976_12	euxassay_013976_13	euxassay_013976_14

euxassay_013976_15	euxassay_013976_16	euxassay_013976_17	euxassay_013976_18	euxassay_013976_19

euxassay_013976_20	euxassay_013976_21	euxassay_013976_22	euxassay_013976_23	euxassay_013976_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20679_wholemount_strong.wlz

20679_wholemount_moderate.wlz

20679_wholemount_weak.wlz

20679_wholemount_possible.wlz

20679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19 20 21

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

hand

strong

regional

strong expression: see section 02 03 04 05 24

foot

strong

regional

strong expression: see section 06 07 08 21 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 02 03 04 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 19 20 21 22 23 24

heart ventricle

moderate

spotted

moderate expression: see section 13 14 17 18

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

tail paraxial mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39430
Entity Detected:	Musk, muscle, skeletal, receptor tyrosine kinase ( MGI:103581)
Sequence:	sense strand is shown >T39430 TCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTCCAGCTCTTCATCACAAAGCCAGGACTCTAC ACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTACCGCAAAGGCTGCAGCCACTGTCAGCATAG CAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGTTACTGTGCCCAGTACAGAGGGGAGGGTGTTTTGAT GCAAGGCCCTGGCGAAAAGATGCTCTTGGTTTTTCTTCCAACAACCTCCCACCGGGACCCCGAGGACGCC CAGGAGCTGCTGATCCACACTGCGTGGAATGAACTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTG AGGCTCTGCTGTGTTACCACCTCTTCCTAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTG CAGAGAGTACTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCC CACCGGGGCCTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGAAAGCTTCCCAGCATGC ACCGGGACCCCACAGCCTGCACAAGACTGCCATATTTAGATTATAAAAAGGAAAACATAACAACATTCCC GTCAATAACGTCCTCCAGGCCGAGCGCGGACATTCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTC TCGCCTGCGTACTCCATGACCGTCATCATCTCCATCGTGTCCAGCTTGGCCCTGTTTGCTCTTCTCACCA TCGTTACTCTCTATTGCTGCCGAAGGAGGAAAGAATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGAC CCTCACCACGTTGCCTTCCGAGCTCCTGCTGGATAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCA CTCCTTCTGAATCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99274. Forward Primer - name:099274_F_cDNA_Musk, sequence:TCAAGAGAGTGTGAAAGACCGA; Reverse Primer - name:099274_N_SP6_cDNA_Musk, sequence:GGATTCAGAAGGAGTGGCATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20677 same embryo
	EMAGE:20676 same embryo
	EMAGE:20678 same embryo
	EMAGE:20675 same embryo
	EMAGE:20680 same embryo
	EurExpress:euxassay_013976 same experiment
	MGI:4826514 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source