EMAGE mouse gene expression spatial database

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EMAGE:6880

Reep3 receptor accessory protein 3 ( MGI:88930)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004457. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004457_01	euxassay_004457_02	euxassay_004457_03	euxassay_004457_04

euxassay_004457_05	euxassay_004457_06	euxassay_004457_07	euxassay_004457_08	euxassay_004457_09

euxassay_004457_10	euxassay_004457_11	euxassay_004457_12	euxassay_004457_13	euxassay_004457_14

euxassay_004457_15	euxassay_004457_16	euxassay_004457_17	euxassay_004457_18	euxassay_004457_19

euxassay_004457_20	euxassay_004457_21	euxassay_004457_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:6880

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6880_wholemount_strong.wlz

6880_wholemount_moderate.wlz

6880_wholemount_weak.wlz

6880_wholemount_possible.wlz

6880_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6880_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 01 02 03 20 21 22

foot

moderate

regional

moderate expression: see section 17 19

hindlimb digit 1

moderate

regional

moderate expression: see section 18

hindlimb digit 2

moderate

regional

moderate expression: see section 18

hindlimb digit 3

moderate

regional

moderate expression: see section 18

hindlimb digit 4

moderate

regional

moderate expression: see section 18

hindlimb digit 5

moderate

regional

moderate expression: see section 18

lower leg

moderate

regional

moderate expression: see section 20 21 22

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

spinal cord ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

heart valve

moderate

regional

moderate expression: see section 10 11 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6437
Entity Detected:	Reep3, receptor accessory protein 3 ( MGI:88930)
Sequence:	sense strand is shown >T6437 GAGGGCGCCAGCGCGGCCCCGGGGAGCGCGAGCGGGGAGCGCGCGGCCTGCGGTCAGCGGCCCATCGGCC GAAAAGATGGTGTCCTGGATGATCTCCCGAGCCGTGGTGCTGGTGTTTGGAATGCTCTATCCAGCGTACT ATTCCTACAAAGCCGTGAAGACGAAAAACGTCAAGGAATACGTTCGCTGGATGATGTATTGGATCGTCTT TGCCCTCTACACTGTCATTGAAACGGTGGCCGATCAGACACTTGCATGGTTTCCCCTGTACTATGAGCTG AAGATTGCCTTCGTCATTTGGCTGCTGTCGCCCTACACTAGAGGGGCGAGTTTAATCTATAGAAAGTTCC TTCATCCCCTGCTGTCATCAAAGGAAAGGGAAATTGATGATTATATTGTCCAAGCCAAAGAAAGAGGCTA TGAGACAATGGTGAATTTTGGACGGCAAGGTTTGAATTTAGCAGCTGCAGCCGCCGTCACTGCAGCAGTG AAGAGCCAAGGAGCAATAACGGAGCGTCTGCGAAGTTTCAGCATGCATGATCTGACAGCTATCCAAGGGG ATGAGCCCGTGGGACACAGACCCTACCAGACTTTGCCAGAAGCAAAGAGGAAAGGCAAACAAGCCACCGA GTCACCAGCCTATGGAATTCCACTGAAAGATGGAAGTGAGCAGACAGACGAAGAAGCGGAGGGGCCATTC TCCGATGACGAGATGGTGACTCACAAGGCGCTGAGGCGATCCCAGAGCATGAAATCTGTCAAGACCATCA AAGGCCGCAAAGAGGTGCGGTATGGCTCACTAAAATATAAAGTGAAGAAGAGACCGCAAGTGTATTTTTA GTGTCTACACCTTGCCCTTCCCAAGATGAATAAACCGCTAACAGATCAACTTCATTTTCTAACCTGATGT ATTCAGGGCTTAACACTTTAAATCTTCAGAGTGTGGCAGGGATGGGATTTACGCAATGACTTTAAATGGT CTGTGTTTCTTTAACAATTACTTTTAGATGTTTAACTGCTAAACTTAGTTATGTAAGGTTTGTAAATGTA CGTGTAGATAACAAAAGTGACCGAGAATCGCGTCCCTGATGTCAGCAGCAACACTGAGTGCCTAGGGCCT AAGGATAAAGCCTAGCTAGACCCAGGGCAGACTTCAAAAGTCTCTGCTCCAGGACACACCTGCCTGCCGT TCTGGTGTTGCATTCTGTTTACATCATCTTGCCTGCATCTATCACAGGAGAGTGGTGACAGCCCCTGAGG AGCCAGTGTCATTGCCATAGGAGGGGGCTGTGTCCTTCCTTAAGCCTTAGACGCTGAAAGTTTTTTAAAC AAATGATTTGAAAACCAGGTGTCCACATAGCACTTTCCTGGCTAGTTTTGCACACTTGAAAATTGTTTAC TTATTTTACAACTGTACATTTACATTTTCCTGATGCTGTCTGTTTTGTTGTCTGTCATATTCCGACATGT CAAGATCATGCCTTGAAACCGCGTCATCTCCTTTTCCTATGGGTTTGTTTCGTTTGGTAAATCCATTCAG CCCATTATAGACACATAACTGTTAAGTTTGTAGTCTTTTGTTTTGTTTTATTGAGGTAGGGTCTCTTCCC TGATCCTCGTCCTGCTTCCACTTCGCCATTGCTGGGATAACAGACGTTATCACACTTGGCTCATTTTAAA TGCCCTTCAGTTATATTCCGGACATCTGAGAAGTTATCTTAAACATTAACCAGTTTCCATCATTGAATTT GATGTGGTTTTTCTCAACAGCAAATTATTCTAGGTTATTCTAGGTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3585133 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3585133 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6882 same embryo
	EMAGE:6879 same embryo
	EMAGE:6881 same embryo
	EMAGE:6878 same embryo
	EurExpress:euxassay_004457 same experiment
	MGI:4827682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source