EMAGE mouse gene expression spatial database

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EMAGE:7000

Plxdc2 plexin domain containing 2 ( MGI:1914698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002573. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002573_01	euxassay_002573_02	euxassay_002573_03	euxassay_002573_04

euxassay_002573_05	euxassay_002573_06	euxassay_002573_07	euxassay_002573_08	euxassay_002573_09

euxassay_002573_10	euxassay_002573_11	euxassay_002573_12	euxassay_002573_13	euxassay_002573_14

euxassay_002573_15	euxassay_002573_16	euxassay_002573_17	euxassay_002573_18	euxassay_002573_19

euxassay_002573_20	euxassay_002573_21	euxassay_002573_22	euxassay_002573_23	euxassay_002573_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7000_wholemount_strong.wlz

7000_wholemount_moderate.wlz

7000_wholemount_weak.wlz

7000_wholemount_possible.wlz

7000_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7000_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 03 04 17

humerus

moderate

regional

moderate expression: see section 02 03 04 05 19 20 21 22

body-wall mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19 20

epidermis

strong

regional

strong expression: see section 19 20 21 moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 22 23 24

vibrissa

strong

regional

strong expression: see section 19 20 moderate expression: see section 06 07

hypothalamus mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 moderate expression: see section 09

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 19 20

pons mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 11

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 10 11

ventral grey horn

moderate

single cell

moderate expression: see section 09 10 11 12 13 14

cochlear duct

strong

regional

strong expression: see section 07 08 16 17 18 19 20

tongue epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5182
Entity Detected:	Plxdc2, plexin domain containing 2 ( MGI:1914698)
Sequence:	sense strand is shown >T5182 GCCCCATCCCCAGGGATAACCCCGGAGCCCAGAGCCTCAAGAAAAAATTCGTTGGGCAGGGGAGAGAGGT CGCGGCAGCGGCATGGCAAGGTTCTGGAGGGCCGACCTGGCCGCAGCAGGAGTTATGTTACTTTGTCACT TTTTAACAGACCGGTTCCAGTTCGCCCACGGGGAGCCTGGACACCATACCAATGATTGGATTTATGAAGT TACAAACGCTTTTCCTTGGAATGAAGAGGGGGTAGAAGTGGACTCTCAAGCATACAACCACAGGTGGAAA AGAAATGTGGACCCTTTTAAGGCAGTAGACACAAACAGAGCCAGCATGGGCCAAGCCTCTCCAGAGTCCA AAGGGTTCACTGACCTGCTACTGGATGACGGACAGGACAATAACACCCAGATAGAGGAGGACACGGATCA CAATTACTACATTTCTCGGATATATGGTCCAGCGGATTCTGCCAGCCGGGATCTGTGGGTTAACATAGAC CAAATGGAAAAAGACAAAGTGAAGATTCACGGGATACTTTCCAACACTCATCGGCAAGCTGCAAGAGTGA ATCTGTCCTTCGATTTTCCATTTTATGGTCATTTTCTAAATGAAGTCACTGTGGCAACTGGGGGTTTCAT ATATACTGGAGAAGTTGTACATCGAATGCTCACAGCTACACAGTATATAGCTCCTTTAATGGCAAATTTT GATCCCAGTGTATCCAGAAATTCAACTGTCAGATATTTTGATAATGGCACAGCTCTTGTTGTCCAGTGGG ACCATGTCCACCTGCAGGATAATTACAACCTGGGAAGCTTCACATTCCAGGCCACACTCCTCATGGACGG GCGCATCATCTTTGGATACAAAGAAATCCCTGTCTTGGTCACACAGATAAGTTCTACCAACCATCCAGTG AAAGTCGGGTTGTCTGATGCATTTGTCGTGGTCCACAGGATCCAGCAAATACCCAATGTTCGAAGAAGAA CAATTTATGAATATCACCGAGTAGAACTACAAATGTCCAAAATTACCAACATCTCAGCTGTGGAGATGAC TCCACTTCCCACATGTCTCCAGTTCAATGGTTGTGGCCCTTGTGTGTCCTCGCAGATTGGTTTCAACTGC AGTTGGTGCAGCAAACTTCAAAGATGCTCCAGTGGATTTGATCGCCATCGGCAGGACTGGGTGGACAGTG GATGCCCGGAAGAGGTACAGTCAAAAGAGAAGATGTGTGAGAAGACAGAGCCAGGAGAGACATCTCAAAC TACCACGACCTCCCACACGACCACCATGCAATTCAGGGTCCTGACCACCACCAGGAGAGCTGTGACATCT CAGATGCCTACCAGCCTGCCTACAGAAGATGACACGAAGATAGCCCTACATCTCAAAGACAGTGGAGCCT CCACAGATGACAGTGCAGCTGAGAAGAAAGGAGGAACCCTCCATGCAGGCCTCATTGTTGGAATTCTCAT CTTGGTCCTCATTATAGCAGCGGCCATTCTGGTGACAGTGTATATGTATCACCATCCAACATCAGCAGCC AGCATCTTCTTCATTGAGAGACGCCCAAGCAGATGGCCAGCAATGAAGTTTCGAAGAGGCTCAGGACACC CTGCCTATGCAGAAGTTGAACCAGTTGGAGAGAAAGAAGGTTTTATTGTATCAGAGCAGTGCTAAAATTT TAGGACAGAGCAGCACCAGTACTGGCTTACAGGTGTTAAGACTAAAACTTTGCTTATGCATTTAAGACAA ACAGACACACAACCCACAACCACACACAAAGGAGCCCTAAACTGCTGTAGACAGAAGGGCGACGAGATTT CTGGACAAGCCCAGCCCAGGAACATTGAAAGGAAAACTCAGACTTGTACAAGACACCATGTACAATGATT AAAGAATTCCCTAGTGGAATGACATCCATGGTTCACAAGGAACATCTCCGGTGGACTTGCCAGGAGTGTG ACGAGATGACGATGCTTTTGGTTTAGGTGCAGGGTTGCAAAGAAATCAAGGAAAAAAAATATGACAATAA ATAAAGCTTTAGTTCACAAGGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5349869 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5349869 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7003 same embryo
	EMAGE:7002 same embryo
	EMAGE:7004 same embryo
	EMAGE:7001 same embryo
	EMAGE:7005 same embryo
	EurExpress:euxassay_002573 same experiment
	MGI:4827289 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source