EMAGE mouse gene expression spatial database

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EMAGE:7189

Ccnd1 cyclin D1 ( MGI:88313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002703. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002703_01	euxassay_002703_02	euxassay_002703_03	euxassay_002703_04

euxassay_002703_05	euxassay_002703_06	euxassay_002703_07	euxassay_002703_08	euxassay_002703_09

euxassay_002703_10	euxassay_002703_11	euxassay_002703_12	euxassay_002703_13	euxassay_002703_14

euxassay_002703_15	euxassay_002703_16	euxassay_002703_17	euxassay_002703_18	euxassay_002703_19

euxassay_002703_20	euxassay_002703_21	euxassay_002703_22	euxassay_002703_23	euxassay_002703_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7189_wholemount_strong.wlz

7189_wholemount_moderate.wlz

7189_wholemount_weak.wlz

7189_wholemount_possible.wlz

7189_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7189_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09

vibrissa

strong

regional

strong expression: see section 03 04 05 21 22

olfactory cortex ventricular layer

weak

regional

weak expression: see section 10 11 16 17

telencephalon ventricular layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 10 16 17

cervical ganglion

strong

regional

strong expression: see section 08 09 17

thoracic ganglion

strong

regional

strong expression: see section 11 12 13 14

neural retina

strong

regional

strong expression: see section 01 02 03 04 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

kidney calyx

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19 20

orbito-sphenoid

strong

regional

strong expression: see section 21 moderate expression: see section 02 03 04 05 06 07 17 18 19 20 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5858
Entity Detected:	Ccnd1, cyclin D1 ( MGI:88313)
Sequence:	sense strand is shown >T5858 GGGCTGTCGGCGCAGTAGCAGAGAGCTACAGACTTCGCGCGCTCCGGAGACCGGCAGTACAGCGCGAGGC AGCGCGCGTCAGCAGCAGCCACCGGAGCCCAACCGAGACCACAGCCCTCCCCAGACGGCCGCGCCATGGA ACACCAGCTCCTGTGCTGCGAAGTGGAGACCATCCGCCGCGCGTACCCTGACACCAATCTCCTCAACGAC CGGGTGCTGCGAGCCATGCTCAAGACGGAGGAGACCTGTGCGCCCTCCGTATCTTACTTCAAGTGCGTGC AGAAGGAGATTGTGCCATCCATGCGGAAAATCGTGGCCACCTGGATGCTGGAGGTCTGTGAGGAGCAGAA GTGCGAAGAGGAGGTCTTCCCGCTGGCCATGAACTACCTGGACCGCTTCCTGTCCCTGGAGCCCCTGAAG AAGAGCCGCCTGCAGCTGCTGGGGGCCACCTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCATTCCCT TGACTGCCGAGAAGTTGTGCATCTACACTGACAACTCTATCCGGCCCGAGGAGCTGCTGCAAATGGAACT GCTTCTGGTGAACAAGCTCAAGTGGAACCTGGCCGCCATGACTCCCCACGATTTCATCGAACACTTCCTC TCCAAAATGCCAGAGGCGGATGAGAACAAGCAGACCATCCGCAAGCATGCACAGACCTTTGTGGCCCTCT GTGCCACAGATGTGAAGTTCATTTCCAACCCACCCTCCATGGTAGCTGCTGGGAGCGTGGTGGCTGCGAT GCAAGGCCTGAACCTGGGCAGCCCCAACAACTTCCTCTCCTGCTACCGCACAACGCACTTTCTTTCCAGA GTCATCAAGTGTGACCCGGACTGCCTCCGTGCCTGCCAGGAACAGATTGAAGCCCTTCTGGAGTCAAGCC TGCGCCAGGCCCAGCAGAACGTCGACCCCAAGGCCACTGAGGAGGAGGGGGAAGTGGAGGAAGAGGCTGG TCTGGCCTGCACGCCCACCGACGTGCGAGATGTGGACATCTGAGGGCCACCGGGCAGGCGGGAGCCACCA AGTAGTGGCACCCGCAAAGAGGAAGGAGCCAGCCCGGGTGCTCCTGACGACGTCCCCCTTGGGGACATGT TGTTACCAGAAGAGGAAGTTTTGTTCTCTTTGTTGGTTGTTTTTCCTTAATCTTTCTCCTTTCTATCTGA TTTAAGCAAAAGAGAAAAAAATATCTGAAAGCTGTCTTAAAGAGAGAGAGAGAGAGAGATAGAATCTGCA TCACCCTGAGAGTAGGGAGCCAGGGGGTGCTACAAAAATAGAATTCTGTACCCCAGTAATCAACTAGTTT TCTATTAATGTGCTTGTCTGTTCTAAGAGTAGGATTAACACAGGGGAAGTCTTGAGAAGGAGTTTTGATT CTTTTATATGTTTTAAAAAAAAAAGCTTAAGAAACATTGCTTTAAAAAGGAAGGAAAAAAAATACAGCAA ACCATTGTTAAAGTAGAAGAGTTTTTAGGTTGAGAAATGTACTCTGCTTTGCTGAAAAGCCACAGCTTAG GCCCTCAGCCTCACTCCCTGGCTTGCTCAGTGCCTACAGCCCTGTTACCTGATACCTGTGCTTTATCCCA GGGGTGGGCAGACCTCTTAACCTTATAGATGGTCAGTGCGACCTCTAGTGGTCTCATGGCGTGTGGCACA ACCCCCCTCCCCAGGGCTCAGCTTAATGTGCCCTCTCCCCCCAACAACCTGCAGGTTCACAGCGCCAGCC ACACAGCGGTAGGGATGAAATAGTGACATAATATATTCTATTTTTGTAACCTTCCTATTTTGTAGCTCTG TTTAGAGAGATGCTGGTTTTTGCCTGAAGGCCCTGCAGCCTGCCCACATCAGGTTAAACCCACAGCTTTT GTGTGTGGTTTGTTTTGTTGTGTTTTCTTTCTCTATGTTCCAAAACCATTCCATTTCAAAGCACTTTTGG TCAGCTAGCTGGAGGCAGTGTTGCTGGTGTGTGTTGGGGGGAGGGGTTCTAATGGAATGGATGGGGATGT CCACACACGCATTCAGATGGCTGTACAACAGGTTGTAGGGCTGGTAGTATGAGGTGCTTGGGAAGTTTTG TTGGGTCAAGAAGAGAGAACTCTGTTCTCGCACCACCGGGATCTGTCCTGCAAAGTTGAAGGGATCCTTT GGTGCCAGCTGGTGTTTGGAAGTAGGAACCATGATGGCATTACCTGGACAAGGAGATTGGGGACAACTCT TAAGTCTCACACAGGAGGCTTTTAAACACTAAAATATTTCGAAAACTGATCTGCTTCAATAATTTAATTT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3155470 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155470 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7193 same embryo
	EMAGE:7191 same embryo
	EMAGE:7192 same embryo
	EMAGE:7190 same embryo
	EMAGE:7188 same embryo
	EurExpress:euxassay_002703 same experiment
	MGI:4823707 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source