EMAGE mouse gene expression spatial database

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EMAGE:7252

Ect2 ect2 oncogene ( MGI:95281)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004327. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004327_01	euxassay_004327_02	euxassay_004327_03	euxassay_004327_04

euxassay_004327_05	euxassay_004327_06	euxassay_004327_07	euxassay_004327_08	euxassay_004327_09

euxassay_004327_10	euxassay_004327_11	euxassay_004327_12	euxassay_004327_13	euxassay_004327_14

euxassay_004327_15	euxassay_004327_16	euxassay_004327_17	euxassay_004327_18	euxassay_004327_19

euxassay_004327_20	euxassay_004327_21	euxassay_004327_22	euxassay_004327_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7252_wholemount_strong.wlz

7252_wholemount_moderate.wlz

7252_wholemount_weak.wlz

7252_wholemount_possible.wlz

7252_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7252_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 moderate expression: see section 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 moderate expression: see section 14

olfactory cortex ventricular layer

strong

regional

strong expression: see section 09 10 14 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 03

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 moderate expression: see section 14 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5083
Entity Detected:	Ect2, ect2 oncogene ( MGI:95281)
Sequence:	sense strand is shown >T5083 CCACGCGTCCGCTCTCGAGCCATGGCGGCGCCTGTGAGGGGGAGACTTAGTGTTTGAGAAGGATAAGCGA GGACTGCGCCGTCGTGGTTCCTTTTTAAAATCTCTGAGCGTGCCTTCATCAAGTAGTGCAAACAATGGCT GACGACAGCGTGTTACCATCTCCTTCTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATTCTAAAG TTGCTGAAATGTCCAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAAGAAATGCCGCAGGT TGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAGACTATT AAGATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGAGGAAAAATTAATAA AAAGTATTATTAATATGGAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGAAAGTTT GGATTCCCCAGAATTTGAGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGACTTATAC AAGGCTGATTGTAGGATTGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCTTGCCGT TTTCCTGCCGGCCGCTGTATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAGGAAGAA GGAGGAGCTTGTCAAATTGGTGACGTTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGTAATTCC AAAGTAACACATCTGGTGGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGGGCACTC CAATTATGAAGCCAGAATGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGCAGCAGT TGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGATTTTCA GATGAAGAGAAACATAGTATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTGGGGATG AAAGGTGCACTCACCTTATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAAGAAACT TTTTGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACTATGTAT TTGTATGAAAAGGCTAATACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTCCAAACA GCAACCGCAAGAGACGGAGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTCTCCTTT CCCTCCCCGGAAGCGGCCCTCAGCTGAGCACTCTCTTTCTATTGGCTCACTCCTGGATATCTCCAACACA CCCGAGTCCAGCATCCACTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTACTCCAG TTCCTCCAAAGCAGTCAGCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTATGTCAA TATACTGGCCACAATCATTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGGCCTATT CTTGCACCTGAGGAGATCAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGAAGATCA AGGATGATCTTGAAGACCTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCTTAAATA TGCAAAAGATTTGGTAAAAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATGATCATT AAATGTGAGAAACAAAAGCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAATGTGGAC GACAGAGCCTTGTGGAACTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTTAAACGA TCTTAAAAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTAAAA GAAGTAATGACACATATCAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATGTTGTTT ATGAAGTAGATGGATGCCCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGAAACGGT TTCCCTTGGTGAGCACCCTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGCCTCGAG ATAGCAAGAAAGCGGCACAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCCCCGCTT CTCTGAAGCACATTCATCTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGACAGAAGA TTGTCACAATGCCTTTGCCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGCTTCCAG ATGACGTCAGAGGAGCTTCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACACCATTT GTAAGGCAGATGCTGAGAATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATAT GGATAGTACATTGAGTCGAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCATTCTCT TTTTCCAAAACTCCCAAGAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAAGGAGTC CTCCGAGCAGTGGCAAGCTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCCATCTCC CTCCCTTGTCAGCCTCCCTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACTCACTTG ATATGAAACTTAAGTCCTGTGGGACCTATGCTTCAACAAGACGCTGACTTAAGTGGTACTTTGAATTAGC ACTTGGTAAAGTTGTAGAGAAGATTAGTAACACTAACCAACGCCGTTTTATTTTCTTGAAATACAATTTT CAAGATGGTCTATGTAAAAAAAATATGGATTTTGATGTAATTTATCTTTATTTGAATCTCACATCTGAAG ACCAGTGACTTAAGTTATTCCTGATAATCAGTAGCTTTGTAACCCTGATACAGTAAACAGTTGGTGGGTG CAAAATACTATTGTGTATTTGTATAGTATCTTGAATTTTTGGAAGTACATATTTGTGTATCTCAATTTGT GCAAAGACTGAACAGCAATGCAACACTTGCTGAATAAAACCCTCTGATTAAATTCTAGAGGCTTGTTCAG TTCGATTGTTTGATGTACTTAACTGCGCTTTTGCAGTTTATTTTCTTTTAAAATAAGAGATCTAACACAT GAATTTTAAAGGATACACCATTATTAACCATACAATTACGATCGTTTGGAATTTTCTTTTGCAAATTGAG ATAAGAAGAGAAAGATTACAAAACATTGTATATGTTGCGAAGATAAGATATTAGTATGTACTTTATAATT GTTTCAGAGACTTTTATTTTTATTTTATTTTCTTATTCTAGACACTAGCAAATCTTGATTTAGTTTTGAC TACTGTATTGAATTTATTTCAAAGGATAAGACCACGTGAAAACTGTGTTAAGAATTGCTTCTGTTCTTTG TACAGTTCTGAAGTTGTCTTCAGTTTTGCTAATCGCCTGTGAAAAGCATAGATATAATGTGTGGTGAACT TTTTATTGTGGTCATATCATTAAAATAAGATTGGAAATTTATTGTCAAAAATTTTGTGAATCTGCCATTA TCTTCTACTGCAATATTTCTTTTAGTCAGTAATACAGAATGACTGGGCTACTATGGCCAAGAATATTTTA TTTCTTACACGTTCAGAGGAGTGTTACAGGTCTCCTTGTTACATAATTTTCAATAATCTGCTTTAATAGT TTAACTGACTGGATTTTTTTCTATACCATGTATGTACCAATTCACACGTTAGTAAATGACCTTGTTTTGC TATATTTTAAAAGCAGTTATATTAGCAAGAACTTTGTAAATAAATAGCTTTAAAATACAAGTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5322744 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5322744 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7253 same embryo
	EMAGE:7251 same embryo
	EMAGE:7250 same embryo
	EMAGE:7249 same embryo
	EMAGE:7248 same embryo
	EurExpress:euxassay_004327 same experiment
	MGI:4824468 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source