EMAGE mouse gene expression spatial database

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EMAGE:7320

Vstm2a V-set and transmembrane domain containing 2A ( MGI:2384826)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004356. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004356_01	euxassay_004356_02	euxassay_004356_03	euxassay_004356_04

euxassay_004356_05	euxassay_004356_06	euxassay_004356_07	euxassay_004356_08	euxassay_004356_09

euxassay_004356_10	euxassay_004356_11	euxassay_004356_12	euxassay_004356_13	euxassay_004356_14

euxassay_004356_15	euxassay_004356_16	euxassay_004356_17	euxassay_004356_18	euxassay_004356_19

euxassay_004356_20	euxassay_004356_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7320_wholemount_strong.wlz

7320_wholemount_moderate.wlz

7320_wholemount_weak.wlz

7320_wholemount_possible.wlz

7320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 04 05 06 14 15

knee mesenchyme

moderate

regional

moderate expression: see section 02 03 16 17 18

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 16 17 18 19 20 21

hypothalamus mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21

olfactory cortex mantle layer

strong

regional

strong expression: see section 10 12

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 13 14

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

ventral grey horn

strong

regional

strong expression: see section 06 07 08 09 10 11 12

mandible

moderate

regional

moderate expression: see section 02 03 04 05 15 16 17 18

maxilla

moderate

regional

moderate expression: see section 02 03 04 05 06 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5282
Entity Detected:	Vstm2a, V-set and transmembrane domain containing 2A ( MGI:2384826)
Sequence:	sense strand is shown >T5282 GACACTTCACGGCGTGGCAACCCGGGTCTGTGCCTTGAAGCCTCCGGATCGCAGCCAGCTCGGTCCATCC CTCACTAGTCGCAATCCCCTGTGTCCAAGCTACTCTTTGCTATGAGCGGCAGCATGCGTGCAGTATCGCG CCCCAGGCTCTGAGAGCAGCCTGCGGACACGCTTGCCTATCTGTCTTTTTAGGTTTTGGGGCTCTGGGCT ACACGGATGTGCCCCACTCCCTTGGCATGATGGGGATCTTTTTGGCGTCTGTTGGATTTATGTTCTTTTC CGTGTTATATGTACAACAAGGGCTTTCTTCTCAAGCAAAATTTACCGAGTTGCCGAGAAATGTGACTGCT ACCGAAGGGCAAAATGTGGAGATGTCCTGTGCTTTCCAAAGCGGCTCTGCTTCAGTGTACCTGGAGATCC AGTGGTGGTTCCTTCGGGGGCCAGAGGACCTGGAGCAAGGCACGGAGGCTGCAGGCTCGCAGGTGGAGCT CTTACCCGACAGAGACCCGGACAACGATGGGACCAAGATTAGTACAGTGAAAGTCCAAGGCAATGATATC TCCCACAAGCTTCAGATATCCAAAGTGAGAAAAAAGGATGAAGGTTTATACGAGTGCAGGGTGACTGACG CTAACTACGGGGAGCTTCAGGAACACAAGGCCCAGGCCTATCTGAAAGTCAATGCCAACAGCCATGCTCG GAGGATGCAGGCCTTTGAAGCCTCACCTATGTGGCTGCAAGACACGAAGCCTCGAAAGAACGCATCATCG GTGGTTCCCAGCAGCGTCCACAACTCTGCCAACCAACGAATGCACTCCACCTCCAGCCCTCAAGCGGTAG CCAAAATCCCCAAGCAAAGTCCACAATCAGGTGCGAGGATAGCTACAAGCCATGGACTTTCTGTCCTGCT TCTTGTTTGTGGCTTTGTGAAGGGTGCTTTGCTTTAATCTAAAGTGCTGACTTTTTTCCAATATCACTTT CTCTTCTTAAAGCAAAGAGCAAATCGCCTGTAAAATCTACGGAGCGGACAGCAAAGTTGACCCTATACTC CAAGCACCATTCTGCACCCCTGTACTCTAGTTATCTACACAAGGAGCATCAGCTTCCGGAAGCATAAGTG AAGACACTGTCACACGCTTTATTGATAATATTTTCTTTGGGAAGTTGCTGATCTTTTATTTCAAGAGAAT TAATGGGAAGAGATAGGACATTTTCCAATTACAAGACCAATTTTTTTCCTTTTATTTCAACAAATAAAAC CTGCATTTCACTGACTGCTCAGGAGTTGGCCTGAATGACATCAGTATACTAAATATTTCCATGGATTCCA CCAATTTCCTAACGAGGGACACCTAATCTTCAAGAAGCAAACAAAGATGGAAAACCTAAGAACCACAAAC TGTCTCATACAGCACCCCAGCTGAGGAACAAAACAAATAGCTAAATGCTGACCATGGCAAATCAACATCA GACAACTTTATTTTACATATGGAATAATCAAAGAAAGTTTTTTTTTACTTCCTTTTTGCCCCCTGGAATT TATCTTGGAGTTTCCCTTTTTTCCTTGATTGCCGTTTTCGTTCAATGGTAGCAAGTGCCAATTATGGCCA ATCCTTGTCAATCCTGGAAGGTTTATATTCATATACATTGAGTGTGGTATATATCAATGTATTTTAATTC ATTTGGCAATTTCTGTATAGGCAAACCTGGCAAATTCTGTAAATTGCTTATAGTATGTGTGATATGACTT CAAGGTAGATAGGCTATGATGCTCATGCAAGCTGACTTTCTTCATTCTATATACAAATATATTCATGAGC ATATATTAGGCCACCAACTTCTTTTCCTAAAGAATTATTTTTCATTTGTACCTCATGTATTTTGTGAATT TTGTAGTATATTTCTCTGTTCCACTAGTTTGACCGCTACAGTTTGTCTCTGTTGTCCTCTACTTCCTTCT GGAAAAATTTAAAATTGTGTATGTCTCTGATAAATGAATTAATTTTGTTGTGTGTATGCTATGTTGGAAT TTGCTGTGTTCTTTTAAACATGTATTTATTAAGGTTTGGGGATCTTGAGTTGAGTCTGAAGAATGCACAC CTGGTTTTTGACAGAGTTCCTCATGTTACCAATATTCTATCTCAGAGAAAGAAAGACACCAAGTGGGAAA ACTAAGAAGACATTTTGACTTCCCAAGATCCTGGAAGAGCACTTCACACTCTGACTAAATAATGTTGCTT TTTTTGTTCTTCAAGACTTTTTTGTAGCTTTGTCTTTCTGTTAGTTGCTGCTAATTATATTTTAATGTCT ACTAATTAAAAATTAAAATGTGATTGTTGGCTGAATACAATATGCAAATGACTGCAAAGCCCATACTGAA GAAAATAGATGTTTAATCTTCACTCAATAATTATAATTTTAAATAGTTCATCATTATTTTTTGACCTTAT GATATTTTGTTAGACCTGTTCTAATTACATCTTTCTCTGGCAAAGAAAGATAGAACAATCAATACATTCC CTCTTACAGTATGGAATGGTTGTGGCTTAAGAAAGAATGCATCCAGATGGTCTTCCAGAGAGATTATTTT ATTTTCATTATAAAACCAGAAACCATATATGTAGGAATGGTTCATTCCTAATGTAAGGCCATAAATTGTA GCTTGAAGGCAAGGAATACATTTGTTTTTTTATGGTAAAGGACTGGCCTCTGACATGCACTTATAAGCAA TGTGAATATTTTCATAATATGCTTGACATTCTCCTTTAACAAATATTGTTTTATGGTAAATCTTTCCTTG CCATTTTCCTTCTTTCATTTGATTCATTATTTCATTCTAATGAAGAAAATAAAGGTTTAATTATGATACT TTATTAACATACAAATGTATTTTCTTTCTAAGTTAAATATCTGAAAGTTGTATAAAATGATGGTAGAGAA ATATTACTCATTCGGTTTCTTTGAGCTTTAAGAATCCCATACATTGCAGTATATATTAGAATACTGATTT AACATCAAACTGGGGGGGAAAATCATGTATTATACTTTTACTCAATGTCTAGGTAATGAATTCAGCTAAT TTTACAGCAAGCCAAATGTGTACCCGTATCAGTAATGTTCACCATGCTTGTAATAAAAGGGTATATGCTA GTTTTGGAAGAATGCTCATTAGATTCATTGTATCAGTGTCCAAAATAATAAAGACCTGTTTATCAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5361611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5361611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7324 same embryo
	EMAGE:7322 same embryo
	EMAGE:7321 same embryo
	EMAGE:7325 same embryo
	EMAGE:7323 same embryo
	EurExpress:euxassay_004356 same experiment
	MGI:4829177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source