EMAGE mouse gene expression spatial database

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EMAGE:7463

Dalrd3 DALR anticodon binding domain containing 3 ( MGI:1915039)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005217. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005217_01	euxassay_005217_02	euxassay_005217_03	euxassay_005217_04

euxassay_005217_05	euxassay_005217_06	euxassay_005217_07	euxassay_005217_08	euxassay_005217_09

euxassay_005217_10	euxassay_005217_11	euxassay_005217_12	euxassay_005217_13	euxassay_005217_14

euxassay_005217_15	euxassay_005217_16	euxassay_005217_17	euxassay_005217_18	euxassay_005217_19

euxassay_005217_20	euxassay_005217_21	euxassay_005217_22	euxassay_005217_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7463_wholemount_strong.wlz

7463_wholemount_moderate.wlz

7463_wholemount_weak.wlz

7463_wholemount_possible.wlz

7463_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7463_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

choroid invagination

strong

regional

strong expression: see section 17 moderate expression: see section 06 07 08 09 10 11 14 15 16 18 19 not examined expression: see section 12 13

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 04

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 15 16 weak expression: see section 07 08

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 moderate expression: see section 06 07 08 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7586
Entity Detected:	Dalrd3, DALR anticodon binding domain containing 3 ( MGI:1915039)
Sequence:	sense strand is shown >T7586 ATGGCGACCGGGCGCCTTGGGGTTGGCGAGACGCTGGAGGCCCTTAATGCAGCTGTAGGGCCTGGTAGCC CAGTGTGGTTTAAGGAAACCCATGCCCGGCACCTTCGTGTTCGAGACTTCTTGGCACCGCGAAGCGCGCT ACAGGCGCGCTTCAGGGACGGCCAGGTGCCAGAGTGCGTGTTCCGTGCGGTCTCCTGCCTGCAGGGTCCC GGAGTGGCCCCTGTACTGCGCTGCGCGCCGACACCCGCGGGTTTATCGCTCCAACTACAGCGGCCCGCTG TCTTCGAACATGTACTCGGTGCCCTGGCCTCCTATGCTACTCCCGCCAAACCTGCCTCACCAGGCCCGCG AGTTGTCCTACACTGCCCAGCACTGCGCTGTAATTCGGGGACGCTCCGATTGAGCCAGCTGCGTGCCGTG CTCGTAGCCGATCATCTAGTACGGGTACTGCGTGCTCATGGGGTACGTGTGTGCTCAGTGCCCCCGGTGC GGGACCCTCACATGTCAACTTTCCTCCAGAAACTTCGGGTGGAATGGCCCACTGCCTCGAAGAGCACCTC GACCGAAACACTGAGAACCTGTGTTCTTGCAAACCTTAATGGTTCTAAGGAAGCGACGCTGCCCCCTGGC GTCCTAGGCCGACTGTGTCTGAAGGAACTGGTAGAACAGAGAGGCACTGCTGGCTATGACCCCAGCATAG ACCACTGTTTGGTGACTGAAGATGTGCTCTCTGTGCTCTCGGAGCTGCAGGAGGCTGTGCGCCACTGGCC AGAGGGCGGCCACCCAGGCCCGGCTGGGCGTCCAGATGCTGGTGTAGATGACTGCGTGGTTATACACGTG GTTAGCTGTGAAGAGGCATTCCAGCAACAAAAGCTGGATTTGCTCTGGCAGAAATTGGATGACCGGGCTC CTCACAAGCAGAAACACCTGGTGTGTGGTCCCGTGAAAATGGCTGGTGTACCCGGCACCCAGCTGACTGC CCCTCAGTACTACAGGCTCAGACATGCTCAGGTGTGCGAGGCCTCAGCTCTGAAGCACGGAGGAGACCTG GCACAAGACCCCGCCTGGACTGAGACCTTCGACATCCTCTCCGTGGCTACCATCAAATTTGAGATGCTAA GCACAGCCCCACAGAGCCAGCTCCTCCTGGCCCACAGCACCATCTCAACAAAGGGCACAAAGAGCGGGAC TTTTGTCATGTATAACTGTGCCCGCCTTGCCACACTCTTCGAGGGCTACAAACATGGAACGGAGCAAGGT CTGTATCCCACTTTTCCGCTTGTGAGCAGTCTGGACTTCTCACTACTCCATGATGAGGGTGAGTGGCTGC TGCTTTTCAACAGTGTCCTTCCCTTTCTGGACCTGCTGAGCCAGACTGTGAGTCTGGCCGGCACCCCAGG CCTCCACATCCCTGTCCGCACGGAGATGGTATGCAAGTTCCTGGTGCAGCTCAGCATGGATTTCAGCTCC TACTATAACCGAGTACATATTCTAGGGGAACCTCGGCCACACCTCTTTGGTCAGATGTTTGCTCGCCTAC AGCTTTTGAGGGCAGTGCGTGAGGTGTTCCACACAGGCTTGGCTATGCTGGGCCTCCCTCCGCTGAGCCA CATCTAAGGGCCCAATAACTGGGAATGTTTATAAAAGCATCAACTGGGAAAGAAACAAGACCTCACAGAT GTTGGGCACTTAAAGTCAGAATAAATACAGTTTCATCCTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242135 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242135 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7464 same embryo
	EMAGE:7461 same embryo
	EMAGE:7462 same embryo
	EMAGE:7460 same embryo
	EurExpress:euxassay_005217 same experiment
	MGI:4824229 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source