EMAGE mouse gene expression spatial database

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EMAGE:7508

C1ql3 C1q-like 3 ( MGI:2387350)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004547. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004547_01	euxassay_004547_02	euxassay_004547_03	euxassay_004547_04

euxassay_004547_05	euxassay_004547_06	euxassay_004547_07	euxassay_004547_08	euxassay_004547_09

euxassay_004547_10	euxassay_004547_11	euxassay_004547_12	euxassay_004547_13	euxassay_004547_14

euxassay_004547_15	euxassay_004547_16	euxassay_004547_17	euxassay_004547_18	euxassay_004547_19

euxassay_004547_20	euxassay_004547_21	euxassay_004547_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7508_wholemount_strong.wlz

7508_wholemount_moderate.wlz

7508_wholemount_weak.wlz

7508_wholemount_possible.wlz

7508_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7508_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 14 15 16 17

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 12 15 weak expression: see section 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04 20 21 22

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 07 16 17 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 16 17

ventral grey horn

moderate

single cell

moderate expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7542
Entity Detected:	C1ql3, C1q-like 3 ( MGI:2387350)
Sequence:	sense strand is shown >T7542 GGGCGCTGGGGGTGGTGATGGTGCTTCTGCTGGTCATCCTCATCCCGGTGCTGGTGAGCTCGGCCGGCAC GTCGGCTCACTACGAGATGCTGGGCACCTGCCGCATGGTCTGCGACCCCTATGGGGGCACCAAGGCTCCC AGCACCGCCGCCACTCCGGACCGGGGCCTTATGCAGTCCCTGCCCACTTTCATCCAGGGTCCCAAAGGCG AGGCCGGTAGGCCGGGGAAGGCAGGCCCGCGTGGGCCCCCGGGAGAGCCCGGACCGCCGGGCCCCGTGGG GCCCCCGGGCGAGAAGGGAGAGCCGGGTCGCCAAGGCTTGCCCGGCCCGCCTGGGGCGCCCGGCCTGAAC GCGGCCGGGGCTATCAGTGCAGCCACCTATAGCACGGTGCCCAAGATCGCCTTTTACGCTGGCCTCAAAC GGCAGCACGAAGGCTACGAGGTGCTCAAGTTCGACGACGTGGTAACCAATCTCGGAAACCACTATGATCC CACCACGGGCAAGTTCACCTGCTCCATACCGGGTATCTACTTCTTCACCTACCACGTCCTGATGCGCGGA GGGGACGGCACCAGCATGTGGGCTGATCTCTGCAAAAACAACCAGGTGCGTGCTAGTGCAATTGCTCAAG ATGCTGATCAGAATTACGACTATGCCAGTAACAGTGTGGTCCTTCACCTGGAACCAGGAGATGAAGTCTA TATCAAATTAGATGGTGGGAAAGCCCACGGAGGAAACAACAACAAATACAGCACATTCTCTGGATTTATT ATTTATGCTGACTGATAATGCAGAGACTGAGCTTGCTGTTCTGAGTTTGAACGCTGGATTCGTATGGCTA ACGTCAGTGAATCAAGGATCCCAGGGGATGCCCATTGCTGGGCATCTCAGTTGTGTAAATGTGGGGAGAT CAAATGCTACCTGACTCACATCTGTATCACTCAGACACATTATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240136 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240136 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7509 same embryo
	EMAGE:7510 same embryo
	EMAGE:7507 same embryo
	EMAGE:7506 same embryo
	EMAGE:7505 same embryo
	EurExpress:euxassay_004547 same experiment
	MGI:4823535 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source