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EMAGE:7509

Chst15 carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 ( MGI:1924840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509
"Pseudo-wholemount" of euxassay_004543. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004543_01 euxassay_004543_02 euxassay_004543_03 euxassay_004543_04
EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509
euxassay_004543_05 euxassay_004543_06 euxassay_004543_07 euxassay_004543_08 euxassay_004543_09
EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509
euxassay_004543_10 euxassay_004543_11 euxassay_004543_12 euxassay_004543_13 euxassay_004543_14
EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509
euxassay_004543_15 euxassay_004543_16 euxassay_004543_17 euxassay_004543_18 euxassay_004543_19
EMAGE:7509 EMAGE:7509 EMAGE:7509 EMAGE:7509
euxassay_004543_20 euxassay_004543_21 euxassay_004543_22 euxassay_004543_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7509Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7509_wholemount_strong.wlz
7509_wholemount_moderate.wlz
7509_wholemount_weak.wlz
7509_wholemount_possible.wlz
7509_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7509_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 02 03 04 07 19 weak expression: see section 20 21 22 23
hindlimb digit 1 metatarsal
moderate moderate
regionalmoderate expression: see section 20 21
hindlimb digit 2 metatarsal
moderate moderate
regionalmoderate expression: see section 03 04 20 21 22
hindlimb digit 3 metatarsal
moderate moderate
regionalmoderate expression: see section 03 04 20 21 22
hindlimb digit 4 metatarsal
moderate moderate
regionalmoderate expression: see section 04 21
hindlimb digit 5 metatarsal
moderate moderate
regionalmoderate expression: see section 04 20 21
telencephalon mantle layer
strong strong
regionalstrong expression: see section 19 20 21 22 moderate expression: see section 04 05 06 07
telencephalon marginal layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 03 04 05 06 07
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 08 09 10 11 15
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 10 17 18
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 17 18 19 20 21 22 23 moderate expression: see section 05 12 13 14 15 16
pons mantle layer
strong strong
regionalstrong expression: see section 09 19
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 14 17 weak expression: see section 15 16
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 weak expression: see section 19
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 19 20
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 17 18 19 20 21
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 18 19 21 22
oculomotor iii nerve
moderate moderate
regionalmoderate expression: see section 14 16 17
trochlear iv nerve
moderate moderate
regionalmoderate expression: see section 14 17
dorsal root ganglion
weak weak
regionalweak expression: see section 10 11 17 18 19 20
not examined not examined
regionalnot examined expression: see section 01 02 03 21 22 23
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 21 22 23
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11
hindgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15
rectum
moderate moderate
regionalmoderate expression: see section 12 13
midgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17 18
midgut loop
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13
mandible
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20 21
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 08 09 11 12 13
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 16 17
maxilla
moderate moderate
regionalmoderate expression: see section 05 06 07 08 15 16 17 18
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 08 09 12 13
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 07 16 17
bladder
moderate moderate
regionalmoderate expression: see section 14
bladder
moderate moderate
regionalExpression in the fundus region.
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 11 12 13
clavicle
moderate moderate
regionalmoderate expression: see section 09 10 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7538
Entity Detected:Chst15, carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 ( MGI:1924840)
Sequence:sense strand is shown

>T7538
GGCTGGGCGCGCGCTCCCGCGGAGTCGGAGCTGCAGAGGCTTCCGAGATCGTCTTGGGCTTTTCAGGTCA
CCTAGCATTGGTATGAATCTGCTCCTCAAGAAGAGAAAATATTCTGAAGGCATGTCCGTTTACCTGTCAA
CGAGGAACCCATAATGTCTTCTCATCTCCAGTGTGGCTCCATGCTCTCTCCTGATGCTACTCCAACTTTC
TTTGGTGCCATGGGAAAAACCTGAAGCACGAGCAGAGTCACCTCCAAATCTGTGCTCACTTCATTTTGTT
GCATGGCTGGTGCGGAACAGACTATTGGAATTTGCTCCTGTGTGTTGCCCTTCGTACCTGAACCAAAGAC
TGTGCCTCATTCTCTAGTGCATTGCTGCCTGCTGTCTTCCTCGTTCATTTCTGTTGCTTGTGCCTTCCTA
CTGTCTTCTGTCATCACAGGACACCCTTAAAGTTTCTCACTGGACTCCTCGAGGGCTGGCCCATGGCTCT
CTAAGCCAGCTGTCCAAGGTCTCTACCATGAGGCATTGCATTAATTGCTGCGTCCAGCTGTTCCCCGAAG
ACACACACAAACAGCAAGTTGCCTGCCAAGGAGGCCCCCATCACAGTCATCAGGCGTGCCCCACTTGCAA
AGGAGAAAACAAAATTCTGTTTCGTGTGGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGG
ACTGAGGGCAATGAAAACTGGGGTGGGTTTTTGCGCTTCAGGAAGGGGAAGCGATGTAGCCTGGTCTTTG
GATTGATAATAATGACCTTGGTGATGGCTTCTTACATCCTTTCCGGGGCTCATCAGGAACTTCTGATTTC
CTCCCCTTTCCATTATGGGGGCTTTCCCAGCAACCCCAGCGTGATGGATGGTGAGAACCCCAGTGATGTG
AAAGAGCATCACTACCAGCCTTCTGTAAATAACATCTCCTATGTAAAGGACTATCCGAGCATTAAACTGA
TTATCGACAGCATTGCTGCCAGGATTGAGTTCACAACCAGGCAGCTCCCGGACTTACAAGATCTCAAGAG
ACAAGAGTTGCATATGTTTTCTGTAATCCCCAGCAAATTCCTCCCGACTAGCAAGAGCCCTTGTTGGTAT
GAGGAGTTCTCGGGCAGGAACACCACTGACCCCTACCTGACCAATTCCTACGTGCTCTACTCCAAGCGGT
TCCGCTCTACCTTTGACGCTCTGCGCAAGGTTTTCTGGGGCCACCTGTCCCACGTGCAGGGCAAGCACTT
CCGCCTGCGCTGCCTGCCCCACTTCTACATCATTGGGCAGCCCAAGTGTGGAACCACTGACCTGTACGAC
CGCCTCCGGCTGCATCCAGAAGTGAAATTCTCAGCCATCAAGGAGCCGCACTGGTGGACCCGGAAGCGCT
TTGGAATTGTCCGCCTGCGGGACGGACTACGAGACCGCTACCCTGTGGAAGATTACCTGGACCTCTTTGA
CTTGGCTGCACACCAGATCCATCAAGGACTGCAGGCTGCCTCTGCAGAGCAGCCCAGCAAGATGAATAAG
ATCATTATTGGAGAGGCCAGCGCCTCTACAATGTGGGATAACAATGCCTGGACCTTCTTCTATGACAACA
GCACAGACGGCGAGCCACCATTCCTGACCCAAGACTTCATCCATGCCTTTCAACCAGAAGCCAAGCTCAT
TGTTATGCTCAGGGATCCTGTGGAGAGGTTGTACTCAGACTATCTCTACTTCGCAAGTTCCAATAAATCT
GCAGATGACTTCCACGAGAAAGTGACGGAGGCTCTGCAGCTGTTTGAAAATTGCATGCTGGATTATTCAC
TGCGCGCCTGCGTCTACAACAACACCCTGAACAATGCCATGCCCGTGAGGCTCCAGGTTGGCCTGTATGC
TGTGTACCTCCTGGATTGGCTGACTGTCTTCAGTAAGGAGCAGTTCCTCATTCTCCGTCTGGAAGACCAC
GCATCCAATGTCAAGTATACCATGCACAAGGTCTTCCAGTTTCTAAACCTGGGGCCTCTGAGTGAGAAGC
AGGAAGCTCTGATGACCAAGAGCCCTGCATCCAACACACGGCGTCCTGAGGATCGTAGTCTGGGACCCAT
GTGGCCCATCACCCAGAAGATTCTGCGTGAATTCTATGGGCCTTTCAACACAAGGCTGGCACAGGTCTTG
GATGACGAAGCATTTGCCTGGAAGACAACGTGAGAGCTGAGTCCCTTCTGCAGAAGCTGGGCCCACTGAC
TCTGTCATTGCCATGACTTTAAAAGTCTCCAGGCGGAGAACTGTCACCCACAGCGTGGAGAAAGCCGCTG
GAGCCCCACCCAATCCTCTTCTCATGTCCAAGGCACCTGGTGTGGGAACTATTTTAAAGCTTTCCTTGCC
ATGTTCAGCAGCTCTGCTCTTGATTGCAAGGCTCGTCCACCTGAAGCCTTGGAAACGACACTGGGGGTAG
GCTTCTGTCTGTTCCCAGAGTGCCTTGGATCTCAGGAGGGCAGTGGATCTCTGGCACCCAGTGGGTAGGA
AGAGGAAGCTACGGGAGGCCATAGACCAGCCTTGCCTTCCTTCCTCCAGGAGAGTTTGTCTGGGTGTAGG
GTTGGACAGCTCTGGGATCCGATTAAGGCCATGTTCTGGGGCTGCCCCTCACGTGTTATATCCCAGCAGG
CCTTGCATAAATCGACAACCCCATGTGGACAGAGAACAGAGCTGCTTGGAGAATGGAGGCTCTTTCGTTT
TTGTTCGTATTCCCAAGTCAGCCCTTCGGTGACATCAGAAATGAGCCCTGTTCCCTTTGCTGTGCTCAGA
AGAAACTCAGAAGTCAGCTTTTGGCAGTAGGCTCGTCTCTCTGACCTATTCTGCCCATACACTTTGTTTA
TGCCCAGCGTAGAGAAGGAGCGTTTTAAAACAAACTGCTTCTCGCATTTGACTGGCCCTGCCTCTCTTTT
CTGCTCCCTTGGATGTTTTCCTTAAGGACTTCAGTTTATATTTGGAAAGGGAAGTTTTGCTGTTAATGCC
AGAACAGAACACCTCAAGGCATCGAACAGTGGAAAAGGAAGGAAGTGAAGCCCTCCCCATTTGTGGGGGT
CACTGGAGAGTGTGGGGATGTTTTTGGTTCTGGCTGGTTACAGGCTCCCCCCTGTCTTCCTGTTGGAGGA
ATCTTTATCCACATTCTCTGTCATGTGCAGCCAAAGTTCTGTAACTCACTAAGGGTGAGTCTCAAGACAC
CCTTAGAATGTCCTCAGAAACACATCTCACAAACTAAGCTCGGGCCACATGTGGTGTCACTAGGAGATAG
CTTTGATTTTTATGAACCTTTCCAGGCCTGCTAGCAGCTTGGTTCGCAAAGGATATGCTGAAAGTCTTGT
CATTTTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4240286 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240286 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7508 same embryo
 EMAGE:7510 same embryo
 EMAGE:7507 same embryo
 EMAGE:7506 same embryo
 EMAGE:7505 same embryo
 EurExpress:euxassay_004543 same experiment
 MGI:4823867 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS