EMAGE mouse gene expression spatial database

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EMAGE:7575

Grhl2 grainyhead-like 2 (Drosophila) ( MGI:2182543)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004631. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004631_01	euxassay_004631_02	euxassay_004631_03	euxassay_004631_04

euxassay_004631_05	euxassay_004631_06	euxassay_004631_07	euxassay_004631_08	euxassay_004631_09

euxassay_004631_10	euxassay_004631_11	euxassay_004631_12	euxassay_004631_13	euxassay_004631_14

euxassay_004631_15	euxassay_004631_16	euxassay_004631_17	euxassay_004631_18	euxassay_004631_19

euxassay_004631_20	euxassay_004631_21	euxassay_004631_22	euxassay_004631_23	euxassay_004631_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7575_wholemount_strong.wlz

7575_wholemount_moderate.wlz

7575_wholemount_weak.wlz

7575_wholemount_possible.wlz

7575_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7575_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 14 15 weak expression: see section 11 12

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19

pancreas

weak

regional

weak expression: see section 07 08 09 10 15 16 17

thyroid gland

weak

homogeneous

weak expression: see section 10 11 15 16

adenohypophysis

moderate

regional

moderate expression: see section 13 weak expression: see section 10 11 12 14 15

vibrissa

strong

regional

strong expression: see section 05 06 07 19 20 21 moderate expression: see section 04 22

cochlea

strong

regional

strong expression: see section 05 06 09 16 17 moderate expression: see section 07 08 18 19 20 21 weak expression: see section 02 03 04

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 20 21 22 23 24

cornea epithelium

moderate

regional

moderate expression: see section 01 02 03 21 22 23 24

naris

strong

regional

strong expression: see section 11 12 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 14 15 16 17 18

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 11 14 15

esophagus

strong

regional

strong expression: see section 12 13 14

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

rectum

strong

regional

strong expression: see section 10

midgut

moderate

regional

moderate expression: see section 12

midgut loop

strong

regional

strong expression: see section 09 10 11 13 14 15 16 17 moderate expression: see section 12

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15 16

lower jaw molar

strong

regional

strong expression: see section 06 07 19

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

upper jaw incisor

strong

regional

strong expression: see section 10 11 14 15

upper jaw molar

strong

regional

strong expression: see section 06 18

bladder

strong

regional

Expression in the fundus region.

kidney calyx

moderate

regional

moderate expression: see section 05 17 weak expression: see section 06 07 08 09 15 16

urethra of male

strong

regional

strong expression: see section 10 11

larynx

moderate

regional

moderate expression: see section 12 13

left lung

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 weak expression: see section 03 04

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 moderate expression: see section 21 22

trachea

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7685
Entity Detected:	Grhl2, grainyhead-like 2 (Drosophila) ( MGI:2182543)
Sequence:	sense strand is shown >T7685 GGACGAAAGCAGAATTACCTGTAGCTCTTGTTCTGCCATCTCGGGCGCTCTCACACACCTTCACCTGCAC AGACTTGAAAGTCCAGTTTCACCAGAGGCTGAGGCTCCAGGAAAAGGGGAGCGAGTTCATTGGATCAAAC ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGACCCTCCCTTCA ACACCCGAAGAGCCTACACAAGTGAGGATGAGGCCTGGAAGTCATATCTGGAGAACCCCCTGACTGCGGC CACCAAGGCGATGATGAGCATCAACGGGGACGAGGACAGTGCTGCCGCCCTGGGCCTGCTCTATGACTAC TACAAGGTTCCTCGAGACAAGAGACTTCTGTCTGTGAGCAAAGCAAGTGACAGCCAAGAAGACCAGGATA AAAGAAACTGCCTTGGCACCAGTGAAGCCCAGATCAATTTGAGCGGAGGCGAGAACAGAGTGCAGGTTCT GAAGACTGTCCCGGTGAACCTCTGTCTAAGTCAAGACCACATGGAGAATTCGAAGCGCGAGCAGTACAGT GTATCCATCACCGAGAGCTCTGCCGTCATCCCCGTGTCAGGCATCACCGTGGTGAAAGCCGAGGATTTCA CACCGGTGTTCATGGCGCCCCCGGTGCACTATCCCCGCGCGGACAGTGAGGAGCAGCGCGTGGTTATCTT TGAACAGACTCAGTACGACCTGCCCTCCATAGCCAGCCACAGCTCCTATCTCAAGGACGACCAGCGCAGC ACGCCGGACAGCACCTACAGCGAGAGCTTTAAGGACGGCGCCTCGGAGAAATTTCGGAGTACTTCTGTTG GTGCTGACGAGTATACATATGACCAGACGGGAAGTGGTACATTTCAGTACACCCTGGAAGCCACCAAATC TCTCCGTCAGAAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAATTCTATGCCATAACACTC AGTGAGACTGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTGATGGTGGTCT TTAGTGAAGACAAAAACCGAGATGAGCAGCTGAAATACTGGAAGTACTGGCACTCCCGGCAGCACACTGC CAAGCAGAGGGTCCTTGACATTGCTGATTACAAGGAGAGCTTCAACACCATCGGGAACATTGAAGAGATC GCATACAATGCTGTTTCCTTCACCTGGGATGTGAACGAGGAGGCAAAGATTTTTATCACCGTGAATTGCC TGAGTACAGATTTCTCCTCCCAAAAGGGTGTAAAAGGACTTCCCCTGATGATTCAGATCGACACGTACAG CTACAACAACCGCAGCAATAAACCCATCCACAGAGCATACTGCCAGATCAAGGTCTTCTGTGACAAGGGA GCAGAAAGAAAAATCCGGGATGAAGAGAGAAAGCAGAACAGGAAGAAAGGGAAGGGCCAGGCCTCTCAAG CCCAGTGCAACAACTCCTCTGATGGGAAGATGGCCGCCATACCGTTACAGAAGAAGAGTGACATCACGTA CTTCAAAACCATGCCCGACCTGCACTCACAGCCTGTGCTCTTCATACCAGATGTTCACTTTGCAAACCTA CAGAGGACCGGACAGGTTTATTACAACACAGACGATGAGCGAGAAGGCAGCAGCGTCCTTGTTAAGCGGA TGTTCAGGCCCATGGAAGAGGAGTTTGGTCCAACACCGTCTAAGCAGATCAAAGAAGAAAACGTAAAACG AGTGCTTTTATATGTGAGGAAGGAGAACGATGACGTCTTCGATGCTCTGATGCTGAAATCACCCACGGTG AAGGGTCTGATGGAAGCGCTGTCTGAGAAGTATGGGCTGCCAGTGGAGAAAATCACAAAGCTTTATAAGA AGAGCAAAAAGGGCATCCTGGTCAACATGGATGACAACATCATTGAGCACTATTCAAATGAGGACACCTT CATCCTCAACATGGAGAGCATGGTGGAAGGCTTCAAGATCACGCTGATGGAGATCTGAGCCCTGGGTGTC CCCTCGATAGGAGCTTTTGGTATACTCCTTCCTGGGAGAGATGGGATCTCTGCCGCCCCAGGACCTGGAG ACCCACCCATCTCACTCACCTCTCAAGACTGTTACAAGACTGCTGGGAAGGGGGCAGGGCCCAAGGCCCA GTAATGGACTTCCTTCAACTCTTCCACTTGCTCCCTATGGAGCTGAAGCCTGAGCCCCTCAGCAAATTTC TTTGAGCCTGGTAGGCCCTTATTTATTGCCCATTTCCCTCCAAGAGCCCTCCAGGACTCTGCAAGTTACT GATAGCTCCATGTGAGACTTCCAGAGTTCCCCCTTCAAGGGAGATAGCAAGTCCACTCATCTCGAACCTG GGGAACTGGTGTGTTTTTTTTTTTCCTTACCTCTCCACATTTCTTGAGTGGTTGGGCCAGATGAGCTGCT TCTTGGTACAGTCTTCATGGATGGGGTAAGAAGGACACCAGTTTGCTGGCCCCAAACCTTCGGATTGCTC TGAGGAAGGTCATTTTGCTAAATACCTCCAGGAGACTCTCAGCAAATAGCCACTGGGCCTTGTACAGGAA GATATCGTGCTACCATGTCAGTAATAAGGAACCCGAGTATGCCTGATGACCATGTACATAACTTATACAA AATATTGCAATAATATATTTTACCTATGGTTGGTGGGCATGTTTACTGCCATTGGCCTGGAGGAGACATA CCTGGAGAATCAGAATGTTCTACTCTATCCAGAGGTGGTTAGGAGAGTGGTCTTTGGGATGTCATATGAT GGTGGTGAATGCCAGGATGGACTCTCCATTGGGCCATGCCTGAGGACTTGTTTCCCCAGAGCCCTCTTTC CTCAAGCATGACTCTCCCTGGAGGCCATTGTGGGTTTTTTGGTGATATATTGTCTCAGCACGCCCTTTGA GCTGTCTGGGAAGGGCCGTACTGTTGGTGCCTTCTCTAGCTTTGGTGTGGGGCATCTCTTGAGCCTTGTG CAAAGTCTCTGCAGAGGTTTGCCTCGGGGCCCTGTCACTGAGACCTGCTGCAGAGCTGCTTGGCCGACGT GGGGATCCTGGACACTGTAGTGCCTCATAGTTTACAAGCACGTCCTTCATCTCAAGTGGCCCCTTTAAAA ACCCACCTGCAGCTTTGTGAACTGAGCCAGCGGCCTTGCTTCTGATGGGAAGAAGTGAGGATGTCCACCC TGGCTGTGGGAAGCGTGGAAGCCCTTGCACTTCTGAAACCAACTCTGGGAGGTGTGCTGGAGAGCTAAGG CTGTCAAGAGAAAATGGCTTACCTGAACAGTCACGCAAGCATGCCCCATGGGCCTCTGTTCTCTTTGAGA GAACAGGTCAATAGTTCTGTTTTAAGGATTGGTATGTGCCCCTGTGGGCCGAGCACTAGTTTGGATAGCC AGGCACATCCCATCCGAAAGCCTGGATAATGGAACAGCTTCTATGGAAAGGTCTAAGTTTGTCTGCGAAG CACTTTGATGTTTTACCTGGAGGCTCTGCTCTCCGGCCCCTGTCTTCTTGCTCTACCTGTCCCGAGGCCC AGAATAGCTGAGGCTGACTCCAGCCAAGCTCTTGCTCTTCAAAGCTGCAGAGAAATTGAGTGGGTTGGAG AGTAGGTGCCGTAGCCAAGAGGATCCCAAAGGCTAGCAGGGGCACCTTTCTGAGAGCCCACTGGACTCCT TATCCCTCTTTGTGTTGCCAAAGGTTTGCATCCTGAGCTATGCTCTATTCCAGCCCCGCACTCCAAATGC CGCTCCTTGAAACTAGCAGTCTAGGGACACTCCAGGGTACGAGTGGGCCTCTGGTCTAAGATGGACAAAC TCTGCTGGAAAGATATGCTCTATGCGCTCCCTGTGCTATAGCACTTGGGGCTAGCTGCAAACGTGATTGG CAGACCTTAGAGGTGGGCTCAGGATTCCAGAGTAGGGAGACAGATCTGTAGCAACCTGACAGGCTACCAG CTCTGGAAAATTGCTGGTCTGCTCTAGGGCAGAAGACAAACCAGAGCCAGCGTTCCCTTTGTCCTGAGTC CCTACAATCCATGAGATAGCCATTGCCAGAACAAGGGATAGGAAGCCACAAAGCATCAGGACAGTGTCAG GTCCAGGATGTCTGCTGGTGGACTTCCATGTCGCTGTGCTGTCTGAGCTCTGCTGTGCCTCGTTTCCTAA ACAAAAACACTCCAGACACATAGAAAATGCTTGCCTTGCCCTGAACTAATCTCACCCAAACAAAGAATTT TTTTTTTTTTTTTGGTAATCTATAAAAGATGAGGGACACATGAAGCAAACATTTCATTCTATGTCCCCAA TTGTCAAAACTTCCTCAGTGTTTAGAAATTGAACCCAATAGAAAAATACTGAGTGAGCTGTCATCTTGAG GTTGGTTCCTAAAAGACTAAGGCACATCCCAATGTAGAAACCTGGGAAGTTTGAAATGAATTAAAACATT TTTCCCCTCATATTTTAATAGTCATAAAAATGCAACAGGAAATAAGGGTTTGTGCCAAATTCTAAGAATG GCCCTTTCTGAAGGACAAGCAGGGGAGACTCGTGTGTACAATCTGTTCCCACATTAAAAAAAAAAGAGTA AATGTAACTTAATAGTTTTGTAAATGGGAGAGGGGGAATCTATAAACTATAAATATGTTATTTTATTTTT TGTACATTTTTAAGAAGAAAAAATAAATATTCACAACATGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6306106 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6306106 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7574 same embryo
	EMAGE:7573 same embryo
	EMAGE:7578 same embryo
	EMAGE:7576 same embryo
	EMAGE:7577 same embryo
	EMAGE:7572 same embryo
	EurExpress:euxassay_004631 same experiment
	MGI:4825223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source