EMAGE mouse gene expression spatial database

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EMAGE:7588

Vat1l vesicle amine transport protein 1 homolog-like (T. californica) ( MGI:2142534)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004685. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004685_01	euxassay_004685_02	euxassay_004685_03	euxassay_004685_04

euxassay_004685_05	euxassay_004685_06	euxassay_004685_07	euxassay_004685_08	euxassay_004685_09

euxassay_004685_10	euxassay_004685_11	euxassay_004685_12	euxassay_004685_13	euxassay_004685_14

euxassay_004685_15	euxassay_004685_16	euxassay_004685_17	euxassay_004685_18	euxassay_004685_19

euxassay_004685_20	euxassay_004685_21	euxassay_004685_22	euxassay_004685_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7588_wholemount_strong.wlz

7588_wholemount_moderate.wlz

7588_wholemount_weak.wlz

7588_wholemount_possible.wlz

7588_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7588_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forearm mesenchyme

moderate

regional

moderate expression: see section 22 23

upper arm mesenchyme

moderate

regional

moderate expression: see section 23

hand mesenchyme

moderate

regional

moderate expression: see section 20 21

foot mesenchyme

moderate

regional

moderate expression: see section 03 04 05 18 19

knee mesenchyme

moderate

regional

moderate expression: see section 01 02 21 22 23

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 19 20 22 23

head mesenchyme

moderate

regional

moderate expression: see section 03 04 05 08 09 10 13 14 15 16 17 18 19 20 21 22 23

body-wall mesenchyme

moderate

regional

moderate expression: see section 03 04 05 08 09 10 12 13 14 15 16 17 18 19 20

adenohypophysis

moderate

regional

moderate expression: see section 09 10 11 12 13 14

vibrissa

weak

regional

weak expression: see section 03 04 05 06 18 19 20 21

hypothalamus mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 12 13 14 15 16

diencephalon lateral wall mantle layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

lateral ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23 weak expression: see section 10 11 13 14 15 16

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 09 weak expression: see section 10 11 13 14 15 16

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 09 weak expression: see section 10 14 15

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate mantle layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 12 13 14 15 16 17 weak expression: see section 09 10 11

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15

facial vii ganglion

strong

regional

strong expression: see section 05 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 07 16

trigeminal v nerve

moderate

regional

moderate expression: see section 15 weak expression: see section 08

ventral grey horn

moderate

regional

moderate expression: see section 08 09 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 12 13 14

cervical ganglion

moderate

regional

moderate expression: see section 08 15

thoracic ganglion

moderate

regional

moderate expression: see section 10

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8629
Entity Detected:	Vat1l, vesicle amine transport protein 1 homolog-like (T. californica) ( MGI:2142534)
Sequence:	sense strand is shown >T8629 CCACATTCAACAGGAGGAACCCGCGGGAGAGCAGACCCGACCCCTTCACAACCGCAGCCTGTGCGCCCCG CGGCCCCTCGAGCGCCATGGCTAAGGAAGGCGTAGAGAAAGCGGAGGAGACCGAACAAATGATTGAGAAG GAGACCAGCAAGGAACCTGCTGAGGGCGGCGACGGCTCCCACCGCCTCGGGGATGCCCAGGAGATGCGCG CTGTGGTGCTGGCCGGCTTCGGCGGACTCAACAAGCTGAGGCTCTCTAGAAAGGCCATGCCGGAGCCACA GGACGGCGAGCTCAAGATCCGCGTCAAAGCCTGTGGCTTAAACTTCATTGACCTGATGGTGCGACAGGGC AACATTGACAACCCTCCCAAGACCCCCTTGGTGCCAGGATTCGAATGTTCTGGGATTGTTGAAGCTCTGG GGGACAGCGTAAAAGGATACGAGATTGGAGATCGTGTCATGGCATTTGTCAACTACAATGCCTGGGCTGA AGTGGTGTGTACTCCAGTGGAGTTTGTCTACAAGATCCCAGATGACATGAGTTTCTCTGAGGCTGCTGCT TTCCCCATGAATTTCGTCACCGCATACACGATGCTCTTTGAAATTGCCAACCTCCGCGAAGGCATGTCCG TGCTCGTGCACTCAGCCGGCGGTGGAGTGGGTCAAGCTGTGGCTCAGCTGTGCTCTACCGTCCCCAATGT GACCGTCTTTGGAACAGCCTCTACTTTCAAGCATGAAGCCATCAAAGATTCGGTGACCCACCTCTTTGAC AGAAATGCAGACTACGTGCAGGAAGTAAAGAGGATCTCAGCTGAAGGCGTGGACATCGTTTTGGATTGCC TGTGTGGGGACAACACTGGCAAGGGGCTCAGTCTCCTCAAACCTCTGGGGACCTACATTTTATATGGTTC ATCCAACATGGTAACTGGAGAGACCAAGAGTTTCTTTAGTTTTGCAAAGTCATGGTGGCAGGTGGAGAAA GTGAACCCCATCAAGCTGTATGAAGAAAACAAAGTCATCGCCGGATTCTCACTCCTCAATCTGCTCTTCA AGCAGGGCCGCTCAGGCCTCATCCGAGGGGTGGTAGAGAAGCTCATCGGGCTGTACAACCAGAAGAAGAT CAAGCCAGTGGTGGATTCCTTATGGGCCCTGGAGGAGGTAAAAGAGGCCATGCAGCGCATCCACGACCGA GGGAACATCGGGAAGTTAATTCTGGATGTGGAAAAGACGCCGACCCCGCTGATGGCCAATGACAGCACAG AGACCAGCGAGGCAGGGGAGGAAGAAGAGGACCATGAAGGGGACAGCGAGAACAAAGAGAGGATGCCCTT CATCCAGTAAGCCAGACCTCGCATCGGTGAAAGTGGAGGATGGCTGGGAAGAAGATGGCCGCCAGGAGAA CTCTTCTGTGCCCCAGTGAACCAATGCTGTAGTCCAGTGTGTGTCGTGTTTGTCTGCAGTCAGCCAAGCT AAGAAGCTGTTGATCACTGTTGTGTTTGGAATTAAGTGCCAATCCCATTCCATGCAGTATTGTGTCCCTT CCTGGTCTGTGTATCACGCTCTCCCCTCTCCCTTAAAGCAAACCCATCCTTCTTTGGGTCCTTCTTGACG GTTCTAGAATAGCCTTGCAAACTAATACTGGCCCACAGGGCCCAAGGTCAGAAACCAGGCCACGCTTGAT TGAAAGGTGTTGTCACGGAGCTCTGTTCAGATCCCCACTCCTCTTCGGGCCAATGACACTCTTTGCCTGC CAACAATCAGCCATGCCTCTAAGCATGGCACTTACCAGTTTGGCTAGTGACAGGAGAGTAGGCCAGAAGT ATTCACGACCCTATACATACAGATTTCTCCAAGCAGTTACTTTGTCACTTTCCCTCTTAAGTGCTACGTC CCTGCGTGTGTCTCTGCACCTTACGGTCTGAGCCTCTTATTAACCCTCTTCTCTGGTTCTTATTTATAAA CCTAGGGAGGCAGATGTGGGTTCTTTCCTGGATAGAGGTGGTTCAACAGCGTCTGTCTAAGGGAAAGCTA CAGAGATCTCTCCTCTGAAACCATATCCCATGAGCAAAGTCCCCCCTGTGCCCAATGTAGACCCAACTCA GATGTCATTAAACTTACCTGGGTTCTCACTCGCAGCCTTTGTAGTTACCAACTACTATTTTAAAGCTCCT TCCCTGGAAACGCCTTTCCCAAGGAAGAGCTCCCTTGAAGACAGTATTTGGGGTTGAAAAGTCAACTGGT TTCTCTTGCCTATTCAAAGCCCTTTCTAATGTGTAACACACACGAGGGACACAGATGGTGGCTGCCCATC ATCTGACGACAAGTGCATGATCCTCCAACACACAAAACCATGGTGCTGATTTGCAGGTTCCTTAGAGGTT GGAGGCCAAACTGCTCAATCAAATACCCTGTTCACCATTCCAGCTTGAAAGGAAACAATTCTTGTCCTCT CTAATCGGCTTTGGAAGTAACACCAGTTACCACAGAGGAAGAGGGGTCTCCGCTCTCTGCAGTGAGGCTG TTTATTGCCTCCCTCCCCAGCCCTTCTGTCCTAAGAGGTCACAAATCCGTCACTCACAAACAGAGCTAGA TATAAAAGGCACCCCTGTAATGAGCACCCCTGAGCTTATTTTTGTCTTCTGGAAAACCCACGTGGGGTTC CCATGACCTTGGGTTGGTGTTCCAAGTTTCTAAACGTCCTTGAGGACTTAAGAGGGCTCCAGTGTTGCCC ACTTTTTTCTACTTACCACTTCAGGGTAGCCACCATTTTCCTGTGTGGGCTGGGAAGGAACTTGTCTCCA CAGTTCCCCCGCTTGGTTTGCAAAGTAGCCACTCACCTGTCATTTGTCGTGTTCCTTTATCTCGATCCTG ATCTTGTGATGATGACTTCTTCATGCCAAATATTTACCTGAAGGGAGTCTTTCCTCTCCGTGACAGGGAA AGTCAAATATGAAGATGAGAAAAGAAGCTACCAGCCAATAGGCCAAGGGGTGAGAATATGCGAAAGAGTG CCAGTCAGATTCTTATTTTCCATCCCTGGGGAGAGATGCTATTAACCATGTAACCAGTCGCATTTCCACC ATAAGCATGCTTGAGGATGGGTAGACCTGAGGACCACTCTCTGAGTGCTATTACAAGTATGTAGAAATAG CCTTTTAGAAAGGCTAAGATTAGAGTCAGGAAGGTTCGCCTTCAGGAGACACAGAACCTGACCCACATTC GGAATCCAGATAGTCTTGTCCTGTACAACTGAGAAACCTTGGGACGTTATCTCTCCCCAAACCTGTTTGG TTTGTTTCATTCTGGAAAACCATTGATCACACATGAAAACCCTCTCCCTCTCCCGAGAACACAGAAAGAG AGCATCTGAGGGTGAGCAGCGGCACCATGAAGAACAGGAGGGTGGGTGGGTGAGGGACAACCGGAATCAG TCAGTTATCCCGTGGACCTTCCTTCCCTCTCGTGGTTGTTTCCTGGAGGAAGTGTAAAGAGTTTATGTTT GCCTTAAGGGAGCTTCGAAGGCTTCTGGGAAGGTCGAGCTTTACTAGAACGAACCGAACATCGTCTAATG TGTCTGTGGCTTCAGAGCTTTCGTTATATTGTTATATTGTGCCTATAAAGCAAATTATGTTTACATAAAA CTATAAATAAAGTATTCAGCATAGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5719211 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5719211 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7586 same embryo
	EMAGE:7587 same embryo
	EurExpress:euxassay_004685 same experiment
	MGI:4829150 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source