EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7679

Cpne4 copine IV ( MGI:1921270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005273_01	euxassay_005273_02	euxassay_005273_03	euxassay_005273_04

euxassay_005273_05	euxassay_005273_06	euxassay_005273_07	euxassay_005273_08	euxassay_005273_09

euxassay_005273_10	euxassay_005273_11	euxassay_005273_12	euxassay_005273_13	euxassay_005273_14

euxassay_005273_15	euxassay_005273_16	euxassay_005273_17	euxassay_005273_18	euxassay_005273_19

euxassay_005273_20	euxassay_005273_21	euxassay_005273_22	euxassay_005273_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7679_wholemount_strong.wlz

7679_wholemount_moderate.wlz

7679_wholemount_weak.wlz

7679_wholemount_possible.wlz

7679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

regional

strong expression: see section 15

epithalamus

strong

regional

strong expression: see section 08 09 11 12

hypothalamus

strong

regional

strong expression: see section 10 moderate expression: see section 09 12 13 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 05 moderate expression: see section 06 07 09 13 14

cerebral cortex

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 11

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 10 11 14 15 16 17 18 19 20 moderate expression: see section 09 12 13

olfactory lobe

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 20 21

telencephalon ventricular layer

strong

regional

strong expression: see section 19

medulla oblongata

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

metencephalon

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

midbrain mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

strong

regional

strong expression: see section 02 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 03 04 05 06 16 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 07

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 16 17

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8925
Entity Detected:	Cpne4, copine IV ( MGI:1921270)
Sequence:	sense strand is shown >T8925 GCGAGTTCCTGAGCCCAGCAGCTCCTTCCACGCCGTTCACCCTCTCCCCCAGCCCCCAGCCCCCAGCCCC AAGAATGGAGTCGGGGTTTCCAGAGCGACCAAACTCTCCTCCCAGCATCTTTATCCTCCTCGGCTGTTGC TAAACTACCCCCAGTGGACCAAAGAGGGGGGAGGGAAGCCCGACTCTCCTAAGACCTCTTTACAACTACA TCACCCAGGACCTCCACTCCAAAGGCGGATTTTGCATCCTGGGGGCGTGACAGACCTGTCCCGGGCTGAA TTCTCTTTCTAGTCTCTGAGGCTGGCACACCCAGAATGAAGAAGATGAGCAACATTTATGAGTCGGCTGC CAACACGCTGGGAATCTTTAACAGCCCCTGCCTGACCAAAGTCGAGCTGCGTGTGGCATGCAAAGGCATT TCCGACAGAGATGCCCTGTCCAAGCCGGACCCTTGTGTCATCCTCAAGATGCAATCCCACGGGCAATGGT TTGAGGTGGACAGGACGGAGGTGATTCGTACCTGCATAAACCCAGTGTACTCAAAGCTGTTTACAGTGGA CTTTTATTTTGAAGAGGTCCAACGTCTCCGGTTTGAAGTTCATGACATCAGCAGCAACCACAATGGACTG AAGGAAGCTGACTTCCTTGGTGGCATGGAGTGCACACTTGGCCAGATTGTCTCACAGAGAAAGCTCTCCA AGTCCTTGCTGAAGCATGGGAACACAGCGGGGAAATCCTCCATCACGGTGATTGCTGAAGAATTATCGGG CAATGACGACTATGTTGAGCTTGCATTCAATGCACGGAAATTGGATGATAAGGATTTCTTCAGTAAATCA GACCCATTTCTGGAAATTTTCAGAATGAACGATGACGCGACTCAACAGCTTGTGCATCGAACAGAGGTTG TGATGAATAACTTAAGCCCAGCCTGGAAGTCATTCAAAGTATCGGTAAACTCTCTATGCAGTGGAGATCC AGACCGCAGGCTGAAGTGCATAGTGTGGGACTGGGACTCCAACGGCAAGCATGACTTCATCGGAGAGTTC ACCTCCACCTTCAAGGAGATGAGAGGAGCCATGGAAGGGAAACAGGTACAGTGGGAATGCATCAACCCCA AGTACAAAGCCAAGAAGAAAAATTACAAGAACTCAGGCATGGTGATTCTAAACCAGTGCAAGATCCACAA GATGCATTCCTTCTTGGACTACATCATGGGCGGCTGTCAAATCCAGTTTACAGTAGCTATAGACTTCACT GCCTCTAATGGGGACCCCAGAAACAGCTGTTCCCTGCACTATATCCACCCTTACCAGCCCAATGAGTACC TAAAGGCCTTGGTGGCTGTGGGAGAGATTTGCCAAGACTATGACAGTGACAAAATGTTCCCGGCTTTTGG GTTTGGCGCCAGGATACCCCCGGAATACACGGTCTCTCATGACTTTGCAATCAACTTTAATGAAGACAAC CCGGAGTGTGCAGGAATTCAAGGAGTCGTGGAAGCCTATCAGAGCTGTCTTCCCAAGCTCCAGCTTTATG GTCCCACCAACATCGCTCCCATCATTCAGAAGGTTGCCAAGTCAGCCTCGGAGGAGACAAACACCAAGGA AGCATCGCAATACTTCATCCTGCTGATCCTGACAGACGGGGTCATCACAGACATGGCTGACACCCGGGAG GCCATCGTCCACGCCTCCCACCTCCCCATGTCAGTCATCATCGTTGGGGTGGGGAACGCCGACTTCAGTG ACATGCAGATGCTGGACGGTGACGACGGAATTCTAAGGTCACCCAAGGGAGAACCTGTCCTTCGAGACAT CGTCCAGTTTGTGCCCTTCCGGAACTTCAAACATGCATCGCCAGCTGCCCTTGCCAAGAGTGTGTTGGCT GAAGTCCCAAATCAAGTCGTAGACTATTACAATGGTAAAGGGATTAAACCAAAGTGCTCATCGGAAGTAT ATGAATCGTCCCGGACACTGGCACCGTGAACTACACTTTTACAGAATTCTGAAGCACTGTTTATCTATTC CTGTACTTTAAATAACAACAAAAACAAAACAAAAAAAAACAATAATATACATTTAAAGATAGCACGTTTT GGTGATTTTTAATTAACTGACTTTTTATTTTATTTTATTTTTTGCATGTGTAGCCCAAGGCCTGGATCTG TTAAACCCTTGAATTGTTAAAGACTTTTTACAATGAAATACAGTTATAACACACTCTTTACAGCATGTCG CCTTGAAATTAAAATGGTATATGTATCCATTTTTATACCGGTTTGTTGAAATTTTGCTAAATTTCTTATT ATCTTTACACTGTAAAGCATTTTGAAACATTTCGTGAATGTCGATAGCCAAAACGTATATTTGGCTTAAT CTCCTATAGGATGAGAGACTTTTCAAAATGGCAGATGCATGGACTGTATTTTGCATGTTTAAAATAATTA TAATAAAAATAACCTTACACTGTTTTTGCAGTTGTTATCTATAATCTCTCCCTGCTGAGAATGGTCTCTC TCTACTAAATAAATTTATGTACCTAGAGGTAGTCTTTTTTGAAGCGGGCCTGGGTTATTTATCCCAGTAC CCATCTTTACACACTCCCATTGACACAAATTCCTGGACAATCTTGTTCTAATGAAGCTTTCTAATGCGGG GCCTGAGGTTCTGCACATCTCACAAGGTCCTCAATGATGCTAGTGCATCCCTTGCATGGCATGAGGACTA ATGGGGCTCTGAACTTGAGGCTTTCACCTCAGAGATCATGCCAAACAGAACAGCTCCTAGCCTGCTTGCC CATGGCACCTCTTCCACCCACCAGGCTCCCCAGTAGCTTAGAGGACCAGCCTGTCTCCAAGATGAGACTA GAAAGTAAGGAGACTGATTCCAGAAGCTGTCCACCCGACATCCCGGCCTCGCTGTTTTATAGCCCCACCT CTTCCTCATCCGGACTGAGGAGCACGCCTTCTGGTTTGCTCCTGCTTGGCTAAAAGGGGCAGCACAGCTC TCTGGATGGGTGGTTCCATTTTTTATGCCAGTTCCTAGAAATTATTTCCCAAGATTCCTCCCTTGGATCT TTGAGTGGGATCCTCTTCATGCCAAATAGCAACATGGCTGTCCAACTTTCCGTTCTAATCGCTTGGTCTT TGAGCTCACTGTGGGGGCCTTTTTTGACCTCTTTTGTATCACTCCTGAGTACAATTTCCATAAGCAGAAG TGCCCTGGAATTGTGTAGGGTGGAGATATTTTGTCTGAATCTAAGGAAGTCTAAAACCAAAATAATGACT TCCTCTTCTCTTATGATATTCTCTCCGAGAAATGGACAAATGTTTTATAAGCTATAAAAGGCACAAAATG TAACTTATTTTTGGTCAGAAAAATAGAGAAGTGTATCCAAAGCAAAAGAAATCTGTTGTTGGGGTAGTTT GGGTTCTTTTTCTTTTTTTTTGAAGCGGAAACAATAATAATTAATTAAGCAAATCAAATGTCTTGTACAA TTTCCAGAGTTATAATTATAGATTATCTTGCAAATACATGTCAACCAAATGCATAACATTTTGGGAATGA TGGTTTAAAAAATGTACGACAGAAAAAAACAGTCACAAATGTTTAAATTGTATTGTTTTCTGAATAAATT GGGTACTTACAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417611 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7681 same embryo
	EMAGE:7680 same embryo
	EMAGE:7678 same embryo
	EurExpress:euxassay_005273 same experiment
	MGI:4824031 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source