EMAGE mouse gene expression spatial database

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EMAGE:7692

Accn4 amiloride-sensitive cation channel 4, pituitary ( MGI:2652846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005297_01	euxassay_005297_02	euxassay_005297_03	euxassay_005297_04

euxassay_005297_05	euxassay_005297_06	euxassay_005297_07	euxassay_005297_08	euxassay_005297_09

euxassay_005297_10	euxassay_005297_11	euxassay_005297_12	euxassay_005297_13	euxassay_005297_14

euxassay_005297_15	euxassay_005297_16	euxassay_005297_17	euxassay_005297_18	euxassay_005297_19

euxassay_005297_20	euxassay_005297_21	euxassay_005297_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7692_wholemount_strong.wlz

7692_wholemount_moderate.wlz

7692_wholemount_weak.wlz

7692_wholemount_possible.wlz

7692_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7692_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

regional

strong expression: see section 17

hypothalamus

strong

regional

strong expression: see section 08 09 10 13 14 15 16

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

diencephalon roof plate

strong

regional

strong expression: see section 09 10 13 14 15

thalamus

strong

regional

strong expression: see section 08 09 10 11 13 14 15

cerebral cortex

moderate

regional

moderate expression: see section 01 02 03 04 05 06 13 17 18 19 20 21

telencephalon mantle layer

strong

regional

strong expression: see section 12 15 16 17 18 moderate expression: see section 01 04 05 06 07 08 09 10 11 13 14 19 weak expression: see section 02 03

olfactory lobe

moderate

regional

moderate expression: see section 13 14 15

hindbrain

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 07

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain roof plate

strong

regional

strong expression: see section 12 13 not examined expression: see section 14 15 16

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13

dorsal root ganglion

strong

spotted

strong expression: see section 05 06 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8958
Entity Detected:	Accn4, amiloride-sensitive cation channel 4, pituitary ( MGI:2652846)
Sequence:	sense strand is shown >T8958 GTCCTCCTCCTGGTTGCCCTGAGTTTAGAAGAGCAGCCGCTGCCGCCACCACCACTCCGGAGGGCACCAG GGCTGCTGTCCAGGGAGGGACAGTAGCAGTGAGGCTCTGGCCAGTCCCAGCAGCCGGGGACAGATGCCGA TCGAGATTGTGTGCAAAATCAAATTTGCTGAGGAGGATGCAAAACCCAAGGAGAAGGAGGCAGGGGATGA ACAGAGCCTCCTGGGGGCTGCTCAGGGGCCAGCAGCCCCTCGGGACCTGGCCACCTTTGCCAGCACCAGC ACTCTGCATGGGCTGGGCCGGGCCTGTGGCCCTGGCCCCCATGGACTGCGCAGAACCCTGTGGGCACTGG CCCTACTCACCTCACTGGCTGCCTTTCTGTACCAGGCAGCCAGCCTGGCCAGGGGCTACCTGACCCGGCC TCACCTGGTAGCCATGGACCCTGCTGCCCCAGCCCCAGTGGCGGGTTTTCCGGCTGTCACCCTCTGCAAC ATCAACCGCTTCCGGCACTCGGCACTCAGCGATGCTGATATCTTCCACCTGGCCAATCTGACAGGGCTGC CCCCCAAAGACCGGGACGGGCACCGTGCAGCTGGCCTTCGCTATCCAGAGCCTGACATGGTAGACATCCT CAACCGCACTGGCCACCAGCTTGCTGACATGCTCAAGAGCTGCAACTTCAGTGGGCACCACTGCTCCGCC AGCAACTTCTCTGTGGTCTATACTCGCTATGGGAAGTGTTACACCTTTAATGCAGATCCTCAGAGTTCAC TGCCCAGTAGGGCAGGCGGCATGGGTAGTGGCCTGGAGATCATGCTAGACATCCAGCAGGAGGAATACCT ACCCATATGGAGGGAGACAAACGAGACGTCATTCGAGGCAGGGATCCGGGTGCAGATCCACAGCCAGGAG GAGCCGCCCTACATCCACCAGCTGGGGTTTGGCGTGTCCCCAGGCTTCCAGACTTTTGTGTCCTGCCAGG AACAGCGGCTAACTTATCTGCCCCAGCCTTGGGGCAACTGCCGGGCGGAAAGCGAGCTCAGGGAGCCTGA GCTTCAGGGCTACTCAGCCTATAGTGTGTCTGCCTGCCGACTGCGCTGTGAGAAGGAGGCCGTGCTTCAG CGCTGCCACTGCCGGATGGTGCACATGCCAGGCAACGAGACCATCTGCCCACCAAATATCTACATTGAAT GTGCCGACCACACACTGGACTCCCTTGGTGGGGGCTCCGAGGGCCCATGCTTCTGCCCTACACCCTGCAA CCTGACTCGTTACGGCAAAGAGATCTCCATGGTCAAGATCCCCAACAGGGGCTCAGCCAGATACCTGGCA AGGAAGTACAACCGCAATGAGACCTATATAAGGGAGAACTTCTTGGTCCTGGATGTCTTTTTCGAAGCCC TAACTTCTGAAGCCATGGAACAGCAAGCCGCCTATGGCCTGTCAGCCCTGCTGGGGGACCTTGGGGGACA GATGGGCCTGTTCATTGGGGCTAGCATCCTCACCTTGCTGGAGATCCTTGACTACATCTATGAGGTCTCC TGGGATCGGCTCAAGAGGGTGTGGCGACGGCCCAAGACCCCACTGCGGACATCCACCGGGGGCATCTCCA CTCTGGGGCTGCAGGAGCTGAAGGAACAGAGTCCCTGTCCAAGCCGGGGCCGTGCTGAGGGTGGGGGGGC TAGCAGCCTGCTTCCCAACCATCACCACCCTCACGGCCCCCCAGGAAGCCTCTTTGAAGACTTTGCTTGC TAGAACAGTGCTGTGTGGGGAAAGTACCCATGAAACTGGGCCTCTACGCTGACCCCACACATCTCCTATT CCTGGGACAGAAGGTCTGGGGCAGCCCAGGGCTAAGGGAAGGGGTGGTGCTCACTGAGAGGCCAGGACTA GGGTCCTGCTCTCCCTGACCCAGGCTCAGCTGCCTTGCACAAGGGTCCTTCTTGTCTATACTCCCTGCTC CCAGGCAGGTATCCAGGAAGGGCTGGAGACCAGACTAGAGGTCCCTGAGGAGGGGAGGGAGGAAGAGGGG GGGAAGGTGGAACCCTGGCAGAGCCCCTCTGTACATTTGTATATAGTTAGGGACTGGGTGGGGGTGGGAC ACAGACATAGAAGGGTGGGACTGCAGGGGAGGGTGACACAGGACAGTCAGGGTCCTAGCCCTAATGGCAG AAGGCAAGTCCTTGGGACCCAGGCATGCTGGGCTGGTTCTCTTTCCCTCTTTCCAGGCCCAGCTCCCTCT TGGCAGGGGGAGTGGGCGGCCCAGCAGGCCTGGCCCAGCTCCCAGTTCCCCCTGTACCAGCCCCACCACA AGTTCCCTTTATGGGGAGTGGGTGGAAAGACCTTTCAGACCTTGGCTGAGCATAGGGGGAGGGGGAGGGG GAGCGGCCTTCTCAGGCCTTGCTCCCTAGAGATTGGTTTTATAAAGTGCTGGTGAAATTGGGAATAAAGA GGCACAAAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808924 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808924 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7691 same embryo
	EMAGE:7693 same embryo
	EMAGE:7688 same embryo
	EMAGE:7690 same embryo
	EMAGE:7689 same embryo
	EurExpress:euxassay_005297 same experiment
	MGI:4822914 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source