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EMAGE:7700

Fkbp9 FK506 binding protein 9 ( MGI:1350921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700
"Pseudo-wholemount" of euxassay_007465. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007465_01 euxassay_007465_02 euxassay_007465_03 euxassay_007465_04
EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700
euxassay_007465_05 euxassay_007465_06 euxassay_007465_07 euxassay_007465_08 euxassay_007465_09
EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700
euxassay_007465_10 euxassay_007465_11 euxassay_007465_12 euxassay_007465_13 euxassay_007465_14
EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700
euxassay_007465_15 euxassay_007465_16 euxassay_007465_17 euxassay_007465_18 euxassay_007465_19
EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700 EMAGE:7700
euxassay_007465_20 euxassay_007465_21 euxassay_007465_22 euxassay_007465_23 euxassay_007465_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7700Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7700_wholemount_strong.wlz
7700_wholemount_moderate.wlz
7700_wholemount_weak.wlz
7700_wholemount_possible.wlz
7700_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7700_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 19 20 21 22 23 moderate expression: see section 01 05 06 07 15 16 17 24
radius
moderate moderate
regionalmoderate expression: see section 01
ulna
moderate moderate
regionalmoderate expression: see section 01
humerus
moderate moderate
regionalmoderate expression: see section 02 03 04 05 22 23 24
hindlimb digit 1 metatarsal
moderate moderate
regionalmoderate expression: see section 23 24
hindlimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 06 23
hindlimb digit 2 metatarsal
moderate moderate
regionalmoderate expression: see section 23 24
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 06 07 08 22 23
hindlimb digit 3 metatarsal
moderate moderate
regionalmoderate expression: see section 23
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 06 07 09 10 22 23
hindlimb digit 4 metatarsal
moderate moderate
regionalmoderate expression: see section 23
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 06 07 09 21 22 23
hindlimb digit 5 phalanx
moderate moderate
regionalmoderate expression: see section 09 21 22
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 02 05
fibula
moderate moderate
regionalmoderate expression: see section 01 02 03
tibia
moderate moderate
regionalmoderate expression: see section 01 02 03 04 24
femur
moderate moderate
regionalmoderate expression: see section 03 04 24
otic capsule
strong strong
regionalstrong expression: see section 16 17 18 19 20
nasal septum
strong strong
regionalstrong expression: see section 10 11 13 14 moderate expression: see section 12
mandible
moderate moderate
regionalmoderate expression: see section 10 11 20 21 22 weak expression: see section 03 04 05 06 07 08 09 15 16 17 18 19
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 01 13
maxilla
moderate moderate
regionalmoderate expression: see section 20 weak expression: see section 05 06 07 08 09 17 18 19
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 13
bladder
moderate moderate
regionalmoderate expression: see section 11 12 13 14
cricoid cartilage
moderate moderate
regionalmoderate expression: see section 12
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
axial skeleton
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
nucleus pulposus
strong strong
regionalstrong expression: see section 13
exoccipital bone
strong strong
regionalstrong expression: see section 03 04 05 06 07 20 21 22 23
temporal bone
strong strong
regionalstrong expression: see section 04 05 06 07 21 24
vault of skull
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 20 21 22 23 24
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 13 14 17 18 19 20 21 22 23 24
viscerocranium
strong strong
regionalExpression in the turbinate bone.
clavicle
moderate moderate
regionalmoderate expression: see section 08 09 16 17 weak expression: see section 10
sternum
moderate moderate
regionalmoderate expression: see section 13 14
tail mesenchyme
moderate moderate
regionalmoderate expression: see section 12 13 16 17
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8968
Entity Detected:Fkbp9, FK506 binding protein 9 ( MGI:1350921)
Sequence:sense strand is shown

>T8968
GGCTCCTCCCCTCGGCCCGGCACGGGGCGGACGCGGGCGGCGGGGGGCGGGCGCTGCGGCCCGGGGCTAG
CCGGGAGACCTTTCCACGTTTGCATCGCCGCGGCGCGCCCAGCCTTGCCCCTCGCCTCCCCGGAGTCCAG
GCCGCGCGATGGCATTAGGGGCCCGGGGCTGGCGGCGCCGGTCGCTGCTCCTGCTGCTGCTCTGGGTGAC
CGGGCAGGCAGCCCCGGTGTTGGGCCTGGCTGTGAGCTCGGAACTTCAGATCCAGCAGAGCTTCGTGCCT
GATGAGTGTCCGCGCACGGTGCACAGTGGCGACTTCGTGCGCTACCACTACGTGGGGACTTTCCTCGACG
GCCAGAAGTTCGACTCCAGCTATGACAGGGACTCTACCTTCAATGTGTTTGTGGGAAAAGGCCAGCTGAT
CGCAGGGATGGACCAAGCTCTTGTTGGGATGTGCGTAAACGAGAGGCGTCTTGTGACGATTCCCCCAAAC
CTTGCCTACGGAAGTGAAGGAGTTTCTGGTGTGATCCCTCCAAACTCAGTTCTTCATTTTGATGTACTCT
TGGTGGATATCTGGAATTCTGAAGACCAGGTTCACATTCAGACCTATTTCAAGCCTCCCAGTTGCCCTCG
GACTATCCAAGTATCGGATTTTGTGAGGTACCACTACAACGGAACGTTCTTGGATGGGACACTGTTTGAT
TCCAGCCATAATCGCATGAAAACCTATGACACGTACGTGGGGATTGGATGGCTGATCCCTGGAATGGACA
AAGGGCTGCTGGGCATGTGTGTCGGCGAGAAGCGTATCATCACAGTCCCCCCTTTTCTGGCCTACGGGGA
GGAGGGAGATGGAAAAGACATCCCTGGGCAGGCATCCTTAGTCTTTGATGTTGCATTGTTGGACCTCCAT
AACCCCAAGGATACCATTTCCATTGAGAATAAGGTGGTTCCTGAGAACTGTGAGAGGAGAAGTCAGAGTG
GGGACTTCCTCAGGTATCATTACAATGGCACACTTCTGGACGGCACTCTCTTTGACTCCAGCTATTCACG
GAACCACACCTTTGACACATACATTGGGCAGGGCTATGTGATTCCTGGGATGGATGAAGGCTTACTTGGT
GTCTGCATTGGGGAGAGGCGAAGGATTGTGGTCCCTCCTCACCTGGGCTACGGAGAAAAAGGCAGAGGGA
GCATTCCTGGCTCAGCCGTGCTGGTGTTCGACATCCACGTGATTGACTTTCACAACCCTTCGGATTCCAT
CAGCATCACTTCCCACTACAAGCCTCCAGACTGCTCGGTGCTGAGTAAGAAGGGGGATTACCTCAAATAC
CACTACAATGCCTCACTCCTGGATGGGACTCTGCTGGACTCCACGTGGAACTTAGGCAAAACGTATAACA
TTGTGTTGGGGTCTGGACAAGTCGTGCTGGGGATGGACATGGGTCTCAGAGAGATGTGCGTGGGAGAGAA
GCGGACTGTGATCATCCCGCCCCACCTGGGCTATGGAGAAGCTGGTGTGGATGGAGAGGTCCCTGGCAGT
GCCGTCCTAGTGTTTGACATCGAACTGCTGGAGCTTGTGTCTGGCCTTCCGGAGGGGTACATGTTCATAT
GGAATGGTGAAGTGTCTCCCAACCTCTTTGAAGAAATCGACAGGGATGGCAATGGGGAAGTCCTCCTGGA
GGAGTTCTCAGAGTACATTCATGCCCAAGTGGCAACAGGCAAAGGGAAGCTGGCTCCTGGCTTTAATGCT
GAAATGATCGTGAAGAATATGTTCACCAACCAGGACCGGAGTGGAGATGGGAAGGTCACGGCTGAGGAAT
TTAAACTGAAGGATCAGGAAGCCAAACATGATGAACTGTAAGCCTGACACAGATCAGTGTGTGCCAAGGA
GTGCATGACACCAAGCGACCTGTCGTGGCGGAGCATGCTGTCAAGGTGTGAAGCTTTCTAGGAAGGTTTG
AGAGTAGCCAGAATTAGGTGGGCCATGTAGTACCTGGTGCAGACAGTGGGGGGTGGCTGATGCCCACGGT
GAGGACTTGGGTGGATTTCCAGTGACAGTAAAGAGAATCTGTATATAGTGCTTTGGCACAAATGCAATTG
GTCTGAAGCACCAATTGCACTTGTGAGTCTTCTGGGAGGTTGTGTTAATAAAGGAAAATTATTTCATAGA
AGATGTAGCTGTCATGGAGGAAACAGAAGAAGTACCCAGGTGTCTACCTAAAGAAACGATTTGGTTTAAA
AGTCTTGCTAGTGTCTTCAAAAAAGTTCACCATGGGCAGAAAGATAAAGGCACACATGTTTCAGCAAGTG
TCCTTGACAGCAGCCATTGCTCTTCTCAGTGTTCTGTGCAAGCTCTGGTGATACCAGGCCCCGTGCGACA
GCCATCCTGTCACCTTCCCTACAAATCTCCATTTTCATGCTTTCCAGTTCACTCTCTACACTGGCAGCCC
TCCAATTGGTAGTGTCTGTCACTTAAAGGTTAACAGGAGTCTCCTGGACAGGAAAAACACAGACCAAATG
GAAGCTGTCTTTAGTCCATGAGTAGAGGATTTGACCTGCATTTTCTACATAGGATTGGCTTTCTCTTTCT
CCACTCTATTCCTTTGGGGAGATACATGATGGCTGGGTCTCACTGGTCGGCCAACAGAAAATTCTACTGT
GACTAAAGTGGATGTGTTATCCCAACAAATGAAGGTGACACTTTCTGTTCTCCCCCCCCCCATTTGTGTC
TTGGAAGGCAATTTGAGGTCTCCTGCTGTAGAAAAGGCTAGCCCATTCCCACACCATGTGGTAGCTATGC
TCCTACTTTATGTCCTCAGATACCAATGATGTTGGCACTCACTCTGACGTAATCAGGACATTTCCGTTTG
AAATGGCGTTTTTTACAAAGTTAAACTGTTTTGTACTGATGTTGCCCTGAGATAGTTCATGCAAATGCTG
TGCACTCATTCAGTGGAATATAGTTGGAAAACTGACTTCTTTTTTTCTGACATAAAATGTTGAATCATAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6810029 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810029 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7704 same embryo
 EMAGE:7703 same embryo
 EMAGE:7702 same embryo
 EMAGE:7705 same embryo
 EMAGE:7701 same embryo
 EurExpress:euxassay_007465 same experiment
 MGI:4824875 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS