EMAGE mouse gene expression spatial database

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EMAGE:7742

Nuak2 NUAK family, SNF1-like kinase, 2 ( MGI:1921387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007500. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007500_01	euxassay_007500_02	euxassay_007500_03	euxassay_007500_04

euxassay_007500_05	euxassay_007500_06	euxassay_007500_07	euxassay_007500_08	euxassay_007500_09

euxassay_007500_10	euxassay_007500_11	euxassay_007500_12	euxassay_007500_13	euxassay_007500_14

euxassay_007500_15	euxassay_007500_16	euxassay_007500_17	euxassay_007500_18	euxassay_007500_19

euxassay_007500_20	euxassay_007500_21	euxassay_007500_22	euxassay_007500_23	euxassay_007500_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7742_wholemount_strong.wlz

7742_wholemount_moderate.wlz

7742_wholemount_weak.wlz

7742_wholemount_possible.wlz

7742_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7742_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15

telencephalon ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

pons ventricular layer

strong

regional

strong expression: see section 12 13

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16

cervical ganglion

strong

regional

strong expression: see section 09 16 17

thoracic ganglion

strong

regional

strong expression: see section 11 moderate expression: see section 12

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

anterior naris

moderate

regional

moderate expression: see section 12 13 14 15

external naris

moderate

regional

moderate expression: see section 10

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 14 15 16 17 18 weak expression: see section 07

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13

right lung

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9056
Entity Detected:	Nuak2, NUAK family, SNF1-like kinase, 2 ( MGI:1921387)
Sequence:	sense strand is shown >T9056 GCGGCTGTGACCTCTGAGCCCGCGGCTCAGCGCGCGCTGCTACTGCTGCCCGACCCACTCCACCTCGCGG TCCCCGCACCATGGAGTCGGTGGCCTTACTCCAGCGCCCGAGCCAGGCTCCCTCGGCCTCCGCCCTGGCC TCGGAGAGCGCCCGGCCGCTGGCGGACGGGCTCATCAAGTCGCCTAAACCTCTGATGAAGAAGCAGGCGG TGAAGCGGCACCATCACAAACACAACCTGCGGCACCGCTACGAGTTCCTGGAGACGCTGGGCAAGGGCAC CTACGGGAAGGTGAAGAAGGCACGAGAGAGCTCGGGGCGTCTGGTGGCCATCAAGTCCATCAGGAAAGAC AAAATCAAAGATGAGCAGGATCTGCTGCACATACGGAGGGAGATTGAGATCATGTCTTCACTCAACCACC CCCACATCATTGCCATCCATGAAGTGGGCAGATCACGCTTAGTGACTGTGTTTGAGAATAGCAGCAAGAT TGTGATTGTCATGGAGTATGCCAGCCGAGGCGATCTGTATGATTACATCAGTGAGCGGCCACGGCTGAGT GAGCGGGACGCCAGGCATTTCTTCCGACAGATCGTGTCTGCCCTGCACTACTGCCACCAGAACGGGATCG TTCACCGAGATCTCAAGCTGGAAAACATCCTTCTAGATGCCAATGGAAACATCAAGATTGCTGACTTTGG CCTCTCCAACCTGTACCACAAAGGCAAGTTCCTCCAGACGTTCTGTGGGAGCCCTCTCTACGCCTCGCCT GAGATAGTCAACGGGAAGCCCTATGTGGGCCCAGAGGTGGACAGCTGGTCTCTGGGCGTTCTCCTGTACA TCCTGGTGCATGGCACCATGCCCTTTGACGGGCAGGATCATAAAACACTGGTGAAGCAAATCAGTAACGG GGCTTACCGTGAGCCGCCCAAGCCGTCCGATGCCTGTGGCCTGATCCGGTGGCTGTTAATGGTGAACCCC ACCCGTCGGGCCACACTGGAGGATGTAGCCAGTCATTGGTGGGTCAACTGGGGTTACACCACCGGAGTCG GGGAACAGGAAGCCCTGCGTGAGGGTGGGCACCCTAGTGGTGACTTTGGCCGGGCCTCCATGGCGGACTG GTTACGTCGCTCCTCGCGCCCCCTCCTGGAGAATGGAGCCAAGGTGTGCAGCTTCTTCAAGCAGCACGTG CCGGGAGGTGGAAGCACTGTACCTGGGCTGGAGCGGCAACATTCTCTTAAGAAGTCCCGAAAGGAGAATG ACATGGCTCAAAATCTGCAAGGTGACCCGGCTGAGGATACCTCTTCTCGCCCTGGCAAGAGCAGCCTTAA GCTTCCGAAAGGCATTCTCAAGAAAAAGTCCTCTACCTCGTCAGGGGAGGTACAGGAGGACCCTCAGGAA CTCAGACCGGTGCCTGATACTCCAGGGCAGCCTGTCCCTGCTGTATCCCTGCTCCCAAGGAAAGGCATCC TTAAGAAGTCTCGACAGCGTGAATCTGGTTACTACTCCTCTCCAGAGCCCAGCGAGTCTGGGGAACTCTT AGACGCCAGTGATGTGTTTGTGAGTGGGGACCCCGTGGAGCAGAAGTCTCCACAGGCTTCAGGGCTCCTC CTCCACCGCAAGGGCATTCTCAAACTCAATGGCAAGTTCTCCCGCACAGCCTTAGAAGGCACTACCCCTA GCACCTTTGGCTCCCTGGACCAACTGGCCTCCTCCCATCCTGCAGCCCGGCCCAGCCGCCCCTCAGGGGC TGTGAGTGAGGACAGCATCCTGTCCTCCGAGTCCTTTGACCAATTGGACTTGCCTGAACGTCTTCCCGAA ACCCCACTGAGGGGCTGTGTGTCTGTGGACAACCTGAGGGGGCTTGAGCAGCCTCCCTCAGAAGGTCTGA AGCGATGGTGGCAGGAATCCTTGGGGGATAGCTGCTTTTCTCTGACAGACTGCCAAGAGGTGACTGCAGC CTACAGACAAGCCCTAGGAATCTGCTCAAAGCTCAGCTGAGGAAGGGAGATGGTGCCCTAGTATGGGGTA GGCTCTGAGAGGGTTTGCAGAGGAACCCTGGGTCGGATTCCTCCAGTGAATAGAGTACATCAAGGGCTCT ACGTCTGCAGCCTGACTGAACCTGAAAGATGAGAGAAATCGCATTGATGTGGAAAGGAATGGGAACCCTT GCTGCCCGAGTGTTATAGTGGGGTGGCCTGAAGGTGCCTACCTCCTTTGTGCCATGAGTGTCACCCATGA CATTTCCCACCCTGTTCTCTGGCTGCACCTTCACATAAGTTTCTGTTTCCATCAACCACCAGGGTTAGAA CCCTGACTTCCTGGGAGGTAATGTGTAGTGACTGCCATTATTTAGAGAGGAAACAGCCTCTGGTTTCCAT CTCTGCTGCTGTGCATCTCAAAGACCTGGGAAGACTCGGACCGCTGTTTGACTTCATCTCAAGGGGACCA GATGCCCCTGGACCCCATCTTAGATCTCAGAGACTTGAACCTTGAAGCTGTTCCTAGTACCCAGATGTGG ATGGATGCTCTGTTTCTCAGGCCAACGGGACCTAGAATGTGCTGACTTATTTATTTTTTTGTGATTCTCA CTTCTGTTTTTTGGTTTTTGTTTGTTTGTTTGTTTTTGTTTTTAAGTGAATTTTGCTGCTTTCAATAATG TGAATGCTGTGTTCTGGGGAACTCCACTGTGCCACTGAAGTTTATGTACAGAGAAGTATTTGGCAATGAT GTCCCTCTATTCAAGGGGGGTGGGGGCGTTTTTCAAATGTATGTCTTGAGCACTGTCTGGATTGAGTCTC CAGTCCCTTCACACCCAAGGCTGGCCACCCTCCCTCATCTTCATCTGTGGCCAAAAAAAAAAAAAAAAAA AAAAAAACAACCCTCATTAAAACCAGCAGTGGTAACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816569 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816569 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7744 same embryo
	EMAGE:7746 same embryo
	EMAGE:7741 same embryo
	EMAGE:7743 same embryo
	EMAGE:7745 same embryo
	EurExpress:euxassay_007500 same experiment
	MGI:4826824 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source