EMAGE mouse gene expression spatial database

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EMAGE:7743

Atxn7 ataxin 7 ( MGI:2179277)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007505. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007505_01	euxassay_007505_02	euxassay_007505_03	euxassay_007505_04

euxassay_007505_05	euxassay_007505_06	euxassay_007505_07	euxassay_007505_08	euxassay_007505_09

euxassay_007505_10	euxassay_007505_11	euxassay_007505_12	euxassay_007505_13	euxassay_007505_14

euxassay_007505_15	euxassay_007505_16	euxassay_007505_17	euxassay_007505_18	euxassay_007505_19

euxassay_007505_20	euxassay_007505_21	euxassay_007505_22	euxassay_007505_23	euxassay_007505_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7743_wholemount_strong.wlz

7743_wholemount_moderate.wlz

7743_wholemount_weak.wlz

7743_wholemount_possible.wlz

7743_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7743_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

mesenchyme

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

strong

regional

strong expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 13 14 15 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 12 13

central nervous system ganglion

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

central nervous system nerve

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

peripheral nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3332
Entity Detected:	Atxn7, ataxin 7 ( MGI:2179277)
Sequence:	sense strand is shown >T3332 TGGCCTCGAGCCAGATTCGGACGAGGTCTGAAGTGGGAGGAACAGCCAAGGCAGGCTTGCTAGATGAGCT ATGTCTTCTTTCATAGTGAGATTGAAATGCCTGCGGTTTGACAACTTGGTTGCAGTCATTACACACCACC AAGTAGAAATCATCATGTGCTGGGCAGAGACCAAAGATCGGCATATCTTCCCGGCAGAGCCCCATGACTT CGCGGTTTTTCCCAAACTCCTTGAAACTTTCATCCAATTCGGTCCCATCCTTCCCAGGAAGTTTGGAAGC TTCCACCCACAGATTCCACGACTGTCCCAGCATCGCTTCGGGACTGGGCAAGGGCCTGCGCTCCCCAACC GTCGCCATGGCGGCGGCCGAGGTGGTGGTGTCCCCGGTGCCGCCGTCCTCCGCCCGTGGGCGCCGCAGCG GCGGGTGCTGCCGCTGGGGCTGCAGAGGCTGTGGCTGCTGCTGCTGCTGCCGG
Notes:	The probe template was PCR amplified from IMAGE:314478 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:314478 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7744 same embryo
	EMAGE:7746 same embryo
	EMAGE:7742 same embryo
	EMAGE:7741 same embryo
	EMAGE:7745 same embryo
	EurExpress:euxassay_007505 same experiment
	MGI:4823350 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source