EMAGE mouse gene expression spatial database

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EMAGE:7748

1190002H23Rik RIKEN cDNA 1190002H23 gene ( MGI:1913464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007417. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007417_01	euxassay_007417_02	euxassay_007417_03	euxassay_007417_04

euxassay_007417_05	euxassay_007417_06	euxassay_007417_07	euxassay_007417_08	euxassay_007417_09

euxassay_007417_10	euxassay_007417_11	euxassay_007417_12	euxassay_007417_13	euxassay_007417_14

euxassay_007417_15	euxassay_007417_16	euxassay_007417_17	euxassay_007417_18	euxassay_007417_19

euxassay_007417_20	euxassay_007417_21	euxassay_007417_22	euxassay_007417_23	euxassay_007417_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7748_wholemount_strong.wlz

7748_wholemount_moderate.wlz

7748_wholemount_weak.wlz

7748_wholemount_possible.wlz

7748_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7748_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between hindlimb digits 1 and 2 mesenchyme

strong

regional

strong expression: see section 03 04

interdigital region between hindlimb digits 2 and 3 mesenchyme

strong

regional

strong expression: see section 02 03 21 22

interdigital region between hindlimb digits 3 and 4 mesenchyme

strong

regional

strong expression: see section 03 06 21 22

interdigital region between hindlimb digits 4 and 5 mesenchyme

strong

regional

strong expression: see section 03 06 21 22

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16 weak expression: see section 11

thyroid gland

moderate

regional

moderate expression: see section 10 11 12 15 16

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14 15

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 15 16

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 16 17 18 19 20 21 22 moderate expression: see section 15

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 16 17 23 24 moderate expression: see section 12 13 15

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16 17

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14 weak expression: see section 15 16

pons mantle layer

weak

regional

weak expression: see section 08 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 moderate expression: see section 04 07 08 09 17 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 17 weak expression: see section 12 13 14 15 16

spinal cord ventricular layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

dorsal root ganglion

moderate

regional

moderate expression: see section 12 13 17 18 weak expression: see section 08 10 11 19

mandible

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 weak expression: see section 04 05 06 15 16 17 18 19 20 21 22

maxilla

weak

regional

weak expression: see section 05 06 20

liver

weak

regional

weak expression: see section 01

liver left lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

liver right lobe

weak

regional

weak expression: see section 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9073
Entity Detected:	1190002H23Rik, RIKEN cDNA 1190002H23 gene ( MGI:1913464)
Sequence:	sense strand is shown >T9073 GTAGCTAGCGCTCAGGCTCGGCAAGGAGCAAGGCAGAGGCACCGGAGCAGACTGCAGTGCAGGCGGCAGC CACCAGGGCCAACACCTCGCAGCATCTCTGCTACGCGTGTCACTGAGCAACTTCTCCATGAAGCCGCCCT CAGCGCAGAGCAGTCCCGCGGCGGTCGCGGCCGCAGCCCCGGCCATGGACTCCGCGGCCGCTGCAGACCT GACCGACGTGCTGTGCGAGTTCGACGCGGTGCTGGCCGACTTCGCGTCGCCCTTCCACGAGCGCCACTTC CACTATGAGGAGCACCTAGAGCGCATGAAGCGGCGCAGCAGCGCCAGCGTCAGCGACAGCAGCGGCTTCA GCGACTCGGAGAGTGCAGACTCAGTGTACAGGGACAGCTTCACCTTCAGTGATGAGAAGCTGAATTCTCC AACCAACTCCTCTCCAGCTCTCCTGCCCTCCGCTGTCACTCCTCGGAAAGCCAAATTAGGTGACACTAAA GAGCTCGAAGACTTCATTGCCGATCTGGACAGGACCTTAGCAAGTATGTGAAGCAAGGAGTTTGGGGTCC AGAAGGCTCCGAGGACCTGGCAAATCGGCTACTAGAATCTGCTGTGGGAGAGGGCAGAGCTGAGGCTCCT GCCCCCTGGCCATTCTTGGTTCACTATAACATTAGCCATTGGGCCCATCTCTGGGCAGTTCGGAGAGTGA AGCTCACTTTGTTTACCTACCTGCAGCATATTCAACAGAGGATCTATCTAATGTGTTTTTACTCCTTTAA ACATAGCTCTTCTATAATTTAAAATGCTTTTATGGAAATATTTGTAATTACTTATATATAGTTGGAGGTC ATAATAAGCTTTCCCATCATAATATATTTTTGTATGCAAATAAAATTAAAACGGAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6818687 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6818687 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7750 same embryo
	EMAGE:7747 same embryo
	EMAGE:7749 same embryo
	EurExpress:euxassay_007417 same experiment
	MGI:4822601 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source