EMAGE mouse gene expression spatial database

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EMAGE:7877

Brd2 bromodomain containing 2 ( MGI:99495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012809. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012809_01	euxassay_012809_02	euxassay_012809_03	euxassay_012809_04

euxassay_012809_05	euxassay_012809_06	euxassay_012809_07	euxassay_012809_08	euxassay_012809_09

euxassay_012809_10	euxassay_012809_11	euxassay_012809_12	euxassay_012809_13	euxassay_012809_14

euxassay_012809_15	euxassay_012809_16	euxassay_012809_17	euxassay_012809_18	euxassay_012809_19

euxassay_012809_20	euxassay_012809_21	euxassay_012809_22	euxassay_012809_23	euxassay_012809_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7877_wholemount_strong.wlz

7877_wholemount_moderate.wlz

7877_wholemount_weak.wlz

7877_wholemount_possible.wlz

7877_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7877_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 16 17 18

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15

facial vii ganglion

weak

regional

weak expression: see section 04 05 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 07 08 16

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 14 15 16

mandible

weak

regional

weak expression: see section 05 06 07 08 09 14 15 16 17 18

lower jaw incisor

weak

regional

weak expression: see section 09 10 13 14

maxilla

weak

regional

weak expression: see section 08 09 16 17

upper jaw incisor

weak

regional

weak expression: see section 10 13 14 15

orbito-sphenoid

weak

regional

weak expression: see section 02 03 04 05 06 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38181
Entity Detected:	Brd2, bromodomain containing 2 ( MGI:99495)
Sequence:	sense strand is shown >T38181 TAGTGATGAAGGCTCTGTGGAAGCATCAGTTTGCATGGCCATTCCGGCAGCCTGTGGACGCTGTGAAGCT GGGTTTGCCGGATTATCACAAAATTATAAAACAGCCTATGGACATGGGTACTATCAAGAGGAGACTTGAA AACAATTACTACTGGGCTGCCTCAGAATGTATGCAGGATTTTAATACTATGTTTACCAACTGTTACATTT ATAACAAGCCCACCGATGATATTGTCCTAATGGCACAGACACTGGAAAAGATCTTCCTACAGAAAGTGGC ATCCATGCCACAAGAGGAGCAAGAGCTTGTGGTGACCATCCCTAAAAACAGCCATAAGAAGGGGGGCAAG TTAGCAGCACTCCAGGGCAGTATTACCAGTGCCCATCAGGTGCCTGCTGTCTCTTCTGTGTCGCATACAG CCCTGTATACACCACCACCTGAAATACCTACCACTGTCCTCAACATTCCCCACCCATCAGTCATCTCTTC TCCTCTTCTTAAGTCCCTGCATTCTGCTGGACCCCCACTCCTTGCTGTATCAGCAGCGCCTCCAGCTCAG CCCCTTGCCAAGAAAAAAGGCGTTAAACGGAAAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104182. Forward Primer - name:104182_F_cDNA_Brd2, sequence:TAGTGATGAAGGCTCTGTGGAA; Reverse Primer - name:104182_N_SP6_cDNA_Brd2, sequence:GCTTTCCGTTTAACGCCTTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7881 same embryo
	EMAGE:7879 same embryo
	EMAGE:7880 same embryo
	EMAGE:7876 same embryo
	EMAGE:7878 same embryo
	EurExpress:euxassay_012809 same experiment
	MGI:4823501 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source