EMAGE mouse gene expression spatial database

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EMAGE:8308

Npnt nephronectin ( MGI:2148811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007623. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007623_01	euxassay_007623_02	euxassay_007623_03	euxassay_007623_04

euxassay_007623_05	euxassay_007623_06	euxassay_007623_07	euxassay_007623_08	euxassay_007623_09

euxassay_007623_10	euxassay_007623_11	euxassay_007623_12	euxassay_007623_13	euxassay_007623_14

euxassay_007623_15	euxassay_007623_16	euxassay_007623_17	euxassay_007623_18	euxassay_007623_19

euxassay_007623_20	euxassay_007623_21	euxassay_007623_22	euxassay_007623_23	euxassay_007623_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8308_wholemount_strong.wlz

8308_wholemount_moderate.wlz

8308_wholemount_weak.wlz

8308_wholemount_possible.wlz

8308_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8308_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 02 03 04 21 22

upper leg muscle

moderate

regional

moderate expression: see section 01 02 03 04 20 21 22 23

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 20 21 22 23 24 weak expression: see section 10 11 13 14 15 16 17 18 19

pericardial cavity

moderate

regional

moderate expression: see section 19 20 weak expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18

pleural cavity

moderate

regional

moderate expression: see section 19 20 weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 07 08 09 19 20 21

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 19 20 21 22 23 24 moderate expression: see section 07 08 09 10 11 12 13 16 17 18

vibrissa

weak

regional

weak expression: see section 06 07 08 21

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 14 moderate expression: see section 10 11 15

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 15 moderate expression: see section 08 09 10 11 13

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 16 17 18 moderate expression: see section 09 10 13 14 15 weak expression: see section 11 12

ventral grey horn

strong

single cell

strong expression: see section 10 moderate expression: see section 11 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 15 16 17 18 weak expression: see section 08

nasal septum

strong

regional

strong expression: see section 09 10 17 18 19 moderate expression: see section 11 12 13 15 16

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 weak expression: see section 06

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

mandible

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 moderate expression: see section 03 04

lower jaw incisor

strong

regional

strong expression: see section 11 12 13 15 16

maxilla

strong

regional

strong expression: see section 06 07 08 09 10 11 12 15 16 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

metanephros

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19

genital tubercle of male

moderate

regional

moderate expression: see section 10 11 12 13

vault of skull

strong

regional

strong expression: see section 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 20 21 22 23 24

clavicle

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36697
Entity Detected:	Npnt, nephronectin ( MGI:2148811)
Sequence:	sense strand is shown >T36697 AACACGTTTGGGAGCTACATCTGCAAGTGTCACACTGGTTTCGACCTCATGTACATTGGAGGCAAATATC AGTGCCATGACATCGACGAGTGCTCTCTTGGACAGCACCAGTGTAGCAGCTATGCCCGGTGTTACAACAT ACATGGGTCCTACAAGTGCCAATGTAGAGATGGATACGAGGGGGATGGACTGAACTGTGTGTATATCCCC AAAGTCATGATTGAACCTTCAGGTCCAATCCATATGCCAGAAAGAAATGGTACAATCTCAAAGGGTGATG GAGGACATGCGAATAGGATTCCTGATGCTGGAAGTACAAGGTGGCCCCTGAAGACACCATATATTCCTCC TGTCATTACCAACAGGCCTACTTCCAAGCCAACAACAAGACCTACACCAAACCCAACACCACAGCCTACT CCACCACCTCCACCACCCCTCCCGACAGAGCCCAGAACAACTCCACTACCACCAACCCCAGAAAGGCCAT CTACCAGACCCACCACTATAGCACCTGCTACCAGTACCACTACACGAGTAATTACGGTTGACAACAGGAT ACAGACGGATCCTCAGAAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91989. Forward Primer - name:091989_F_cDNA_Npnt, sequence:AACACGTTTGGGAGCTACATCT; Reverse Primer - name:091989_N_SP6_cDNA_Npnt, sequence:TTTCTGAGGATCCGTCTGTATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8309 same embryo
	EMAGE:8311 same embryo
	EMAGE:8307 same embryo
	EMAGE:8312 same embryo
	EMAGE:8310 same embryo
	EurExpress:euxassay_007623 same experiment
	MGI:4826766 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source