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EMAGE:8355

Phospho1 phosphatase, orphan 1 ( MGI:2447348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355
"Pseudo-wholemount" of euxassay_007708. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007708_01 euxassay_007708_02 euxassay_007708_03 euxassay_007708_04
EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355
euxassay_007708_05 euxassay_007708_06 euxassay_007708_07 euxassay_007708_08 euxassay_007708_09
EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355
euxassay_007708_10 euxassay_007708_11 euxassay_007708_12 euxassay_007708_13 euxassay_007708_14
EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355
euxassay_007708_15 euxassay_007708_16 euxassay_007708_17 euxassay_007708_18 euxassay_007708_19
EMAGE:8355 EMAGE:8355 EMAGE:8355 EMAGE:8355
euxassay_007708_20 euxassay_007708_21 euxassay_007708_22 euxassay_007708_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8355Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8355_wholemount_strong.wlz
8355_wholemount_moderate.wlz
8355_wholemount_weak.wlz
8355_wholemount_possible.wlz
8355_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8355_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 22 23
radius
strong strong
regionalstrong expression: see section 22 23
ulna
strong strong
regionalstrong expression: see section 23
humerus
strong strong
regionalstrong expression: see section 20 21 22 23
fibula
strong strong
regionalstrong expression: see section 01 23
tibia
strong strong
regionalstrong expression: see section 01 02 23
femur
strong strong
regionalstrong expression: see section 03 04 05 06 21 22 23
mandible
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 10 11 12 13 14 15 16 17 18 19 20 22 23
maxilla
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 12 13 14 15 16 17 18 19 20
upper jaw alveolar sulcus
strong strong
regionalstrong expression: see section 07 11 12
palatal shelf
strong strong
regionalstrong expression: see section 07 08 13
axial skeleton
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 14 15 16
basisphenoid bone
strong strong
regionalstrong expression: see section 09 10 11 12
exoccipital bone
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
frontal bone primordium
strong strong
regionalstrong expression: see section 01 02 23
parietal bone
strong strong
regionalstrong expression: see section 01 02
orbito-sphenoid
strong strong
regionalstrong expression: see section 03 18 19 20 21 22 23
clavicle
strong strong
regionalstrong expression: see section 03 04 05 06 07 14 15 16 17 18 19
scapula
strong strong
regionalstrong expression: see section 01 02 20 21 22
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 09 10 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36782
Entity Detected:Phospho1, phosphatase, orphan 1 ( MGI:2447348)
Sequence:sense strand is shown

>T36782
TATACTGACAATGAGCGGGTGTTTTCCAGCTGTTGGACTACGATGCCTATCTAGGGACGGCAGGATGGCT
GCGCCGGGCGCGCCTCGCTTCCTCCTCACCTTCGACTTCGATGAGACCATCGTGGACGAGAACAGCGACG
ACTCGATCGTGCGTGCCGCGCCAGGCCAGCAACTGCCCGAGAGCCTGCGTGCCACCTATCGCGAGGGCTA
CTACAATGAGTACATGCAACGAGTCTTTAAGTACCTGGGTGAGCAGGGTGTACGGCCCCGGGACCTGCGC
GCTGTCTACGAGACCATCCCCCTGTCGCCAGGCATGGGCGATTTGTTGCAGTTCATAGCCAAACAGGGCT
CCTGCTTCGAGGTTATTCTCATTTCGGATGCCAACACCTTCGGTGTGGAGAGTGCCCTGCGTGCCGCTGG
CCACCACAGTTTATTCCGCCGCATCCTCAGCAACCCGTCGGGGCCCGACGCGCGGGGACTGCTGACGCTG
CGGCCCTTCCACACGCACAGCTGCTCGCGCTGCCCGGCCAACATGTGCAAGCACAAGGTGCTCAGCGAAT
ACCTGCGTGAGCGGGCCCGCGACGGCGTGCACTTCGAGCGCCTCTTCTAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101959. Forward Primer - name:101959_F_cDNA_Phospho1, sequence:TATACTGACAATGAGCGGGTGT; Reverse Primer - name:101959_N_SP6_cDNA_Phospho1, sequence:GTAGAAGAGGCGCTCGAAGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8352 same embryo
 EMAGE:8357 same embryo
 EMAGE:8354 same embryo
 EMAGE:8353 same embryo
 EMAGE:8356 same embryo
 EurExpress:euxassay_007708 same experiment
 MGI:4827192 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS