EMAGE mouse gene expression spatial database

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EMAGE:8560

Dach2 dachshund 2 (Drosophila) ( MGI:1890446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009795. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009795_01	euxassay_009795_02	euxassay_009795_03	euxassay_009795_04

euxassay_009795_05	euxassay_009795_06	euxassay_009795_07	euxassay_009795_08	euxassay_009795_09

euxassay_009795_10	euxassay_009795_11	euxassay_009795_12	euxassay_009795_13	euxassay_009795_14

euxassay_009795_15	euxassay_009795_16	euxassay_009795_17	euxassay_009795_18	euxassay_009795_19

euxassay_009795_20	euxassay_009795_21	euxassay_009795_22	euxassay_009795_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8560_wholemount_strong.wlz

8560_wholemount_moderate.wlz

8560_wholemount_weak.wlz

8560_wholemount_possible.wlz

8560_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8560_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 13 14

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 11 15 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

weak

regional

weak expression: see section 18

rest of cerebellum marginal layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 20

pons mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21

tegmentum

weak

regional

weak expression: see section 11 13

basal columns

weak

regional

weak expression: see section 11 12 13 14 15

ear

strong

regional

strong expression: see section 05 06 20

urinary system

strong

regional

strong expression: see section 04 20

bladder

strong

regional

strong expression: see section 13 14 moderate expression: see section 12

urachus

strong

regional

strong expression: see section 13

renal medullary interstitium

weak

regional

weak expression: see section 07 08 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30636
Entity Detected:	Dach2, dachshund 2 (Drosophila) ( MGI:1890446)
Sequence:	sense strand is shown >T30636 GGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTAATGGAAGCCAAAATGGG ACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGA TACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCACTTGCTGGTCAGCCAGG CCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCAATCGTCTGGATCTCCCA TTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGCAATGAATC AGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCACAGTCCACTCTCCAGAGC TGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCTCTCTGGAAGAGAGTCAT CGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCCCTCGCAGATGGATCATC ATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGACCAAGATAATGGACAAAG CATAAAAAAATTCCAGAGGGATAATAAAGAAGAAGTACCAGCTCAAATCCCAGTCATGAAGTCACCCTTG GATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGATTCCCTGGACCATTCATTTTTGCAGATA GTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGGTCTACTGAAAGTGGCTTTGGATAATGCTCG TATCCAGGAGAAGCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCTTCAGAGAAAGAGAAATT AGAGAAAACCTGGAGCGACAACTTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6406445), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59630. Forward Primer - name:059630_F_IRAV115_h11_Dach2, sequence:GGCCATGAAGCTCCAGAA; Reverse Primer - name:059630_R_SP6_IRAV115_h11_Dach2, sequence:TGCAAGTTGTCGCTCCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8559 same embryo
	EMAGE:8564 same embryo
	EMAGE:8561 same embryo
	EMAGE:8563 same embryo
	EMAGE:8562 same embryo
	EurExpress:euxassay_009795 same experiment
	MGI:4824220 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source