EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9945

Wdr11 WD repeat domain 11 ( MGI:1920230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000103. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000103_01	euxassay_000103_02	euxassay_000103_03	euxassay_000103_04

euxassay_000103_05	euxassay_000103_06	euxassay_000103_07	euxassay_000103_08	euxassay_000103_09

euxassay_000103_10	euxassay_000103_11	euxassay_000103_12	euxassay_000103_13	euxassay_000103_14

euxassay_000103_15	euxassay_000103_16	euxassay_000103_17	euxassay_000103_18	euxassay_000103_19

euxassay_000103_20	euxassay_000103_21	euxassay_000103_22	euxassay_000103_23	euxassay_000103_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9945_wholemount_strong.wlz

9945_wholemount_moderate.wlz

9945_wholemount_weak.wlz

9945_wholemount_possible.wlz

9945_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9945_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

organ system

weak

homogeneous

weak expression: see section 11

submandibular gland primordium

moderate

regional

moderate expression: see section 19 20

vibrissa

moderate

regional

moderate expression: see section 22 23 weak expression: see section 24

cranial ganglion

moderate

regional

moderate expression: see section 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 19 23 weak expression: see section 04 06 22 24

vagus x ganglion

weak

regional

weak expression: see section 06

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 17 18 19 weak expression: see section 09 11

otic capsule

weak

regional

weak expression: see section 24

metanephros

weak

homogeneous

weak expression: see section 10 22

metanephros

moderate

regional

moderate expression: see section 23

renal cortex

weak

homogeneous

weak expression: see section 10 11

frontal bone primordium

weak

regional

weak expression: see section 01 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T252
Entity Detected:	Wdr11, WD repeat domain 11 ( MGI:1920230)
Sequence:	sense strand is shown >T252 GGGGAGGGCCAGGGTGCCGCCGCGATGTTACCCTACACCGTAAACTTCAAGGTGTCAGCGCGCACCCTCA CCGGGGCTCTCAACGCGCACAACAAGGCGGCGGTGGACTGGGGCTGGCAAGGTTTAATTGCATATGGATG CCATTCACTGGTGGTAGTGATCGATTCCAATACTGCCCAAACTCTACAAGTTCTAGAAAAACATAAAGCT GATATTGTTAAGGTCAGGTGGGCCAGGGAGAACTATCACCACAACATCGGCTCTCCATACTGCCTGCGCT TGGCTTCTGCGGATGTCACTGGAAAGATCATTGTCTGGGATGTAGCAGCCGGAGTAGCCCAGTGTGAGAT CCAAGAGCACGTGAAGCCCATCCAAGATGTGCAGTGGCTGTGGAATCAGGACGCTTCCCGAGACTTGCTG CTCGCTATCCACCCGCCTAATTACATCGTGCTGTGGAATGCAGACACGGGCACCAAGCTGTGGAAGAAGA GCTACGCAGATAACATCCTTTCTTT
Notes:	The probe template was PCR amplified from IMAGE:2651629 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2651629 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9946 same embryo
	EMAGE:9944 same embryo
	EurExpress:euxassay_000103 same experiment
	MGI:4829192 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source