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EMAGE:10215

Ccrl1 chemokine (C-C motif) receptor-like 1 ( MGI:2181676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215
"Pseudo-wholemount" of euxassay_000076. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000076_01 euxassay_000076_02 euxassay_000076_03 euxassay_000076_04
EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215
euxassay_000076_05 euxassay_000076_06 euxassay_000076_07 euxassay_000076_08 euxassay_000076_09
EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215
euxassay_000076_10 euxassay_000076_11 euxassay_000076_12 euxassay_000076_13 euxassay_000076_14
EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215
euxassay_000076_15 euxassay_000076_16 euxassay_000076_17 euxassay_000076_18 euxassay_000076_19
EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215 EMAGE:10215
euxassay_000076_20 euxassay_000076_21 euxassay_000076_22 euxassay_000076_23 euxassay_000076_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10215Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10215_wholemount_strong.wlz
10215_wholemount_moderate.wlz
10215_wholemount_weak.wlz
10215_wholemount_possible.wlz
10215_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10215_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
spottedweak expression: see section 12
cerebral cortex
moderate moderate
regionalmoderate expression: see section 04 weak expression: see section 02 06 11 16 17 20 21
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 04 weak expression: see section 03 09 10
cerebral cortex marginal layer
moderate moderate
regionalmoderate expression: see section 04 12 22 weak expression: see section 03 08 09 11
cerebral cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05 12 22 23 weak expression: see section 03 06 08 09 10 11
cerebellum
moderate moderate
regionalmoderate expression: see section 03
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 04
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 04
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 22 weak expression: see section 05 06 07 08 09 18 19 20 21
spinal cord
weak weak
homogeneousweak expression: see section 15
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 07 08 09 10 11 12 17 18
heart
weak weak
regionalweak expression: see section 05 06 11 19 20
lung
weak weak
spottedweak expression: see section 06 07 08 09 14 15 16 18 19 20 21
upper respiratory tract
weak weak
regionalweak expression: see section 05
nucleus pulposus
weak weak
regionalweak expression: see section 10 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1688
Entity Detected:Ccrl1, chemokine (C-C motif) receptor-like 1 ( MGI:2181676)
Sequence:sense strand is shown

>T1688
TGGCCTCGAGCCAGATTCGGACGAGGNTCCTATAACGTTGTTAAGTTCTGCCAAGCCATAGATGCCATCT
ACCTGCTGATCACCAGCTGCGATATGAGCAAACGCATGGATGTCGCCATCCAACTCACAGAGAGCATCGC
GCTCTTCCACAGCTGCCTCAACCCCATCCTGTATGTCTTCATGGGGGCCTCCTTCAAAAACTATATCATG
AAAGTGGCCAAGAAATATGGATCCTGGAGAAGACAGAGACAGAACGTGGAAGAAATTCCTTTTGATTCTG
AGGGTCCTACAGAGCCAACCAGTTCTTTTACCATTTAAATATAAAACTGCTCTCTGCCTCTGCTTTTTGC
TTAGACACACACACACACACACACACACACACACACACACAAAGTGATGTTTCCTCCTCAAATAAAATAT
TTGTGTTACACCGAGACCCAACCATCAACAAATACTGTTGTTGCAATTTATAACAAAGAAGGAGTAAGGG
GTGGGGCAGGGTAAGGAAGACAGACGCCAGAGAGTAAACAAGTGGGCAAAGTGTGGAAACT
Notes:The probe template was PCR amplified from IMAGE:476044 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:476044 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10214 same embryo
 EurExpress:euxassay_000076 same experiment
 MGI:4823715 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS