EMAGE mouse gene expression spatial database

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EMAGE:10672

9130017N09Rik RIKEN cDNA 9130017N09 gene ( MGI:1926156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000897. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000897_01	euxassay_000897_02	euxassay_000897_03	euxassay_000897_04

euxassay_000897_05	euxassay_000897_06	euxassay_000897_07	euxassay_000897_08	euxassay_000897_09

euxassay_000897_10	euxassay_000897_11	euxassay_000897_12	euxassay_000897_13	euxassay_000897_14

euxassay_000897_15	euxassay_000897_16	euxassay_000897_17	euxassay_000897_18	euxassay_000897_19

euxassay_000897_20	euxassay_000897_21	euxassay_000897_22	euxassay_000897_23	euxassay_000897_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10672_wholemount_strong.wlz

10672_wholemount_moderate.wlz

10672_wholemount_weak.wlz

10672_wholemount_possible.wlz

10672_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10672_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 not examined expression: see section 07

not examined

regional

not examined expression: see section 16 17

stomach

strong

regional

strong expression: see section 07 moderate expression: see section 10 11 weak expression: see section 06 08 09

hindgut

moderate

regional

moderate expression: see section 15 16 17 not examined expression: see section 07

midgut

strong

regional

strong expression: see section 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17

midgut loop

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2153
Entity Detected:	9130017N09Rik, RIKEN cDNA 9130017N09 gene ( MGI:1926156)
Sequence:	sense strand is shown >T2153 TGGCCTCGAGNCAGATTCGGACGAGGTGAATTCGGGGCTGGTGTGGAAGGTGGAGGCCCCTGAGGACAGT GCTCCCCCAGGGCAGAAGACTAGGAAGGAGATGTGGTATGCAGGTATCAACCCCTCAGACAGTGTCAACT CAGAGGTCCTGGGAGCCACAAGGGTAAAGCGGCACAAGAATCTCTTGGCGGAACGCTGGGAAGCACACAT CTATGCCAGCGAGGATGAAAACTGAGACTGGAAGCAGGGGGCCATTCTCCTGCCTCACTGCAGTCCTGTG GAGGCCGCTAGACCTGCCCCCAGTCTCTGCTGTCCCTATTTAAACATACAGCCCAGGCTGATGTTTGTGG CCTGCAGTTGATTTGGTTTTCCCTGGGGTGGGCGAGTGATTCGTGCTTAGGGGTGGTTCTCAGCTAAGGC TGAAATGATAATCCACCGATCCCCCTCACATCCCTCAGCCCTCCGCACAGCCCAGAGCTTGGATCTGTCA CTGCAGAAAGAAGGCTCTGTTTGGGGACCTGCCATTCCACTCTTGCCAACCCCAAAC
Notes:	The probe template was PCR amplified from IMAGE:875462 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:875462 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10670 same embryo
	EMAGE:10674 same embryo
	EMAGE:10669 same embryo
	EMAGE:10671 same embryo
	EMAGE:10673 same embryo
	EurExpress:euxassay_000897 same experiment
	MGI:4822797 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source