EMAGE mouse gene expression spatial database

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EMAGE:10890

B3galnt1 UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 ( MGI:1349405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003465. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003465_01	euxassay_003465_02	euxassay_003465_03	euxassay_003465_04

euxassay_003465_05	euxassay_003465_06	euxassay_003465_07	euxassay_003465_08	euxassay_003465_09

euxassay_003465_10	euxassay_003465_11	euxassay_003465_12	euxassay_003465_13	euxassay_003465_14

euxassay_003465_15	euxassay_003465_16	euxassay_003465_17	euxassay_003465_18	euxassay_003465_19

euxassay_003465_20	euxassay_003465_21	euxassay_003465_22	euxassay_003465_23	euxassay_003465_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10890_wholemount_strong.wlz

10890_wholemount_moderate.wlz

10890_wholemount_weak.wlz

10890_wholemount_possible.wlz

10890_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10890_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 10 11 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 10 11 19

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 21 moderate expression: see section 10 11 13 14 15 16 17 18 19 weak expression: see section 12 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5983
Entity Detected:	B3galnt1, UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 ( MGI:1349405)
Sequence:	sense strand is shown >T5983 CCCACGCGTCCGGACAGCCCCTGTGGTTAAGAACCTGTGCCTTCTGAACTTCTCTGCTGCTGTGGATGGC CCCGGCTGTCTTGACTGCCCTTCCCAATAGGATGTCACTGAGATCCCTCAAGTGGAGCCTGCTGCTGCTG TCCCTGCTGAGCTTCCTGGTGATATGGTACCTCAGCCTCCCCCACTACAATGTGATCGAGCGCGTCAACT GGATGTACTTCTATGAGTATGAGCCAATTTACAGACAAGACTTTCGCTTCACGCTCCGCGAACACTCGAA CTGTTCTCATCAGAACCCATTCCTGGTCATCCTGGTGACCTCACGCCCCTCAGATGTGAAAGCCAGACAA GCCATTAGAGTTACTTGGGGTGAGAAAAAGTCCTGGTGGGGATATGAGGTGCTCACGTTTTTCTTACTAG GCCAGCAGGCTGAAAGGGAAGACAAAACGTTAGCCCTGTCCTTGGAGGATGAGCACGTTCTCTATGGTGA TATTATACGGCAAGACTTTCTAGACACATATAATAACTTGACCTTGAAAACCATTATGGCTTTCAGGTGG GTAATGGAGTTTTGCCCCAATGCCAAGTATATTATGAAAACAGACACTGATGTTTTCATCAACACTGGCA ATTTAGTCAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACGGGCTATCCTCTAATTGATAA CTATTCCTATAGAGGATTTTTCCATAAAAACCACATTTCATACCAAGAGTACCCCTTCAAGGTGTTCCCT CCCTACTGCAGCGGGCTGGGCTACATTATGTCCGGCGACCTGGTGCCCAGGGTCTACGAGATGATGAGTC ACGTGAAGCCCATCAAGTTTGAAGACGTTTATGTTGGCATCTGTTTGAATTTGTTAAAAGTGGACATTCA TATTCCAGAAGACACAAACCTTTTCTTTCTGTACAGAATCCACTTGGATGTATGTCAGCTCAGACGCGTG ATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACATTCTGGCAGGTGATGCTGAGGAACACCACAT GCCATTACTAAGCCCATCCATCACCCATCTAGGCAAGGGAGGAAGGACACTGTAGACAGGGTCAGAGTTA GCACTGTGGGAAACTCGGGAAGTTGAGCGTGCTGGTTTGCCTGGGCTGAAACTCATGGAGCTCCCTAGAC AGGAGTCAAGGCCTGAACTTAGTGATTGCTTTCACAGAATTTAACTTGGGTCGTTTAAAGGTGACAGAGG AGTCAAACATAATGCAAACGAAGAGTTTTGCTAACCAAATCAAAGGGTCAGACAGTCTGGATGGCTCAGG CTGTAGATTAGAATTTCTTAAGATTTCCCTAAAAGAAAAACTAACTAGTCCACAGCCAGAATACAGTGTG GAGTTGTATTTATTAGACAGTATAGTTACATAATGGTTCAGTGTGTATCTTAAGTGGTTATTATGTAAAG ATATATATATATATATTTCTTGTAAAAAGCTTTACAGAGTTATATTGAAAACATTCATTTGTATTGTTTT CATTTGCAAGGTACTCAATGATATGGTACGTAAGAGTTATCAAATCAAGTATTATTTAATGTCATTTCAA TTTTCTAAATGTTTAAAGGTTATGTATGGTCTCAGTATTATATGATGAATTGCCTTTCACATTTGAGCTT TGTTTTATTCACCACTGATCAGTTTAATTAATGTGACGTTAATGTGACATCAAAGGTTATTACTGACTGT CCGTAATCTGTTGAACTCTGTTGCACTGTAAACATAGAGAATTAAAGCAAGAAAATTCAAGCTTTGTATA GTTTTTTAAAAATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3486016 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486016 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10894 same embryo
	EMAGE:10891 same embryo
	EMAGE:10893 same embryo
	EMAGE:10889 same embryo
	EMAGE:10892 same embryo
	EurExpress:euxassay_003465 same experiment
	MGI:4823370 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source