EMAGE mouse gene expression spatial database

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EMAGE:11910

Itga11 integrin alpha 11 ( MGI:2442114)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008538. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008538_01	euxassay_008538_02	euxassay_008538_03	euxassay_008538_04

euxassay_008538_05	euxassay_008538_06	euxassay_008538_07	euxassay_008538_08	euxassay_008538_09

euxassay_008538_10	euxassay_008538_11	euxassay_008538_12	euxassay_008538_13	euxassay_008538_14

euxassay_008538_15	euxassay_008538_16	euxassay_008538_17	euxassay_008538_18	euxassay_008538_19

euxassay_008538_20	euxassay_008538_21	euxassay_008538_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11910_wholemount_strong.wlz

11910_wholemount_moderate.wlz

11910_wholemount_weak.wlz

11910_wholemount_possible.wlz

11910_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11910_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 weak expression: see section 22

humerus

moderate

regional

moderate expression: see section 02 03 04 05 06 21 22

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 04 05 22

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 21 22

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 21 22

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 09 21 22

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 21 22

femur

moderate

regional

moderate expression: see section 20 weak expression: see section 07 08 09

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15

diencephalon meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

moderate

regional

moderate expression: see section 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19

mandible

moderate

regional

moderate expression: see section 04 05 06 07 08 20 21 weak expression: see section 03 09 10 11 16 17 18 19 22

maxilla

moderate

regional

moderate expression: see section 07 08 21 weak expression: see section 09 10 17 18

axial skeleton

strong

regional

strong expression: see section 11 12 13 14 15 16 moderate expression: see section 17

clavicle

moderate

regional

moderate expression: see section 10 11 16 17 18 19 weak expression: see section 08 09

scapula

moderate

regional

moderate expression: see section 03 04 05 22

pelvic girdle skeleton

moderate

regional

moderate expression: see section 11 12 18 19 20

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9302
Entity Detected:	Itga11, integrin alpha 11 ( MGI:2442114)
Sequence:	sense strand is shown >T9302 GGCTCAGGACGCTCGCGAACCGAGGCTGTGGCTCTGGCTCTCAGCCGCGCGACCATCGCGACACCCGCCG CCGCGCAGCGGGCCATGGACTTTCCCAGGGGCCTCCTGGTGGCCTGGACCCTCAGCCTTTGGCCAGGATT CACGGACACCTTCAATATGGATACCAGAAATCCCCGGGTCATTGCTGGTCCCAGTGCTGCCTTCTTTGGC TACACAGTACAGCAGCATGATATCAGTGGCAAGAAGTGGTTGGTTGTGGGTGCCCCAATGGAAACCAATG GCCATCAGAAGACAGGAGACGTATACAAATGTCCGGTAACCCAGGGCAACTGCACCAAGCTCAACCTGGG CAGGGTCACTCTGTCCAATGTGTCTGAGAGGAAGGACAACATGCGCCTCGGCCTGAGCCTTGCCACCAAC CCCAAGGACAACAGCTTCCTGGCCTGCAGCCCTCTCTGGTCGCACGAGTGTGGAAGCTCCTACTACACCA CTGGCATGTGCTCACGGGCCAACTCCAACTTCAGATTCTCTAAGACGGTAGCTCCGGCACTTCAGAGGTG CCAGACTTACATGGACATCGTCATTGTCTTAGATGGCTCCAACAGCATCTACCCCTGGGTGGAGGTCCAA CACTTCCTCATCAATATCCTCAAAAAGTTCTACATTGGCCCCGGCCAGATCCAGGTCGGAATAGTCCAGT ATGGAGAAGATGCCGTCCATGAGTTCCACCTTAATGACTACAGGTCTGTAAAAGATGTGGTGGAAGCCGC CAGCCACATTGAGCAGAGAGGAGGGACAGAGACCCGCACGGCATTTGGCATTGAATTTGCACGCTCGGAG GCTTTCCAGAAGGGTGGAAGAAAAGGGGCCAAGAAAGTGATGATTGTCATCACGGACGGGGAATCCCACG ACAGCCCAGACCTGGAGAAGGTGATCCGGCAGAGCGAGAAGGACAACGTGACCAGATACGCTGTGGCCGT TTTGGGCTACTACAACCGCAGGGGGATCAATCCAGAGACTTTTCTAAATGAAATCAAATACATCGCCAGC GACCCTGACGACAAGCACTTCTTCAACGTCACAGATGAGGCGGCCCTGAAGGACATTGTTGATGCCCTTG GGGACAGGATCTTCAGCTTGGAAGGCACCAACAAGAATGAGACCTCTTTTGGGCTGGAGATGTCGCAGAC TGGCTTTTCTTCACATGTGGTAGAGGATGGGATCCTGCTGGGAGCTGTGGGAGCCTATGACTGGAACGGG GCGGTGCTGAAGGAGACAAGTGCTGGCAAGGTGATTCCTCACCGAGAGTCCTACCTTAAGGAGTTTCCAG AGGAGCTGAAGAACCATGCAGCATACCTAGGGTACACGGTGACGTCGGTTGTGTCCTCCAGGCAGGGGCG GGTGTATGTGGCTGGAGCCCCCAGATTCAACCATACTGGCAAGGTCATTCTATTCAGCATGCACAACAAC CGGAGCCTCACCATCCACCAGGCTCTTCGGGGCGAGCAGATAGGCTCCTACTTTGGGAGTGAGATTACCT CAGTGGACGTCAATGATGACAGAGTGACAGATGTGCTGCTGGTGGGTGCACCCATGTACTTCAGCGAGGG CCGAGAGCGAGGCAAGGTGTATGTCTACAACCTGAGACAGAACCGGTTTGTTTATAATGGCACTCTGAAG GATTCCCACAGCTACCAGAACGCCCGGTTCGGGTCCTGCATCGCCTCTGTTCAAGACCTCAACCAAGATT CCTACAATGATGTGGTGGTGGGGGCCCCTCTGGAGGACAGCCACAGAGGGGCCATCTACATCTTCCATGG CTTCCAAACCAATATCCTGAAGAAGCCCATGCAGAGAATAACAGCCTCGGAGCTGGCTCCTGGCCTGCAG CATTTTGGCTGCAGCATCCACGGACAACTGGACCTCAATGAGGATGGGCTTGTGGACCTAGCAGTGGGCG CCCTGGGCAACGCTGTGGTTTTGTGGGCCCGCCCTGTAGTTCAAATCAACGCCAGCCTCCACTTTGAGCC TTCCAAGATCAACATCTTCCACAAGGACTGCAAGCGCAATGGCAGGGATGCCACCTGCCTGGCCGCCTTC CTCTGCTTCATACCCATCTTCCTGGCACCCCACTTCCAAACAGCAACCGTCGGCATCAGGTACAATGCAA CCATGGATGAGAGGCGGTATATGCCGCGGGCACATCTGGACGAGGGCGGAGACCAGTTCACCAACAGAGC CGTCCTGCTCTCCTCTGGCCAGGAACACTGTCAAAGGATCAACTTCCATGTCCTGGACACTGCAGACTAC GTGAAGCCAGTGGCCTTCTCCGTGGAGTACTCCCTAGAGGACCCTGACAACGGCCCCATGCTGGACAACG GCTGGCCCACTACGCTCAGAGTGTCGGTGCCCTTCTGGAATGGCTGTAATGAGGATGAACACTGTGTCCC TGACCTTGTACTGGATGCTCGGAGTGACCTGCCCACTGCCATGGAGTACTGCCAGCGGGTGCTGGGGAGG CCGGCGCAGGACTGCTCCAGCTACACCCTGTCCTTCGACACCACTGTCTTCATCATAGAGAGCACGCGCC GCCGGGTGGCAGTGGAGGCCACGCTGGAGAACAGAGGAGAGAATGCTTACAGCGCCGTCCTCAACATCTC CCAGTCAGAGAACCTTCAGTTTGCCAGCCTGATCCAGAAGGATGACTCAGACAACAGCATCGAGTGTGTG AACGAGGAGAGGCGGCTTCACAAGAAAGTCTGCAACGTCAGCTACCCCTTCTTCAGAGCCAAGGCCAAGG TGGCTTTCCGTCTGGATTTCGAGTTCAGCAAGTCTGTGTTTCTGCACCATCTTCAGATCCATCTGGGTGC CGGCAGTGACAGTCACGAGCAAGACAGCACCGCAGACGATAACACGGCCCTCCTGCGCTTCCACCTCAAA TATGAAGCAGACGTCCTCTTTACCAGAAGCAGCAGCCTGAGCCACTTCGAGGTCAAGGCAAACAGCTCAC TTGAGAGCTATGATGGCATCGGACCTCCGTTCAACTGTGTTTTCAAGGTGCAGAACCTGGGCTTTTTCCC CATCCACGGGGTGATGATGAAGATCACTGTGCCCATCGCCACCAGGGGTGGCAACCGCCTGCTGATGTTG AGGGACTTCTTCACCGACCAGGGCAACACATCCTGTAACATCTGGGGGAACAGCACAGAGTACAGGAGTA CCCCAACTGAGGAAGATCTGAGCCATGCCCCACAGAGGAATCACAGCAACTCCGACGTGGTCTCCATCAT CTGCAACCTGAGGCTGGCCCCCAGCCAGGAGACCAGCTTCTACCTGGTGGGAAACCTGTGGCTGACATCT CTGAAAGCACTCAAGTACAGGTCTCTGAAGATCACAGTCAACGCCGCCTTGCAGAGGCAGTTCCACAGCC CCTTCATCTTCCGAGAGGAAGACCCTAGCCGCCAGGTCACATTTGAGATCTCCAAGCAAGAAGACTGGCA GGTCCCCATCTGGATCATCGTGGGCAGCACTCTGGGGGGCCTCTTGCTGCTGGCCCTGCTGGTCCTGGCA CTGTGGAAGCTCGGTTTTTTTAAAAGTGCCAAGCGCAAGAGGGAACCTGGCCTAGGCCCCATCCCCAAAG AGCTCAAGTGAGGATCCAGAGGAAGCTTCAAGTTGTTGGGGGCCCGACCAGTCCGGGGGTGTGCAGGCCC GGGCTATGGCCTCAGAGCTGGAGCAAAGAGGACACCCATGGACTTTACATTGAGTTCATCTCTGGAGCAA TGGCAACTGATCGCTGTGGGATGGACCTCCAGCCCGTGTTCGGAGTGACTTCTCTACTAGAGGCCAAGAC CTCCAGTACAGATCCCTAAGGACTAAAAGGGACCCTCCAAGACCCACCGGTGGCCTTCCCCCAAGGCTCT GTGGAGGGAATCTGCTGCCCCAAACACTAAGGTGCTAGGGTTTTGTTGTCTTGCCCACCTCTGAAGAAAC TCAAGGGGAAGCTAGCAAATACGAGCCCACTGTGCACGCCCTATCTGGACCTATAGCTTCAGGACTCCAT GGCCCGGGAGCATAACCTCCGCCGCACCCCTTACCCTTGCTCTTGGTTCCCAAACCACAGCCATCCCTTC TCCACAGATAGACCCCAAAGATCTCTATGAGTGACCCAGAGTTGTGTGGCTTGATATGCCAGCTGCAGGC CAGTGATGGAAACACATCCAGGGACTGTGCCAAATGAACTGGAGGGAATCAGGATGCTGGGTACCTTCCA AACATCAGAAAGCAGCAGGGCTCTCCAGCAGTAACCACCACCCACTCAATCCTGCTCTCCAGCCTCCCCC GCAGCTCCTCTCAGCCGCTTTGGTTGCTAGGCAACTCGTGCTAGGGAGCCTGAGGAGGATGATAAAAATC CGTGGCTCTCCGTACCCTCTTGTCTGACAACTGCAGCTTGTCCCTGGTAGATGCACTGTGATAGGGAGCC TCTGTTTCTTACATCAGCACCTACACACACACACACACACACACACACACACACACACACACACACACAC GCACGCACCTCCCAACCATCAGCGTGTGGGAGAAGGGCTCCTGTAGGGGGCGCTGTGGGGGGCCTTCTAG GCATGCACTGAACAAAGCCCATGCAAGGCCCCTGGGAACCTTAAACCTCAGAGGCAGATAGGCATCTCGC CGGCCAGCTCTGGGATAAGCCCCAAGGACAAGGGGCATGCCGGATGCCCAGCAGAGTAATTTATGCCTTA ACCTTTTGTTGAGATGGAAATGGACACATCAGTGTCCCCTGTCATCTGTTTCATCTGAACAATGTCATCT GTTCCCCTATGCGGTACGACCTTTACTGTCAGAAATATATTTAAGAAAATGTTCTAAACGGTATTTAAAA ACAACCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6842988 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6842988 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11912 same embryo
	EMAGE:11908 same embryo
	EMAGE:11909 same embryo
	EMAGE:11913 same embryo
	EMAGE:11911 same embryo
	EurExpress:euxassay_008538 same experiment
	MGI:4825638 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source