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EMAGE:12047

Galr1 galanin receptor 1 ( MGI:1096364)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12047 EMAGE:12047 EMAGE:12047 EMAGE:12047 EMAGE:12047
"Pseudo-wholemount" of euxassay_008370. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008370_01 euxassay_008370_02 euxassay_008370_03 euxassay_008370_04
EMAGE:12047 EMAGE:12047 EMAGE:12047 EMAGE:12047 EMAGE:12047
euxassay_008370_05 euxassay_008370_06 euxassay_008370_07 euxassay_008370_08 euxassay_008370_09
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euxassay_008370_10 euxassay_008370_11 euxassay_008370_12 euxassay_008370_13 euxassay_008370_14
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euxassay_008370_15 euxassay_008370_16 euxassay_008370_17 euxassay_008370_18 euxassay_008370_19
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euxassay_008370_20 euxassay_008370_21 euxassay_008370_22 euxassay_008370_23 euxassay_008370_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12047Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12047_wholemount_strong.wlz
12047_wholemount_moderate.wlz
12047_wholemount_weak.wlz
12047_wholemount_possible.wlz
12047_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12047_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 08 09 16
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 09 10 13 14 15 17 18 weak expression: see section 08 11 12 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35170
Entity Detected:Galr1, galanin receptor 1 ( MGI:1096364)
Sequence:sense strand is shown

>T35170
TCATTACGCTGGTAGTGTTTGGCCTGATTTTCGCGATGGGCGTGCTGGGCAACAGCCTGGTGATCACCGT
GCTGGCGCGCAGCAAACCAGGCAAGCCGCGCAGCACCACCAACCTGTTTATCCTCAATCTGAGCATCGCA
GACCTGGCCTACCTGCTCTTCTGCATCCCTTTTCAGGCCACCGTGTATGCACTGCCAACCTGGGTGCTGG
GCGCCTTCATCTGCAAGTTTATACACTACTTCTTCACCGTGTCCATGCTGGTGAGCATCTTCACCCTGGC
CGCGATGTCTGTGGATCGCTACGTGGCCATTGTGCACTCGCGGCGCTCCTCCTCCCTCAGGGTGTCCCGC
AACGCACTGCTGGGCGTGGGCTTCATCTGGGCGCTGTCCATCGCCATGGCCTCGCCGGTGGCCTACCACC
AGCGTCTTTTCCATCGGGACAGCAACCAGACCTTCTGCTGGGAGCAGTGGCCCAACAAGCTCCACAAGAA
GGCTTACGTGGTGTGCACTTTCGTCTTTGGGTACCTTCTGCCCTTACTGCTCATCTGCTTTTGCTATGCC
AAGGTCCTTAATCATCTGCATAAAAAGCTGAAAAACATGTCAAAAAAGTCTGAAGCATCCAAGAAAAAGA
CTGCACAGACCGTCCTGGTGGTCGTTGTAGTATTTGGCATATCCTGGCTGCCCCATCATGTCGTCCACCT
CTGGGCTGAGTTTGGAGCCTTCCCACTGACGCCAGCTTCCTTCTTCTTCAGAATCACCGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89546. Forward Primer - name:089546_F_cDNA_Galr1, sequence:TCATTACGCTGGTAGTGTTTGG; Reverse Primer - name:089546_N_SP6_cDNA_Galr1, sequence:GGCGGTGATTCTGAAGAAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12046 same embryo
 EMAGE:12049 same embryo
 EMAGE:12045 same embryo
 EMAGE:12048 same embryo
 EurExpress:euxassay_008370 same experiment
 MGI:4824990 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS