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EMAGE:12805

Ckb creatine kinase, brain ( MGI:88407)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805
"Pseudo-wholemount" of euxassay_011493. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011493_01 euxassay_011493_02 euxassay_011493_03 euxassay_011493_04
EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805
euxassay_011493_05 euxassay_011493_06 euxassay_011493_07 euxassay_011493_08 euxassay_011493_09
EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805
euxassay_011493_10 euxassay_011493_11 euxassay_011493_12 euxassay_011493_13 euxassay_011493_14
EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805
euxassay_011493_15 euxassay_011493_16 euxassay_011493_17 euxassay_011493_18 euxassay_011493_19
EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805 EMAGE:12805
euxassay_011493_20 euxassay_011493_21 euxassay_011493_22 euxassay_011493_23 euxassay_011493_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12805Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12805_wholemount_strong.wlz
12805_wholemount_moderate.wlz
12805_wholemount_weak.wlz
12805_wholemount_possible.wlz
12805_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12805_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24
adrenal gland
strong strong
spottedstrong expression: see section 10 11 12 19 20 21
hypothalamus mantle layer
strong strong
single cellstrong expression: see section 08 09 10 11 13 14 15 16 17
hypothalamus ventricular layer
strong strong
homogeneousstrong expression: see section 11 12 13 14 15 16
diencephalon lateral wall mantle layer
strong strong
single cellstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
diencephalon lateral wall ventricular layer
strong strong
homogeneousstrong expression: see section 10 11 12 13
telencephalon mantle layer
strong strong
single cellstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24
olfactory cortex ventricular layer
strong strong
homogeneousstrong expression: see section 09 10 11 15 16 17
telencephalon ventricular layer
strong strong
homogeneousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
medulla oblongata basal plate mantle layer
strong strong
single cellstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16
rest of cerebellum ventricular layer
strong strong
homogeneousstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
pons mantle layer
strong strong
single cellstrong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18 19 20
pons ventricular layer
strong strong
homogeneousstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17
midbrain mantle layer
strong strong
single cellstrong expression: see section 06 07 08 09 10 11 13 14 15 16 17 18
midbrain ventricular layer
strong strong
homogeneousstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
facial vii ganglion
strong strong
regionalstrong expression: see section 04 05 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 09 19 20
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 19 20 21 moderate expression: see section 18
ventral grey horn
strong strong
single cellstrong expression: see section 12 13 14 15 16 17 18
dorsal root ganglion
strong strong
regionalstrong expression: see section 10 11 13 17 18 19 20 moderate expression: see section 12
neural retina
strong strong
homogeneousstrong expression: see section 01 02 03 04 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 15 16 17 18 19
vomeronasal organ
strong strong
regionalstrong expression: see section 12 13 15
esophagus
strong strong
regionalstrong expression: see section 13 14 15
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11
hindgut
strong strong
regionalstrong expression: see section 13 14 15
rectum
strong strong
regionalstrong expression: see section 13 14 15
midgut
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21
mandible
strong strong
single cellstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24
maxilla
strong strong
single cellstrong expression: see section 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23
bladder
strong strong
regionalstrong expression: see section 12 13 14 15 16
lung
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24 moderate expression: see section 04 13
sternum
strong strong
regionalstrong expression: see section 14 16 moderate expression: see section 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30969
Entity Detected:Ckb, creatine kinase, brain ( MGI:88407)
Sequence:sense strand is shown

>T30969
GGAATCCTCACCTGGGCTACATCCTCACATGCCCATCCAACCTGGGCACCGGACTGCGGGCAGGTGTGCA
CATCAAGCTGCCCCACCTGGGGAAGCACGAGAAGTTCTCGGAGGTGCTCAAGCGGCTGCGGCTTCAGAAG
CGAGGCACAGGTGGCGTGGACACCGCTGCTGTCGGTGGGGTGTTTGATGTCTCCAACGCTGACCGCCTGG
GCTTCTCGGAGGTGGAACTGGTGCAGATGGTGGTGGACGGAGTGAAACTACTCATTGAGATGGAGCAACG
GCTCGAGCAGGGTCAGGCAATCGATGACCTCATGCCGGCCCAGAAGTGAAGCCTGGCCCTCGCCACCATC
AGGCTGCCGCTTCCTAACTTATTACCCGGGCAGTGCCCGCCATGCATCCTTGATGTTTGCCGCCTGGCGG
CTGAGCCCTTAGCCTCGCTGTAGAGACTTCTGTCGCCCTGGGTAGAGTTTATTTTTTTGATGGCTAAGCT
GTTGCTGACACTGAAAATAAACTAGGGTTTGGCCTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4225384), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35354. Forward Primer - name:035354_F_IRAV28-31_N15_Ckb, sequence:GGAATCCTCACCTGGGCT; Reverse Primer - name:035354_R_SP6_IRAV28-31_N15_Ckb, sequence:GGCAGGCCAAACCCTAGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12806 same embryo
 EMAGE:12804 same embryo
 EurExpress:euxassay_011493 same experiment
 MGI:4823888 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS