EMAGE mouse gene expression spatial database

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EMAGE:14486

Pcgf1 polycomb group ring finger 1 ( MGI:1917087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005348. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005348_01	euxassay_005348_02	euxassay_005348_03	euxassay_005348_04

euxassay_005348_05	euxassay_005348_06	euxassay_005348_07	euxassay_005348_08	euxassay_005348_09

euxassay_005348_10	euxassay_005348_11	euxassay_005348_12	euxassay_005348_13	euxassay_005348_14

euxassay_005348_15	euxassay_005348_16	euxassay_005348_17	euxassay_005348_18	euxassay_005348_19

euxassay_005348_20	euxassay_005348_21	euxassay_005348_22	euxassay_005348_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14486_wholemount_strong.wlz

14486_wholemount_moderate.wlz

14486_wholemount_weak.wlz

14486_wholemount_possible.wlz

14486_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14486_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

regional

moderate expression: see section 05 06 07 13 14

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 09 14

cervical ganglion

moderate

regional

moderate expression: see section 08 15

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8273
Entity Detected:	Pcgf1, polycomb group ring finger 1 ( MGI:1917087)
Sequence:	sense strand is shown >T8273 CGTCTCCTCAGGGGGGCCAGATTGCGATCGCGATGAGGCTTCGGAACCAGCTCCAGTCAGTGTACAAGAT GGACCCACTACGGAACGAGGAGGAGGTCCGGGTGAAGATCAAAGACCTGAATGAACACATCGTTTGCTGC CTGTGCGCGGGCTACTTCGTGGATGCCACCACCATCACAGAGTGTCTCCACACCTTCTGCAAAAGTTGTA TCGTGAAGTACCTGCAAACCAGCAAGTACTGCCCCATGTGCAACATCAAGATCCACGAGACGCAGCCGTT GCTCAATCTCAAACTGGATCGGGTCATGCAGGACATAGTGTATAAGCTAGTGCCAGGCTTGCAAGACAGT GAAGAGAAACGGATTCGGGAATTCTATCAGTCCCGAGGCTTAGACAGAGTCTCCCAGCCCAGTGGTGAAG AGCCAGCCCTGAGCAACCTTGGCCTCCCCTTCAGCAGTTTTGACCACTCTAAAGCCCACTATTATCGATA TGATGAACAGCTGAGCCTATGCCTGGAGCGGCTGAGTTCTGGCAAAGACAAGAATAAAAATGTCCTTCAG AACAAGTATGTTCGGTGTTCTGTGAGAGCTGAGGTCCGCCATCTCCGAAGGGTTCTGTGTCACCGACTAA TGCTAAATCCACAGCATGTACAGCTCCTTTTTGACAATGAGGTTCTCCCAGATCACATGACAATGAAACA GCTATGGCTGTCCCGCTGGTTCGGCAAGCCATCTCCTTTGCTTCTCCAATACAGTGTGAAAGAGAAGAGG AGGTAGGGGCCAGGCTTGCTTCCACCCCCTTCCCACCCCTCCCCAGATATTTATGTGAAATTAACTGTGG CTTTATTTTTTGAAATAAATGCTTTTAAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1380201 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1380201 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14489 same embryo
	EMAGE:14488 same embryo
	EMAGE:14487 same embryo
	EMAGE:14490 same embryo
	EMAGE:14485 same embryo
	EurExpress:euxassay_005348 same experiment
	MGI:4827093 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source