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EMAGE:15987

Ccdc108 coiled-coil domain containing 108 ( MGI:2444274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987
"Pseudo-wholemount" of euxassay_013426. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013426_01 euxassay_013426_02 euxassay_013426_03 euxassay_013426_04
EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987
euxassay_013426_05 euxassay_013426_06 euxassay_013426_07 euxassay_013426_08 euxassay_013426_09
EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987
euxassay_013426_10 euxassay_013426_11 euxassay_013426_12 euxassay_013426_13 euxassay_013426_14
EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987
euxassay_013426_15 euxassay_013426_16 euxassay_013426_17 euxassay_013426_18 euxassay_013426_19
EMAGE:15987 EMAGE:15987 EMAGE:15987 EMAGE:15987
euxassay_013426_20 euxassay_013426_21 euxassay_013426_22 euxassay_013426_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15987Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15987_wholemount_strong.wlz
15987_wholemount_moderate.wlz
15987_wholemount_weak.wlz
15987_wholemount_possible.wlz
15987_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15987_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 13 14 15 18 19 weak expression: see section 11 12 16 17
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 03 04 05 07 08 09 10 13 14 15 18 19 weak expression: see section 06 11 12 16 17 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45305
Entity Detected:Ccdc108, coiled-coil domain containing 108 ( MGI:2444274)
Sequence:sense strand is shown

>T45305
CCTGAGTCTCACTTCTGTGTGGTCTTCCCCCTTGCCTCCCCCCAGGGAAGAACTCAGCTGGGATCAAACC
CTTTGGAAAAACTTAGTGCCTAGGGAGGAGCCCTCTGGGGGGTGTCAGGTAGGGGTCCCTGGTCACTAGG
CCTTAAAGAAGGTACCCAAAGCAAAAACATTGTTTTTATTTTGTAGTTTGTAGGGTTTTTTTGTTTTCTG
TATTTGTTTTTGTTTTTGTTTTTGTTTTGTTTTGTTTTGCTTTTCAAGACAGGGTTTCTTTCACTATACA
GCCAGGGTTGGCCTCAACTCCTTGCAATCTCCTTGCTTCAGCCTCCCATTTCTGAGACTACAGATACAAA
CTCACAACTTGCTGCTCAAAGCATGGTTGTGGGGTTTTGGTTTTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 191974. Forward Primer - name:191974_F_exon_B230363K08Rik, sequence:CCTGAGTCTCACTTCTGTGTGG; Reverse Primer - name:191974_N_SP6_exon_B230363K08Rik, sequence:ACAAAACCAAAACCCCACAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15988 same embryo
 EMAGE:15989 same embryo
 EMAGE:15984 same embryo
 EMAGE:15985 same embryo
 EMAGE:15986 same embryo
 EurExpress:euxassay_013426 same experiment
 MGI:4823662 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS