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EMAGE:18497

Rapgef2 Rap guanine nucleotide exchange factor (GEF) 2 ( MGI:2659071)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497
"Pseudo-wholemount" of euxassay_014449. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014449_01 euxassay_014449_02 euxassay_014449_03 euxassay_014449_04
EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497
euxassay_014449_05 euxassay_014449_06 euxassay_014449_07 euxassay_014449_08 euxassay_014449_09
EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497
euxassay_014449_10 euxassay_014449_11 euxassay_014449_12 euxassay_014449_13 euxassay_014449_14
EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497
euxassay_014449_15 euxassay_014449_16 euxassay_014449_17 euxassay_014449_18 euxassay_014449_19
EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497 EMAGE:18497
euxassay_014449_20 euxassay_014449_21 euxassay_014449_22 euxassay_014449_23 euxassay_014449_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18497Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18497_wholemount_strong.wlz
18497_wholemount_moderate.wlz
18497_wholemount_weak.wlz
18497_wholemount_possible.wlz
18497_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18497_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 15 17 weak expression: see section 09 10 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37968
Entity Detected:Rapgef2, Rap guanine nucleotide exchange factor (GEF) 2 ( MGI:2659071)
Sequence:sense strand is shown

>T37968
TTCTTCTCCCCTTCTTCCTTTCCCCTTTGCATGTGAAATACTGTGAAGAAATTGCCCTGGCACTTTGCAG
ACTTGTTGCTTGAAATGCACAGCCCAGCAGCCCCTGAGCTGCTGCCTGCCACGTCACGCAGTATCATTCC
AAATTCCAAGATCGTCACAAGGCGATTCCCTCTGGCGGCCCTTCTCCATGCCTGGAAGGAGATTCCAATC
TTCCTCTTAGATTTCAATCCAGTCCTAGCCCTCGATCTCATTTGGAAGATGAGAAAAGCTAGCCTTTGAA
CTACCCGGGGTCTCGAACCCACCAAGGAAGACAAAGACTGATATGAAATCCTTTGAGTACAGTGCTTGTC
CACTTGTTTACAATATCCTTTAAAAAAAAATAAGTTTAAAGATTGTGTTCAGAGAGTAAATGTTATATCC
ATTTTATGATTACAGTATTATTTTGAACCTTAAGTAGGATTGCCAGCCTGGGTTTCTGAAAAACCAAATA
TGCCGGACAGGGTTTGGCCGCACCAAGGGAAGACCTGGGCAGTGACCCTGTCTTCCCATGTCCTTCTGGC
CTTGCCCGTGAAGTGCCCTATCCCAGAAGCATCAAACGTTAGCCAATTACTTAAATACCAAGACTCCTCA
CCTGCCCCTTCCCCAGTGGGTGAGGCTCTAATGTAAAACAGTTTGCACATGGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77247. Forward Primer - name:077247_F_cDNA_Rapgef2, sequence:TTCTTCTCCCCTTCTTCCTTTC; Reverse Primer - name:077247_N_SP6_cDNA_Rapgef2, sequence:GGCCATGTGCAAACTGTTTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18499 same embryo
 EMAGE:18498 same embryo
 EMAGE:18495 same embryo
 EMAGE:18496 same embryo
 EMAGE:18494 same embryo
 EurExpress:euxassay_014449 same experiment
 MGI:4827611 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS