Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18593

Plce1 phospholipase C, epsilon 1 ( MGI:1921305)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593
"Pseudo-wholemount" of euxassay_014545. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014545_01 euxassay_014545_02 euxassay_014545_03 euxassay_014545_04
EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593
euxassay_014545_05 euxassay_014545_06 euxassay_014545_07 euxassay_014545_08 euxassay_014545_09
EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593
euxassay_014545_10 euxassay_014545_11 euxassay_014545_12 euxassay_014545_13 euxassay_014545_14
EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593
euxassay_014545_15 euxassay_014545_16 euxassay_014545_17 euxassay_014545_18 euxassay_014545_19
EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593 EMAGE:18593
euxassay_014545_20 euxassay_014545_21 euxassay_014545_22 euxassay_014545_23 euxassay_014545_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18593Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18593_wholemount_strong.wlz
18593_wholemount_moderate.wlz
18593_wholemount_weak.wlz
18593_wholemount_possible.wlz
18593_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18593_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon floor plate
strong strong
regionalstrong expression: see section 12
medulla oblongata floor plate
strong strong
regionalstrong expression: see section 10 11
metencephalon floor plate
strong strong
regionalstrong expression: see section 11 12
midbrain floor plate
strong strong
regionalstrong expression: see section 11 12
spinal cord floor plate
strong strong
regionalstrong expression: see section 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38580
Entity Detected:Plce1, phospholipase C, epsilon 1 ( MGI:1921305)
Sequence:sense strand is shown

>T38580
AAGAGCCTACTCTTTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCCATCAGTGCC
TGGAGCAGCAGCAGCTGGCATGGGCGAATCCGAGGAGGAATGCAAGGCTTCCAGAGCTTCATGGTCTCAG
ACAGTAACATGAGTTTCGTTGAATTCGTCGAGCTGTTCAAATCATTCAGCATCAGGAGCCGCAAGGACTT
GAAGGACATCTTTGACATCTACTCTGTACCCTGCAATCGGTCTGCCTCGGAGTCAGCCCCACTCTACACC
AACCTGACAATCGAAGAAAATACCAGCGATCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCCG
ACCTGGGGCTGTTCATTAAAAGTAAACAGCAGCTTTCTGACAACCAGAGGCAGATATCTGATGCTATCGC
TGCTGCAAGCATCGTGACCAATGGTACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCATA
CTCCAGCTCAATGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 148421. Forward Primer - name:148421_F_cDNA_Plce1, sequence:AAGAGCCTACTCTTTGACCACG; Reverse Primer - name:148421_N_SP6_cDNA_Plce1, sequence:TCATCATATGTGCAGTGTTCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18590 same embryo
 EMAGE:18589 same embryo
 EMAGE:18591 same embryo
 EMAGE:18592 same embryo
 EurExpress:euxassay_014545 same experiment
 MGI:4827254 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS