EMAGE mouse gene expression spatial database

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EMAGE:18721

Slamf1 signaling lymphocytic activation molecule family member 1 ( MGI:1351314)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014010. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014010_01	euxassay_014010_02	euxassay_014010_03	euxassay_014010_04

euxassay_014010_05	euxassay_014010_06	euxassay_014010_07	euxassay_014010_08	euxassay_014010_09

euxassay_014010_10	euxassay_014010_11	euxassay_014010_12	euxassay_014010_13	euxassay_014010_14

euxassay_014010_15	euxassay_014010_16	euxassay_014010_17	euxassay_014010_18	euxassay_014010_19

euxassay_014010_20	euxassay_014010_21	euxassay_014010_22	euxassay_014010_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18721_wholemount_strong.wlz

18721_wholemount_moderate.wlz

18721_wholemount_weak.wlz

18721_wholemount_possible.wlz

18721_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18721_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

spotted

strong expression: see section 01 02 03 04 05 06 22 23 moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39636
Entity Detected:	Slamf1, signaling lymphocytic activation molecule family member 1 ( MGI:1351314)
Sequence:	sense strand is shown >T39636 CTAAACAAAACCCAGGAGAACGAGAATGGGACCTGCAGCTTGCTGTTGGCCTGCACAGTGAAGAAAGGGG ACCATGTGACTTACAGCTGGAGTGATGAGGCAGGCACCCACCTGCTGAGCCGAGCCAACCGCTCCCACCT CCTGCACATCACTCTTAGCAACCAGCATCAAGACAGCATCTACAACTGCACCGCAAGCAACCCTGTCAGC AGTATCTCTAGGACCTTCAACCTATCATCGCAAGCATGCAAGCAGGAATCCTCCTCAGAATCGAGTCCAT GGATGCAATATACTCTTGTACCACTGGGGGTCGTTATAATCTTCATCCTGGTTTTCACGGCAATAATAAT GATGAAAAGACAAGGTAAATCAAATCACTGCCAGCCACCAGTGGAAGAAAAAAGCCTTACTATTTATGCC CAAGTACAGAAATCAGGGCCTCAAGAGAAGAAACTTCATGATGCCCTAACAGATCAGGACCCCTGCACAA CCATTTATGTGGCTGCCACAGAGCCTGCCCCAGAGTCTGTCCAGGAACCAAACCCCACCACAGTTTATGC CAGTGTGACACTGCCAGAGAGCTGACCCATATACCCAGTGAAAGGACTTTTTGAAGGAGGATAGAAGAAC CAAAATCCACACTGAACTGGACCCCGGGTCCCAAGTTCTCTGTGACAGAAACTGCACATCTGTAACCTTC TCCAATCAGTTCCCTGGTGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93524. Forward Primer - name:093524_F_cDNA_Slamf1, sequence:CTAAACAAAACCCAGGAGAACG; Reverse Primer - name:093524_N_SP6_cDNA_Slamf1, sequence:GTCACCAGGGAACTGATTGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18720 same embryo
	EMAGE:18722 same embryo
	EMAGE:18719 same embryo
	EMAGE:18723 same embryo
	EMAGE:18718 same embryo
	EurExpress:euxassay_014010 same experiment
	MGI:4828087 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source