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EMAGE:20071

Shroom3 shroom family member 3 ( MGI:1351655)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071
"Pseudo-wholemount" of euxassay_012216. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012216_01 euxassay_012216_02 euxassay_012216_03 euxassay_012216_04
EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071
euxassay_012216_05 euxassay_012216_06 euxassay_012216_07 euxassay_012216_08 euxassay_012216_09
EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071
euxassay_012216_10 euxassay_012216_11 euxassay_012216_12 euxassay_012216_13 euxassay_012216_14
EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071 EMAGE:20071
euxassay_012216_15 euxassay_012216_16 euxassay_012216_17 euxassay_012216_18 euxassay_012216_19
EMAGE:20071 EMAGE:20071 EMAGE:20071
euxassay_012216_20 euxassay_012216_21 euxassay_012216_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20071Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20071_wholemount_strong.wlz
20071_wholemount_moderate.wlz
20071_wholemount_weak.wlz
20071_wholemount_possible.wlz
20071_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20071_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 13 14 15 16 17 19 20 21 22
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 10 11 12
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11 12
telencephalon ventricular layer
weak weak
regionalweak expression: see section 05 06 09 10 15 16 17
midbrain ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 14 15 16
spinal cord ventricular layer
weak weak
regionalweak expression: see section 11 12 13 14
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 06 07 08 09 10 14 15 16
midgut
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16
renal cortex
weak weak
regionalweak expression: see section 07 08 09 10 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36851
Entity Detected:Shroom3, shroom family member 3 ( MGI:1351655)
Sequence:sense strand is shown

>T36851
AGCACTATAGAAACCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTGACCCAGACACTCCAGGAAGGAT
CTCGCTCCGAATATCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCGGGGGGACTATGATGACGAAGTG
TTCATGAAGGACTTGCACCCCAAGGTTACTTCAAGCCCCACATTTGAAGCTCTTCCCCCACCCCCGCCAC
CTTCGCCACCGAGCGAGGAACCTCTAGTGAATGGCACAGATGACTTTCCTCCGCCTCCTCCTCCCCAAGC
CCTGTGTGAGGTACTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAGCGGCCCCTGCAGAATTCCCAGG
GTGATGGTCACAAGGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAAGGTAGTCAGATAATGACTGCCA
CACCACCCCAAACCTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGCCTTCCAGCGCGAGCGCCCAGCC
TCAGCTCAACGGTTCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCGAAACCTCACTTATGAACCTGTC
GAGAGAACTCAGGATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAGACCTCAGAAGATATCCGGACAG
AGGCTCTAGCCAAGGAAATTGTCCACCAAGACAAGTCTCTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74787. Forward Primer - name:074787_F_cDNA_Shrm, sequence:AGCACTATAGAAACCAGCCGAG; Reverse Primer - name:074787_N_SP6_cDNA_Shrm, sequence:CCAGAGACTTGTCTTGGTGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20068 same embryo
 EMAGE:20067 same embryo
 EMAGE:20066 same embryo
 EMAGE:20070 same embryo
 EMAGE:20069 same embryo
 EurExpress:euxassay_012216 same experiment
 MGI:4828066 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS