EMAGE mouse gene expression spatial database

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EMAGE:20172

Ermp1 endoplasmic reticulum metallopeptidase 1 ( MGI:106250)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012449. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012449_01	euxassay_012449_02	euxassay_012449_03	euxassay_012449_04

euxassay_012449_05	euxassay_012449_06	euxassay_012449_07	euxassay_012449_08	euxassay_012449_09

euxassay_012449_10	euxassay_012449_11	euxassay_012449_12	euxassay_012449_13	euxassay_012449_14

euxassay_012449_15	euxassay_012449_16	euxassay_012449_17	euxassay_012449_18	euxassay_012449_19

euxassay_012449_20	euxassay_012449_21	euxassay_012449_22	euxassay_012449_23	euxassay_012449_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20172_wholemount_strong.wlz

20172_wholemount_moderate.wlz

20172_wholemount_weak.wlz

20172_wholemount_possible.wlz

20172_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20172_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 05 06 07 08 16 17 18

naris

weak

regional

weak expression: see section 07 08 10 11

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 06 07 08 09 10 14 15 16 17

lower jaw incisor

weak

regional

weak expression: see section 06 07 08 11 12

lower jaw molar

weak

regional

weak expression: see section 06 17

upper jaw incisor

weak

regional

weak expression: see section 07 12

upper jaw molar

weak

regional

weak expression: see section 05 06 17

bladder

moderate

regional

Expression in the fundus region.

urethra of male

weak

regional

weak expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31341
Entity Detected:	Ermp1, endoplasmic reticulum metallopeptidase 1 ( MGI:106250)
Sequence:	sense strand is shown >T31341 ACTGGTCCTTTCCCTCGGCGTGGGTTTCCACCTACAGTCTCTTTGTGTTTTAATCTCGCGGATGGACTTT GAGTACATGTCCCCTGAACATTGCATGGGATAGTTTCTCTCCCATGATACAAGGATGTTTGTAATACAAG TCAATGAACTCTACTGAGCATATGTTCAAAGAGCTTTTGGGATAACACTCTTCAGGGCTAAGTACTGTAT GGGTTAAACTTGGGGACAGTGATACCTGGCCATGCCGGTTGAAACTTCCTGTTTTCTGACAACTAAGCAC ATGTGGATATTGCCAATCATGTCACTCATGACTAAGGACTCAGCAGCATGCCACTTGAGCAGCTTTCCTG TGAGGATCAAGAAAGATGTAGGCTCTTAGACCACTGCTAATGAAATAGGCAAGTCCACTTGCCAGTGGAG GTGGGTAAGATGCCTCTCTGTGCGCTACAACTGTAGTACTGGGAAGTAAATGCCACAAAGGTGGAAGCCG TTGCAGCACCTTAGATGCCCGAGCGCTGTTTCTTCAGGCAGCCATTCAGGTGTTTTTGTCTGTTTGGAAG CCTAAGCCCTGATGCAGAGGCCTGACTCCTGAGTAGCATTCAGTGTCTGCTTTTCCCTCAAAAACATTCC TGTCATTTACCTGGGGAAGAGAACATTTTTTTCAGCTACATTTTCTTCTTACATTTCATGGGTTGCTTTA TTTGTGCAAGCACAGAACTTAAAACTTCTTCCCCAGAGCACTGAATTGCCAGCATAGCTGCTGAGGTGGA AGAGGAGGAGGGCTGGGAGGACTGGGGATTTTATAATATTTATTCTGTTATATATAAGATTAATCCTTTA GCACAAGTTCTTTATTGCTGCATTGGGTTTTCTGTTTTCTAGCAGGTGACTAAGTTGTCGTAACTTGCCG TCAGTGAGAACCGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3987774), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21981. Forward Primer - name:021981_F_IRAV51-54_L08_D19Wsu12e, sequence:ACTGGTCCTTTCCCTCGG; Reverse Primer - name:021981_R_SP6_IRAV51-54_L08_D19Wsu12e, sequence:AGCCGGTTCTCACTGACG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20169 same embryo
	EMAGE:20171 same embryo
	EMAGE:20168 same embryo
	EMAGE:20170 same embryo
	EMAGE:20173 same embryo
	EurExpress:euxassay_012449 same experiment
	MGI:4824615 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source